GOO Events/Flower Talk Lesson 2

Lesson 2 Log

This was the log for Lesson 2 Sea Lily Genome Theory

GOO Guide to Genetics Lesson 2

Dreasimy: ~~~Welcome Everyone to our 2nd installment of the GOO Guide to Genetics :D Ariella has created a lesson plan and this is the 2nd installment. You can get more info about what to read and what homework to perform ahead of all of the classes here:~~~ Dreasimy: http://www.atitd.org/wiki/tale4/GOO_Events/Lesson_GOO_Guide Dreasimy: After each lesson Ill be posting a log for future reference :D Dreasimy: Welcome Ariella, I'm excited about todays class :D Ariella: Hi y'all :) Ariella: Okay, ready to think flowery Ariella: http://www.atitd.org/wiki/tale4/Flower_Genome_Theories#Sea_Lily_Genome_Theory Orrin: Miss! My otter ate my flowers! Orrin: I made them, honest I did! Ariella: In case y'all don't have the page on paper AliceBlue: hi :-) Ariella: yeah right ;) In the corner of the cliff Orrin Orrin: Awwww! Miss! Ariella: We'll do a little reveiw just to make sure you remember a few things. Ariella: What are the primary colors? cyan, magenta, yellow Ariella: and the answer was not supposed to be there lol but...so those are the primary colors Ariella: What are the secondary colors? Orrin: No black? Or is that formed by a combination? Ariella: that is formed by a combination which we'll get into that later Orrin: OK ty :) Ariella: Can anyone tell us what the secondary colors are? there are 3 Dreasimy: green blue indigo and violet? (cheats by looking at Genetics for dummies) Ariella: lol okay, close. you got two so you get a B Ariella: They are green blue and red Ariella: What makes each of those colors? What combinations of primary colors make each of those 3 secondary colors? Dreasimy: well yellow and cyane make green but Im confuzzled on the other 2 Ariella: Those do make cyan. Anyone know the combos for the other two secondary colors? Try and guess intuitively too Rania: i thought cyan was blue so really confussed Ariella: Those do make green, sorry Ariella: It is confusing because you're used to kindergarten and learning the primary colors. But computers are not the same. Ariella: Cyan looks blue yes. Ariella: Magenta and cyan makes blue Ariella: Magenta and yellow makes red. Rania: magenta looks red right ? Ariella: And cyan and yellow makes green Ariella: Remember ther RGB screens? red, green, blue Rania: maybe i need computor 101 before this Ariella: These primary and secondary colors are used in the flowers and you need to remember those. hitoriki: easyest way to remember for me is thinking about how a printer works and gets his colours Ariella: How many shades of a single color can there be to make the deepest shade? Ariella: yes, excellent hitoriki :) hitoriki: i think it was 4 not sure tho AliceBlue: cayan = aqua and magenta = fuschia Ariella: For egypt color rendering on flowers cyan is a primary and not made up of other colors Ariella: technicaly that is correct alice Ariella: Now let's go to lily genomes themselves Ariella: http://www.atitd.org/wiki/tale4/Genomes AliceBlue: have you ever made an aqua (cyan) flower Ariella? AliceBlue: and when you say deepest shade do you mean out to the primary? Orrin: Technically, CMYK is another way of looking at colour instead of RGB. Rania: so Bluch is magenta Tunnen: It's additive vs subtractive colour.... link to follow with picture, for those that care Tunnen: http://ian-albert.com/graphics/addsubcolor.php Ariella: How many genes long is the Clarity genome? Ariella: I have made a cyan flower yes. But if you look at the lily genomes there are less gene sets in lilies for cyan so harder to get the correct genes assembled. GiausBaltar: I count 12... Rania: clarity is 36 Ariella: Deepest shade as in...if you build a purely magenta shade 1 flower then a shade 2 and so on. Ariella: yes, additive vs subtractive, but that is technical. For what you need to do advanced cross breeding you can get along fine with knowing the 3 primary and secondary for now. But it is interesting reading. Ariella: yes Clarity is 36 genes Ariella: Which sea lily has the shortest genome and how many genes is that genome? Maybe someone who hasn't answered yet? Ariella: http://www.atitd.org/wiki/tale4/Flower_Genome_Theories#Sea_Lily_Genome_Theory Ariella: It's okay to cheat and look ;) Ariella: oops, sorry wrong page Ariella: http://www.atitd.org/wiki/tale4/Genomes Crestalina: vampire = 12 Ariella: yes :) Ariella: Which sea lily has the longest genome and how many genes is that genome? Rania: that we have? Ariella: Yes, of the original bulbs from UWorship Rania: dusk has longest but we dont have that lily Ariella: this tale Rania: then morning at 44 Ariella: Dusk was the longest last tale yes. But which from this tale? Ariella: right :) Ariella: Which lilies have one or more 'V' genes in them? Rania: guessing fracture Ariella: Don't be shy y'all those haven't answered. Throw out some answers Ariella: Not Fracture, no. Crestalina: bluss, crown energy ;-) Ariella: Take a look at the Genomes page again. AliceBlue: why are they grouped ion different amounts? Ariella: Right (and it's Blush) Crestalina: lol Ariella: What does the 'V' gene control in a sea lily? Rania: amount of ferts? Ariella: Grouped in different amounts where? AliceBlue: like energy has 5 lots of V each end and eight groups of four in between Ariella: Yes, V is a gene set by itself. One V gene lowers the flower fertilization rate by 2 normal flower fertilizations or 1 sticky sun fertilization. AliceBlue: gene sets have different amounts of letters in them, why is that? Pascalito: The groups of V's are irrelevant Ariella: Because that's how the dev decided to group them in Energy. Vs are single gene sets by themselves so it has no relevance whether they are scattered in a genome or packed together. Rania: lost again Pascalito: Alice, again a decision made by the devs Ariella: Alice have you programmed? AliceBlue: no programming no Rania: if V makes less ferts and blush has most V then why does energy take less fert AliceBlue: ok dev thing Pascalito: Energy has most Vs Ariella: okay, probably you know what a command is in programming? <<<oops missed some of this chat in my log, sorry>>> AliceBlue: does Silken have only one gene set? Ariella: So we saw that the max for Vs to lessen fertilization is 12. Crestalina: after 12 it dosent matter anymore Pascalito: Silken has no gene sets at all, poor thing, does not speak our language i guess ;-) Ariella: Silken has no gene sets. It's like a pot of alphabet soup that makes it nice for mutagen building later. Pascalito: tht is why Silken is white and regular size and 48 ferts AliceBlue: the wiki is a little confusing because it talks about the genomes separating into gene sets Ariella: What is the default number of regular fertilizations a sea lily has if it has no 'V' genes? Crestalina: 48 AliceBlue: so how are sets defined, if not by the grouping of the letters? Rania: and what are the different sets? Ariella: Alicewehadtofigureoutwhatthegenesetswereineachgenome. AliceBlue: heheheheh Ariella: That make it easier? heehee Ariella: We'll be getting into the different sea lily gene sets in a bit. Ariella: How many fertilizations of sticky sun would equal that 48 of regular fertilizer? Rania: 24 Ariella: good :) Ariella: So it cuts the split time in half. Rania: you would think lol Ariella: How many regular fertilizations does Blush have? Ariella: and why? Rania: still takes FOREVER Ariella: Sticky sun fert cuts ferting in half. Ariella: lol yes it does ;) Rania: 26 and because of the V genes? Ariella: That is the length of the genome. GiausBaltar: 38... 48 ferts - 10 (from 5 V genes) = 36 Rania: ooops 38 and still the V genes Ariella: Another try? Ariella: yes giaus :) Crestalina: 38 because of 5 V ( 1V is minus 2 Fert, so 5X 2 = 10 less) Ariella: What gene do you see at the beginning and end of each genome? GiausBaltar: err... = 38 at the end, not 36 Rania: K = Black Ariella: good :) Rania, you kept me from asking the whole name of that gene ;) Ariella: Since there are two gene names that start with the same letter it was decided to use K for Black and U for Blue. Ariella: The K (Black) gene always starts and ends a genome. Ariella: When counting the number of genes we never count the K gene. Think of it like parenthesis around the genome (IOIOIYIOIYIY) Vampire sea lily. Ariella: Now for a second of review. Ariella: http://www.atitd.org/wiki/tale4/Genetics_for_Dummies Ariella: What gene name does V stand for? GiausBaltar: Can the K gene still be mutated into the middle of a genome? Rania: violet ? Ariella: No the K gene cannot be moved anywhere else in a genome. Unless there's a bug ;) Ariella: Yes, violet :) Ariella: Gene names happen to be the names of colors. DO NOT get those color gene names mixed up with the colors that show on a flower. Gene sets (groups of genes) control size, color, number of flax, etc on a plant. Pascalito: Look at the K as just an indicator of the ends, not as a physically existing gene Rania: but violet is not a color just the name of gene right Ariella: Reveiw over...time to get a little more advanced. <<<Meh sorry did it again, this is scrolling faster than I thought>>> Ariella: The K (Black) gene always starts and ends a genome. Ariella: When counting the number of genes we never count the K gene. Think of it like parenthesis around the genome (IOIOIYIOIYIY) Vampire sea lily. Ariella: Now for a second of review. Ariella: http://www.atitd.org/wiki/tale4/Genetics_for_Dummies Ariella: What gene name does V stand for? GiausBaltar: Can the K gene still be mutated into the middle of a genome? Rania: violet ? Ariella: No the K gene cannot be moved anywhere else in a genome. Unless there's a bug ;) Ariella: Yes, violet :) Ariella: Gene names happen to be the names of colors. DO NOT get those color gene names mixed up with the colors that show on a flower. Gene sets (groups of genes) control size, color, number of flax, etc on a plant. Pascalito: Look at the K as just an indicator of the ends, not as a physically existing gene Rania: but violet is not a color just the name of gene right Ariella: Reveiw over...time to get a little more advanced. Ariella: http://www.atitd.org/wiki/tale4/Advanced_Crossbreeding Ariella: Yes, violet is a name of a gene with no meaning of color in this application. Ariella: First, did anyone have trouble naming your baby flower at a UWorship? Dreasimy: ya I forgot to get seed registry! :D thought I had it already, then I had to come up with a nifty name, I picked Pointedly Purple and Frangrant Hot Tub ;D Ariella: Those of you being very quiet feel free to throw in questions :) Rania: i had trouble too ........ getting there Ariella: That is cool Dreasimy! lol I love naming. My sweet hubby let's me do the renaming. Numbers don't cut it ;) Ariella: Let's start with the first homework cross...Clarity/Vampire. Notice the syntax I use...Clarity is Left Splint and Vampire is Right Splint plant. It is essential you record your crosses. AliceBlue: this one is so cool I got AliceBluepetals Ariella: How many genes are in the Clarity genome? Dreasimy: 36? Ariella: yep Ariella: How many genes are in the Vampire genome? Rania: 12 Ariella: To know how long a child flower or plant will be you add those two numbers. Then divide by 2 to get the average. Lastly, if the resulting answer is not an integer (a whole number like 3) but it is a fraction like 23.5 then round up to the next whole number or for this example 24. You now know the genome length of your child flower. Ariella: So for the first cross the child flower length is...? Dreasimy: 24 Ariella: how did you get that? Dreasimy: erm by 36 (clarity) plus 12 (vampire) = 48 divide by 2 = 24 (I think....) Ariella: lol yes :) perfect Rania: what i got too Dreasimy: phew Ariella: Someone tell me what the second homework cross child flower genome length would be and how you got it? Rania: 27 ? Dreasimy: Rania didn't show her work! <whines> Ariella: yes and how did you get it? Ariella: Dreasimy, please raise your hand first if you are going to tattle. Rania: 36 + 18= 54 divided by 2 is 27 Ariella: Or you might have to sit with Orrin. Dreasimy: <gulp> Ariella: Perfect :) Rania: i want to sit with Orrin Ariella: There are two exceptions to this. Orrin: Hello! :D Ariella: You'll learn in a little while that there is a splice point where the two plant genomes from the Left and Right Splint plants are truncated and put together or spliced. At that point a gene can be deleted or it can be duplicated and added in, thus either shortening or lengthening a genome by 1. Now take a look at your first cross, the Clarity/Vampire cross and go to the flower theory page. Ariella: wonders why but is NOT going to ask ;) Ariella: So we are all good with understanding how long the child flower genome is? Ariella: Then let's go back to the flower theory page. Ariella: http://www.atitd.org/wiki/tale4/Flower_Genome_Theories#Sea_Lily_Genome_Theory Ariella: Keep in mind the primary and secondary colors on flowers. Ariella: At the first portion of the Sea Lily Genome Theory you'll see that the genomes of each sea lily have been broken into gene sets. Ariella: I think Alice was asking why we did this. I only put it on the theory page where it explains exactly what gene sets control. Ariella: What properties can gene sets control in plants, not just flowers? One person at a time please say one property and different people speak up. Dreasimy: Inner west petal set? Ariella: Tunnen you've been working with wheat. What is something a gene set controls in wheat? Crestalina: how many flax you get Ariella: yes dreasimy :) Ariella: color in that petal set. Tunnen: EEp, pop quiz? I wasn't ready for this =P Ariella: yes crestalina :) Ariella: lol too bad, answer up or sit with Orrin. hitoriki: size of the flower tiny or huge :-) Tunnen: GR seems to increase wheat yeild output.. Ariella: size in flowers is one thing Ariella: fertiliztion rate as you've found out. Orrin: Hey now! that's not fair! Ariella: number of grapes in vines Ariella: orrin, if you can think of a control you get out of the corner ;) Tunnen: ORRGY or ORRGYU seems to be required to grow on sand.... but there are some further tests I still need to do Ariella: type of soil wheat is grown in Orrin: haha... ORGY... Hahaha Ariella: amount of sugar each tend gives Tunnen: To grow on dirt, it looks like you need at least 1 GR and a RY, again... more testing needed. Ariella: haha AliceBlue: seeds from flax Ariella: places dunce cap on Orrin Ariella: yes alice and Tunnen Rania: Orrin give me back the dunce hat!! Ariella: number of waterings in flax Tunnen: and Grass seems to have something to do with the are around the OYOY set... I was about to work on that next, before taking a wheat break to work on my Funeray Temple Orrin: yay! It has pretty flowers on it :D Orrin: I grew 10k wheat, I shant touch it again this tale. Ariella: Someone name a gene set...even if you don't know what it does yet. Ariella: Excellent Tunnen! Thanks. That is how we have to start thinking when we first see how to even progress on a new genome type. Tunnen: isn't ROYG one... Rania: gror? AliceBlue: my hubby said gror ought to be grow :-) Ariella: sure is :) Tunnen ROYG makes a lily a giant Ariella: yes rania :) Tunnen: I'm also betting clay has something to do with the KKK area.... but we'll know in a few days... I should hopefully get back into some last min wheat testing this season, and a bunch at the start of the next season. Ariella: The next area of the Sea Lily Genome Theory is getting you ready to know how gene sets are arranged. There are three components in sea lilies that gene sets control...color, size, fertilization rate. Ariella: Gene sets controlling color in sea lily petals are always 4 genes long. The first gene in the set of 4 codes whether the outer or inner petals will be colored. The following three genes control what color will be used on that petal set. Ariella: There are four different petal sets. Two outer and two inner. Look at Crown when you have time. You'll see that three alternating petals are colored the same. Ariella: The two outer petal sets begin their gene set with G. On the Flower Gene Sets page you'll see that we notate it as Gxxx. The gene set that colors petals in sea lilies is always 4 genes long, if you remember. The two inner petal sets begin their gene set with U and the entire gene set is notated as Uxxx. Ariella: The two outer petal gene sets are coded as Gxxx and Gxyz where the xxx are the same color gene (like GOOO or GYYY or GRRR) and xyz (like GROR or GORR or GRRO). The two inner petal sets are similar. Either Uxxx or Uxyz. Ariella: Is your mind boggled yet? Any questions? Dreasimy: When 2 peple do the exact same cross as we all who did our homework allegedly did, should they all be the same or does something random happen too, i.e. are there lots of my Pointedly Purple out there now named other things? :D Dreasimy: my flower is all white I think except the inner circle is purple, darker purple than blush's purple Dreasimy: and it's normal sized Ariella: Excellent question. AliceBlue: less boggled now than before the class, but still a bit boggled Ariella: This is what advanced cross breeding is to find out. The simple answer is that something random happens. Ariella: lol Alice Ariella: Yes you can get the exact same child genome as others but there are quite some other possible genomes. Ariella: We'll get to that in a minute. Dreasimy: cool Rania: is there a page written that has the different gene sets for us to look at in future? Ariella: Stamens. Ariella: This seems to be the most confusing concept. Gene sets for this are Ix, Ixx and Ixxx possibilities. 'x' only represents the 3 possible primary color genes. AND REMEMBER THAT A GENE NAME DOES NOT MEAN THAT COLOR IS WHAT IS ON THE LILY. It's only a gene name. Unfortunate choice. The lily stamen has 3 parts to it (it sort of looks like 4 but it isn't), the outer (top portion), middle and inner (the very bottom portion). Ix colors the outer stamen. Ixx colors the outer and middle stamen. Ixxx colors the outer, middle and inner stamen. Ariella: Run to a flower Ariella: That itty bitty tip on top is part of the outer stamen. Had that question a million times so I'll answer it now. Ariella: Example: Ix and Ixxx in one genome would color the outer stamen shade two and the middle and inner stamen shade 1. Everyone get that? Dreasimy: sorry I dont :D trying tho :D Ariella: Or let's put in a gene from the Color Key, say O gene controls magenta. AliceBlue: the little x are a bit confusing Dreasimy: if only my Ix has color (my outer stamen) then that means only my Ix has color and my Ix and Ixx have none cuz they wer white? Ariella: If a genome had both IO and IOOO then IO controls the outer stamen (the topmost region) Pascalito: cause the "word"or gene set IO is in there twice it is shade 2 Ariella: You're familiar with algebra? Ix for stamens and Gxxx for outer petals and Uxxx for inner petals. Ariella: I'm calling your attention to the first letter of the gene set. Rania: x means unknown ? Pascalito: in a way, it can have different values Ariella: If there was only a G or only a U or only an I it would not be a controlling code or gene set. Rania: and ppls want to know if this is chat is being logged Ariella: x means that any of the genes in the color code can be inserted. Dreasimy: yes it's being logged (tho I have missed a few parts, it scrolled a little faster than I expected at times) but yes :D Ariella: I should be able to fill in missing parts. Ariella: So FIRST notice on flower gene sets the first gene. Ariella: So you know what part of the flower that gene set controls. Ariella: Does that help? Tulani: So is this right: you can only colour the inner stamen the same colour as the outer stamen, and you cannot colour the inner without also adding a shade to the outer? Pascalito: you could make a complete list of all existing gene sets (words, strings that mean something), but using the xxx or xyz notation makes it more compact Ariella: yes tulani :) Ariella: yes on gene set that is true. Ariella: Think of gene sets as magic words that make things happen. Pascalito: inner and outer can be different, but inner is always a subset of the outer stamen colors AliceBlue: I have a colour on the outer and middle but not the inner Pascalito: in other words, outer stamen can have only more colors on it, not less than inner stamen Rania: same Ariella: the only genes that come after the I or G or U that makes things happen are O for magenta, Y for Yellow (only coincidence the gene name and color matches in lilies), and R for cyan. Dreasimy: I think I just have it on the outer, which is the tippy top AND the part below that right?> Pascalito: yes Dreasimy :) Ariella: Now visually you will be able to see a different color on outer and middle and inner stamen. Ariella: Now visually you will be able to see a different color on outer and middle and inner stamen. Ariella: In this first cross I think only with an added (duplicated) gene at the splice point will it be possible to see a color on the middle stamen. But it's good you're thinking this way. Ariella: Two different issues are working here. I was explaining single gene sets and what they do. Ariella: IO. I for the stamen. Ix. x=O in this example. IO only one O so only the outer stamen is colored. and the O is magic for coloring a lily magenta. AliceBlue: So Dreasimy and I have an I XX? AliceBlue: where x is a something Ariella: IOO. Ixx in this example x = O. I for stamen control. OO for coloring both outer and middle stamen magenta. Pascalito: if you are sure the middle stamen is colored, yes AliceBlue: right Dreasimy: only my outer one is so Im IO? Pascalito: Dreasimy said only outer stamen has color, Rania said middle i think AliceBlue: an IOO and if all parts of the stamen have colours an IOOO set Ariella: IOOO. Ixxx in this example x still equals O. I for stamen control. OOO for coloring all the stamen, outer, middle, and inner magenta. Rania: i have outer colored and middle colored AliceBlue: tip and middle with colour, iinner no colour Ariella: What color is the middle stamen? Ariella: I think it can only be yellow for that first cross. AliceBlue: gray (lol) Pascalito: ok, which is possible indeed for this cross, though rather exceptional :) Rania: mine is purple so would be O Ariella: hmm, that would be impossible. Ariella: so maybe you are seeing only the outer stamen Pascalito: middle stamen cannot be gray on this cross, you probably still see the outer stamen there Ariella: Now let's get into the other factor about stamens. Pascalito: remember, outer stamen is 2 upper parts together Dreasimy: The stamen sounds like a 4 tiered cake, bottom tier is inner, middle tier is middle and the top 2 teirs (which include just a pointy thing sticking up) is outer Rania: let me doulbe check wiki on stamen AliceBlue: oops confused about inner and outer Ariella: You'll see on both Clarity and Vampire that we have stamen gene sets with three different colors. Sorry, Vampire has two colors included on their stamen gene sets. AliceBlue: aw so I only have an IO gene set Ariella: exactly, Dreasimy :) Pascalito: no Alice, you sure can have more than just IO Ariella: Layering. Rania: ok i was wrong too Rania: only color on outer Ariella: This can happen in petals, leaves, whatever part of the flower can be colored. Ariella: IO would color outer stamen magenta. IY would color outer stamen yellow. Put that together and you get a secondary color...red. For Vampire :) Ariella: and you will notice that Vampire has 3 gene sets of IO and IY making the outer stamen shade 3 red or Red3 as we abbreviate it. Rania: ok question Dreasimy: what color is blush's outer stamen naturally? cuz mine is darker than that and Im confused wht to call it :D Ariella: Black on the screen is usually seen as grey until it hits shade 4. Rania: mine has just the outer stamen colored so i have IO xx? Pascalito: Blush outer is Mag3 AliceBlue: the cross has exactly the same as clarity, so I have the set IRIR IO IO IY IY? Ariella: Rania, you might have more than one IO Pascalito: yes Alice, very possible ! AliceBlue: ty <<<Missed out some content here, sorry>>> AliceBlue: if it were lighter gray would it be IR IO IY? Ariella: It has black (gray) petals and outer stamen. AliceBlue: yes :-) Ariella: Does that make sense now? Pascalito: indeed AliceBlue Pascalito: and if you ever wanna see a true black (shade 4), visit us in HG :) AliceBlue: sure will yes! Ariella: Everyone catching on? Dreasimy: so if blush is mag3 I think mine is mag4 since mine is a darker purpley Dreasimy: and the middle one has no color to further affect my outer? <hopes she is catching on> Ariella: not necessarily Dreasimy. You might have a cyan IR in there. Pascalito: could very well be Dreasimy Dreasimy: okie Ariella: Last thing on gene sets. Rania: mine is a "dirty purple" Ariella: Size. ROYG in the genome makes a lily a giant. GYOR makes it a dwarf. If you have two ROYGs and one GYOR in a lily they cancel each other out and you get a normal size lily. Which lily does that? Ariella: yes, dirty is black underneath :) Rania: fracture? Ariella: yes :) Pascalito: yes Rania, probably due to grey base in it Ariella: Back to the advanced cross breeding Ariella: http://www.atitd.org/wiki/tale4/Advanced_Crossbreeding Ariella: YOu guys need a potty break or anything? Ariella: I guess I'll let Orrin out of the corner. He's behaved very well. Dreasimy: The mod could use a very brief break :D like 3 minutes Pascalito: We're getting scared about the bill for extra broadcasting time... Ariella: Okay, 5 min break....if you're late break you get demerits. Dreasimy: hehe Dreasimy: Mod is back, fyi :D Rania: me too Rania: watch the teacher be late lol Orrin: *has been trying to make and drink beer in the corner* Pascalito: the assistant is back Ariella: oh oops, lol I'm back Rania: no apple for teacher she is late Ariella: everyone back? I know this is a long session. But it will be easier on the other flowers. Ariella: awww, I love apples :( Ariella: The first cross you made was Clarity/Vampire...Clarity Left Splint and Vampire Right Splint. You should have recorded that and made very sure that you put them in the correct splints. Ariella: Someone put the Clarity genome here...without the beginning and ending Ks. Dreasimy: I feel 96.7 percent sure I did that ;) Dreasimy: IRIR IOIO IYIY GORR GROR GRRO GORR GROR GRRO Ariella: Someone put the Vampire genome here. Dreasimy: IY IY IO IY IO IO Dreasimy: (I tried to wait for someone else, they are being shy!) Ariella: Try and use Notepad or if using a wiki page indent one space. Indenting one space on the wiki will give a non true type font so that each alphanumeric character is spaced the same and the genomes will line up under each other. Or you can just use a piece of paper but line up the letters. Orrin: This is one occasion where I would recommend the use of a spreadsheet for non-numerical work Ariella: this is the very cool part of understanding cross breeding. The letters of the genes need to line up under each other. So just grab a paper and pencil. Ariella: So put the Left Splint genome on one line (Clarity) and the Left Splint genome under the that. Ariella: Next...center the shortest genome above or below the other genome. In this case it's Vampire on the Right Splint so it is under the Clarity genome. This is because it averages the length. Centering both represents that mathematically. Ariella: You center because it averages the length. Rania: you mean right slpint vampire under? Ariella: I'll let you write that out and I'll explain one more thing about centering. Pascalito: so you'll have 12 spaces before and after Vampire genome Ariella: Clarity genome is on the first line (or the imagined first line if you have a blank paper. Then put the Vampire genome, centered under it. Ariella: Make sure each gene is lined up under the other and that there are an equal number of blank spots on each side of the Vampire genome compared to the Clarity genome above. Ariella: It is very important to ALWAYS put the Left Splint genome on the first line or on top. Ariella: and the Right Splint genome under it. But you center the shortest one. Ariella: If the child flower length had been a fraction and you rounded up then you would shift the shortest genome by 1 towards the Splint side it was in. If the shortest genome was on the Left Splint you shift it 1 to the Left over the Right Splint genome. If the shortest genome was on the Right Splint then you shift it to the Right under the Left Splint genome. Ariella: Has everyone done that? Because I'm going to ask a question about it. Dreasimy: I dood it Ariella: lol Everyone dood it? Ariella: Easy example. AliceBlue: done that yes Ariella: IOI Left Splint (pretend) O Right Splint Ariella: You'd put O under the IOI Ariella: Now notice something. Start counting from the left side of the longest plant genome and then jump to the other genome when you get to it and keep counting til the end of the shortest genome. Ariella: How many do you count? Dreasimy: 36 AliceBlue: 24 Pascalito: right :) Dreasimy: oh oops ;D Ariella: Alice gets a prize Rania: lost Pascalito: the 24 is right Pascalito: 24 looks familiar ? Ariella: IRIRIOIOIYIY from Clarity then you are over Vampire genome. Dreasimy: oh oh it was the child size Pascalito: yes Dreasimy Dreasimy: which was clarity (36) plus vampiry (12) divided by 2 Ariella: so then count these on Vampire with the above....IYIYIOIYIOIO Ariella: You should notice that is the same number as the child flower genome length we figured out earlier for this cross Ariella: Lastly, you can now see all the possible genomes of child flowers coming out of the cross. You just don't realize it yet ;) Ariella: Just take your pencil or | bar key and draw diagonal lines through both genomes, starting with a line IN FRONT OF the first gene letter of the shortest genome and after each gene letter to the END of your parent genomes (a vertical line after the last gene of the shortest genome). Draw the vertical lines through both of the two genomes. Ariella: These are all your possible splice points. Ariella: If you're confused, then jump to this link to see what I mean. Ariella: http://www.atitd.org/wiki/tale4/Advanced_Crossbreeding Ariella: Let's go back to our easy example. IOI on top (Left Splint) Ariella: O Right Splint. Ariella: The first vertical line would go right after the I of IOI on top and down in front of the O on bottom. Ariella: Another vertical line would go after the IO of IOI and down after the O on bottome. Ariella: But with this Clarity/Vampire cross how many vertical lines (splice points) do you have now? AliceBlue: 13 Rania: no idea you 100% lost me Ariella: 13 is right :) Dreasimy: darn I thought it was 4 so Im confused as well :D Ariella: Rania, did you follow about lining up the two genomes? Ariella: okay, Clarity genome again Pascalito: every gene of Vampire should have a bar before and after it Pascalito: IRIRIOIOIYIYGORRGRORGRROGORRGRORGRRO Ariella: and Vampire genome Dreasimy: ohhh Pascalito: IYIYIOIYIOIO Dreasimy: ok I have 13 now Ariella: The first gene I of Vampire should be directly under the first G you find in Clarity. Pascalito: make sure you have 12 blanks in front of Vampire after centering Ariella: then line up each letter of the Vampire genome to match up with a letter of Clarity above. Pascalito: I wish we had a blackboard... Ariella: Then draw a vertical line that goes between the last Y in Clarity genome and down in front of the I that starts the Vampire genome. Rania: so you are saying the splice lines should be at begining and end of vamp? Ariella: Then continue drawing a vertical line between each pair of genes. Ariella: yes Rania :) Pascalito: there are lines there too yes, and in between all gens of vampire too Ariella: So that you end up with 13 vertical lines Ariella: The new child flower genome will be formed from the beginning genes of the Left Splint parent genome starting on the left and going until it hits one of the splice points. You won't know right now where your child flower splice point is, you'll see in a minute. We just need those possible vertical line splice points right now as an exercise. Ariella: So for the number of vertical lines or splice points you will always have the number of genes of the shortest genome plus 1. Ariella: Does everyone see that? Dreasimy: <nods> Rania: child has the IRIRIOIOIYIY for sure? Ariella: At that splice point (whichever vertical line your child flower will be), it will finish the new child flower genome by adding on the right end genes of the Right Splint parent. We aren't talking longest or shortest now, but Left Splint genome and Right Splint genome. Pascalito: yes Rania !!! Dreasimy: ooo Ariella: So on your diagram (after you look at your child flower) you will write down the genes beginning with the left side of the Left Splint parent and then drop down a line at your splice point and continue writing the rest of the genes from the Right Splint parent to the end of that genome. Rania: and the light bulb comes on Ariella: A for instance example on the Clarity/Vampire cross: IRIRIOIOIYIYGOR | YIOIYIOIO (notice that splice point) Dreasimy: see it Ariella: Possible child flower genome: IRIRIOIOIYIYGORYIOIYIOIO (just rewriting without the splice point) Rania: light went out Ariella: Now the trick is where the heck is your splice point? How do you know that? Dreasimy: psychic powers? Ariella: Remember the gene sets and how they control size and color and fertilization rate? Ariella: Rania, let's put the light back on Ariella: the above example is a for instance child flower genome Rania: where did the GOR com in ? Ariella: Did you find the 13 splice points (vertical lines)? Dreasimy: she randomly picked asplice point which contained 3 of the top line letters (the first 3 on top of vampire) Rania: the first one should be at the G On clarity right? Pascalito: first line is left of first G from Clarity Rania: ok where i have it Ariella: You can see each of the possble 13 child flower genomes Pascalito: now move to 3 lines further to right Rania: but why? Rania: and how do you know to move 3 lines to the right Pascalito: the 3 is what we did for the instance Rania: because that is the first set of genes? GiausBaltar: The vertical lines you are drawing are not the splice points, they are the *possible* splice points. The splice you get could be at any spot in between Dreasimy: Rania I think theya re just picking one splice point randomly to use as an example Pascalito: well said Giaus :) Dreasimy: it could be any of the 13 Ariella: If you take the beginning genes from your Left Splint genome (or the genome on top) until you get to a splice point and then at any of those splice points go down to your Right Splint genome and continue taking the rest of those genes. Ariella: Right Giaus and Dreasimy. Ariella: Now guess why I went over lily genome theory so you would understand gene sets? Dreasimy: cuz they are the most complicated? :D Ariella: Time to go look at YOUR lily :) Ariella: ding...no Dreasimy ;) Ariella: I chose Clarity because it has the most variation in color. It's a long genome so it's a fun one with crosses because there ARE more possible different child flowers from it. Dreasimy: <pets her flower> Pascalito: for this cross, in my humble opinion, the easiest thing to look at first is petal colors (if any) Ariella: now you see your flower in a completely different light :) Dreasimy: I gots no petal colors Pascalito: ok, what would that mean Dreasimy? AliceBlue: I got blue petals one lot outer Pascalito: look at the possible splices Ariella: so what can you guess as your possible splice point Dreasimy? Dreasimy: is there a gene for 0 color I guess that's confusing me Pascalito: Alice, sure it is blue, or is it just cyan (not easy if never seen before) Ariella: Alice is it blue or cyan or do you know? Pascalito: white is default color, so if no gene sets for the petal, it stays white Ariella: Dreasimy, you said you had no petal colors AliceBlue: lightish blue like "AliceBlue" hehe AliceBlue: I got past slice point 8? Ariella: You can see that every child flower in this particular cross WILL have the grey stamen? Pascalito: might be aqua looking then, which is cyan Pascalito: yes Alice :) Rania: i am thinking no to blue as there is no U in either parent Ariella: Because the first possible splice point comes AFTER the all the stamen stuff in Clarity? Pascalito: well, make that 5 Alice, but i know what you mean Pascalito: it could be blue ! Dreasimy: Im really drawing a blank when looking at it Pascalito: blue is possible if you got GORRGROR in child Ariella: Dreasimy, do you see that the first splice point comes after IRIRIOIOIYIY? Pascalito: anyone have a grey petal ? Dreasimy: well I thihk it must go to 13 then if I had to guess, my splice is 13 cuz I have 3 os? Dreasimy: yes Ariella: Rania we'll get ot you in a second AliceBlue: I think I have GORRGROR in the child Ariella: Dreasimy, do you see that the first splice point comes after IRIRIOIOIYIY? Pascalito: anyone have a grey petal ? Dreasimy: well I thihk it must go to 13 then if I had to guess, my splice is 13 cuz I have 3 os? Dreasimy: yes Ariella: Rania we'll get ot you in a second AliceBlue: I think I have GORRGROR in the child Pascalito: that is a shade 1 blue Alice Pascalito: you can try to double check with stamen color now for your possible slices with blue petal AliceBlue: ah so less than that Ariella: So since you have no color in the petals the splice point range can be narrowed down to 4 possible splice points Dreasimy: like right now Im looking at the Lily Color Gene Sets on the Sea Lily Genome Theories page, is that what I should be looking at? Dreasimy: if so I dont see how to apply it to my possible splints :D Ariella: Dreasimy, plus either including the G or GO or GOR from Clarity Pascalito: yes, to compare strings of gene sets to colors you see Pascalito: and admitted, it is darn hard to determine the colors in the outer stamen for this cross generally, cause lots of shades Dreasimy: ok let me look again Ariella: or including nothing from Clarity but the stamen gene sets Pascalito: anyone has same grey stamen as Clarity ? Dreasimy: ok when it says outer south petal (that's not for stamens that's for petals, where do I find the thing for stamens?) :D Sorry AliceBlue: yes same gray as clarity Pascalito: first thing after Lily Color Gene Sets title Ariella: Stamen gene sets...IR IO IY first thing under Lily color gene sets Pascalito: ok ALice, that means a lot ! Dreasimy: ok so if Im IO would that put me at the 5th splice point? Pascalito: means you got no extra outer stamen gene sets from Vampire, meaning it took none or just 1 gene from Vampire AliceBlue: splice point 13? AliceBlue: nothing from the vampire? Ariella: well, remember for the child flower genome you start with the beginning genes in order from the Left Splint parent genome. Pascalito: and since you have not a grey petal set, it took 1 from Vampire, to "kill the GORR Pascalito: meaning we know Alice's genome ! ... well her flower's :) Dreasimy: ya I think my gray petals got killed off by something in vampire Ariella: Dreasimy, You will definitely have IRIRIOIOIYIY right? Dreasimy: ooo wait Dreasimy: I think I have a blueish petal Pascalito: aha, interesting ! Ariella: and what color is your stamen? Dreasimy: I thought they were all white Dreasimy: outer stamen is a purple darker than blush's rest is white Dreasimy: but I didnt notice this petal before its blueish Pascalito: the last gene from Vampire is O too, same as 24th of Clarity Ariella: okay, a very light blue? Pascalito: measn Alice had a subtracted gene Dreasimy: well it wasnt very obvious to me until I started comparing the clarity to the Pointedly Purple AliceBlue: plice point 12 or 13 and the O on clarity and O on vampire not in description Dreasimy: without another blue to compare it to Im not sure ifits light blue or not Ariella: GORR is cyan Dreasimy: I think its on one of the outer petals Pascalito: is you can hardly see, it probably is just Cyan Ariella: after the stamen gene sets in Clarity you have GORR then GROR AliceBlue: so description of child IRIRIOIOIYIYGORRGRORGRR pasc? Dreasimy: well its very in my face now but I didnt see it for a long time :D Ariella: GROR is magenta Pascalito: yes, has to be an outer petal set Ariella: so it might be cyan (which looks like light blue) or a blue (a combo of the cyan plus magenta) Pascalito: shade 1 yellow is even harder to notice Dreasimy: and why do I only see it on the top part of one petal but I think I see it on a few undersides of other outer petals? Pascalito: a lot depends on daytime light settings and your video card Ariella: Dreasimy, let's see which petal set it is. Ariella: Is the colored petal set north or south? Dreasimy: ok it *looks* like right outer? but Im not sure Dreasimy: the one petal that has blue on the top side is east Pascalito: need to look at N and S petals Pascalito: remember, F4 for an arrow pointing to N Dreasimy: ok underside of north petal is blue Dreasimy: I thought they were all white Dreasimy: outer stamen is a purple darker than blush's rest is white Dreasimy: but I didnt notice this petal before its blueish Pascalito: the last gene from Vampire is O too, same as 24th of Clarity Ariella: okay, a very light blue? Pascalito: measn Alice had a subtracted gene Dreasimy: well it wasnt very obvious to me until I started comparing the clarity to the Pointedly Purple AliceBlue: plice point 12 or 13 and the O on clarity and O on vampire not in description Dreasimy: without another blue to compare it to Im not sure ifits light blue or not Ariella: GORR is cyan Dreasimy: I think its on one of the outer petals Pascalito: is you can hardly see, it probably is just Cyan Ariella: after the stamen gene sets in Clarity you have GORR then GROR AliceBlue: so description of child IRIRIOIOIYIYGORRGRORGRR pasc? Dreasimy: well its very in my face now but I didnt see it for a long time :D Ariella: GROR is magenta Pascalito: yes, has to be an outer petal set Ariella: so it might be cyan (which looks like light blue) or a blue (a combo of the cyan plus magenta) Pascalito: shade 1 yellow is even harder to notice Dreasimy: and why do I only see it on the top part of one petal but I think I see it on a few undersides of other outer petals? Pascalito: a lot depends on daytime light settings and your video card Ariella: Dreasimy, let's see which petal set it is. Ariella: Is the colored petal set north or south? Dreasimy: ok it *looks* like right outer? but Im not sure Dreasimy: the one petal that has blue on the top side is east Pascalito: need to look at N and S petals Pascalito: remember, F4 for an arrow pointing to N Dreasimy: ok underside of north petal is blue Ariella: so that is GORR Dreasimy: and I already thought I had IO for sure from vampire because of the outer stamen being magenta-y Ariella: the question is, is it cyan or blue. From your stamen coloring it seems like it is probably just the GORR Dreasimy: so ddoes that mean its the 5th split? Dreasimy: is there any flower I might have with one of the 2 colors? Dreasimy: so I could compare? Pascalito: yes, probably 1 or 2 IO from Vampire Dreasimy: oh here's something I have called Pyramid Blue, is this yours? Dreasimy: this blue is darker than mine Ariella: purple would stick with magenta so find the splice points where you will not include an IY in your child genome Ariella: yea Pyramid Blue is mine Dreasimy: so your blue on the north outer is darker than mine is if that helps hehe Ariella: you can include Y then IOIO because Y does nothing without the I Pascalito: yes, but that is ahde 2 blue Ariella: yes that does help I think Pascalito: *shade Ariella: Anyone who wants help with interpreting their flower is VERY welcome to bring it to HG and plant it and we'll go over it :) Dreasimy: cool :D Dreasimy: maybe next homework it would be good to plant one of our bulbs for you to see before class :D Pascalito: I think it might be best to go over the individual results after the lesson in private if needed, or after you looked at the homework solution text i prepared, and will add to end of the page with this lesson Ariella: Dreasimy, so you think you see the possible splice point range? Dreasimy: no Im still confused but let's move on :D Ariella: after the GORR in Clarity, but after the I in the last IY in Vampire Pascalito: it is sometimes hard, especially if nothing to compare with Ariella: Remember I said that an added (duplicated) or subtracted gene could happen at the splice point. This can possibly transform a gene set into an entirely new gene set that the parent flowers did not carry. Also, the splice point can form a new gene set that the parent flowers did not have before that triggers a different color or size that the parents did not have. Dreasimy: ohhh okie :D neat Pascalito: i have a giant6 with a cyan petal in HG Ariella: Something to point out on lilies while you eyeball your child flower... AliceBlue: Great idea Dreasimy. plant one at the classroom Ariella: Color gene sets on lilies are 4 genes long. Orchid, sand bloom, and rose gene sets are usually two genes long or at most three I think. So on lilies it's often impossible to know the exact splice point in some cases. Ariella: If you're super curious you can make solvents and take a look at your new little genome after you split two more off to find exactly where it spliced and if it has an added (doubled) or subtracted gene. Ariella: End of class Pascalito: I hope you can all uproot flowers ? Dreasimy: but of course :D Pascalito: Try same things with second cross (anyone got a giantx2 ?), you can use my "homework" to assist you Ariella: Or if you want, throw up a chat with Pascalito or me to go over interpreting your flower. Pascalito: Dreasimy, tell me when done on the page, then i'll add my text at bottom Dreasimy: okie AliceBlue: is nexct class same time next week? Ariella: We will be putting all the possbile child lily genomes of both crosses and their color schemes on the GOO guide wiki at the bottom of this saved chat so you can help compare your flower. Ariella: Yes same time next week :) And new homework with orchids! Pascalito: And remember this was the hardest and longest lesson by far! AliceBlue: excellent ty :-) Dreasimy: TY very much Ariella and Pascalito :D Im just working on getting the log up now :D

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NOTE: shades of BLACK show as GREY, you need 4 shades of black for a real black look.

• • Homework-Make one Nut's Essence of Clarity Left Splint/Vampire Right Splint (see Note 1)
  • Homework-Make one Nut's Essence of Fracture Left Splint/Clarity Right Splint.
  • Rename your homework bulbs at any University of Worship (See Note 2 and Note 3).

Clarity Left Splint/Vampire Right Splint: possible hybrid results

We will not takeg shortening or lengthening of the child genome into account at first, this is a good habit.

1. Length of the hybrid : (36 + 12) / 2 = 24

2. Centering parent genomes:

  IRIRIOIOIYIYGORRGRORGRROGORRGRORGRRO
  ............IYIYIOIYIOIO

3. Listing the 13 possible splices (13 = shortest parent genome length + 1)

  IRIRIOIOIYIY
  ............IYIYIOIYIOIO   
  2xIR + 5xIO + 5xIY:
     Outer Stamen = 2 x Black(IR+IO+IY) + 2 x Red(IO+IY) (note: only 2 red shades will show up since a total of 4 shades per base color is the maximmum)

  IRIRIOIOIYIYG
  ............ YIYIOIYIOIO
  2xIR + 5xIO + 4xIY:
     Outer Stamen = 2 x Black(IR+IO+IY) + 2 x Red(IO+IY) (note: only 2 red shades will show up since a total of 4 shades per base color is the maximmum)

  IRIRIOIOIYIYGO
  ............  IYIOIYIOIO
  2xIR + 5xIO + 4xIY:
     Outer Stamen =  2 x Black(IR+IO+IY) + 2 x Red(IO+IY) (note: only 2 red shades will show up since a total of 4 shades per base color is the maximmum)

  IRIRIOIOIYIYGOR
  ............   YIOIYIOIO
  2xIR + 5xIO + 3xIY:
     Outer Stamen = 2 x Black(IR+IO+IY) + 1 x Red(IO+IY) + 1 x Magenta(IO) (note: a total of 4 shades per base color is the maximmum)

  IRIRIOIOIYIYGORR
  ............    IOIYIOIO
  2xIR + 5xIO + 3xIY + 1xGORR:
     Outer Stamen = 2 x Black(IR+IO+IY) + 1 x Red(IO+IY) + 1 x Magenta(IO) (note: a total of 4 shades per base color is the maximmum)
     Outer North Petal Set = 1 x Cyan(GORR)

  IRIRIOIOIYIYGORRG
  ............     OIYIOIO
  2xIR + 4xIO + 3xIY + 1xGORR: 
     Outer Stamen = 2 x Black(IR+IO+IY) + 1 x Red(IO+IY) + 1 x Magenta(IO)
     Outer North Petal Set = 1 x Cyan(GORR)

  IRIRIOIOIYIYGORRGR
  ............      IYIOIO
  2xIR + 4xIO + 3xIY + 1xGORR:
     Outer Stamen = 2 x Black(IR+IO+IY) + 1 x Red(IO+IY) + 1 x Magenta(IO)
     Outer North Petal Set = 1 x Cyan(GORR)

  IRIRIOIOIYIYGORRGRO
  ............       YIOIO
  2xIR + 4xIO + 2xIY + 1xGORR:
     Outer Stamen = 2 x Black(IR+IO+IY) + 2 x Magenta(IO)
     Outer North Petal Set = 1 x Cyan(GORR)

  IRIRIOIOIYIYGORRGROR
  ............        IOIO
  2xIR + 4xIO + 2xIY + 1xGORR + 1xGROR:
     Outer Stamen = 2 x Black(IR+IO+IY) + 2 x Magenta(IO)
     Outer North Petal Set = 1 x Blue(GORR+GROR)

  IRIRIOIOIYIYGORRGRORG
  ............         OIO
  2xIR + 3xIO + 2xIY + 1xGORR + 1xGROR:
     Outer Stamen = 2 x Black(IR+IO+IY) + 1 x Magenta(IO)
     Outer North Petal Set = 1 x Blue(GORR+GROR)

  IRIRIOIOIYIYGORRGRORGR
  ............          IO
  2xIR + 3xIO + 2xIY + 1xGORR + 1xGROR:
     Outer Stamen = 2 x Black(IR+IO+IY) + 1 x Magenta(IO)
     Outer North Petal Set = 1 x Blue(GORR+GROR)

  IRIRIOIOIYIYGORRGRORGRR
  ............           O
  2xIR + 2xIO + 2xIY + 1xGORR + 1xGROR + 1xGRRO:
     Outer Stamen = 2 x Black(IR+IO+IY)
     Outer North Petal Set = 1 x Black(GORR+GROR+GRRO)

  IRIRIOIOIYIYGORRGRORGRRO
  ............
  2xIR + 2xIO + 2xIY + 1xGORR + 1xGROR + 1xGRRO:
     Outer Stamen = 2 x Black(IR+IO+IY)
     Outer North Petal Set = 1 x Black(GORR+GROR+GRRO)

The next two are examples of added (doubled) or subtracted genes that had a visual effect by creating color gene sets not found on either parent. Usually added (doubled) or subtracted genes are not apparent by visual inspection.

Example of a subtraction:

  IRIRIOIOIYIY
  ............ YIYIOIYIOIO
  2xIR + 5xIO + 4xIY:
     Outer Stamen = 2 x Black(IR+IO+IY) + 2 x Red(IO+IY) (note: only 2 red shades will show up since a total of 4 shades per base color is the maximmum)
     Middle Stamen = 1 x Yellow(IYY) There is no way to have this gene set without a subtracted gene at the splice point.

Exampple of an addition (remember it duplicates the gene from left or right side of the splice point):

  IRIRIOIOIYIYGORRGRORGRRR
  ............           O
  2xIR + 2xIO + 2xIY + 1xGORR + 1xGROR + 1xGRRR:
     Outer Stamen = 2 x Black(IR+IO+IY)
     Outer South Petal Set = 1 x Cyan(GRRR) This gene set could not happen without the added (doubled) gene at the splice point.
     Outer North Petal Set = 1 x Blue (Cyan GORR + Magenta GROR)

Fracture Left Splint/Clarity Right Splint: possible hybrid results

We will not takeg shortening or lengthening of the child genome into account at first, this is a good habit.

1. Length of the hybrid : (18 + 36) / 2 = 27

2. Centering parent genomes:

           ROYGROYGYORGORGOOO.........
  IRIRIOIOIYIYGORRGRORGRROGORRGRORGRRO

3. Listing the 19 possible splices (19 = shortest parent genome length + 1), using the same abbreviated indicators as on the Genomes page

  ROYGROYGYORGORGOOO.........
                    RGRORGRRO
  2xGiant(ROYG) + 1xDwarf(GYOR) + Mag1OSP(GOOO) + Red1ONP=[Mag1ONP(GROR)+Yel1ONP(GRRO)]

  ROYGROYGYORGORGOO .........
                   RRGRORGRRO
  2xGiant(ROYG) + 1xDwarf(GYOR) + Red1ONP=[Mag1ONP(GROR)+Yel1ONP(GRRO)]

  ROYGROYGYORGORGO  .........
                  ORRGRORGRRO
  2xGiant(ROYG) + 1xDwarf(GYOR) + Red1ONP=[Mag1ONP(GROR) + Yel1ONP(GRRO)] 

  ROYGROYGYORGORG   .........
                 GORRGRORGRRO
  2xGiant(ROYG) + 1xDwarf(GYOR) + Black1ONP=[Cyan1ONP(GORR)+Mag1ONP(GROR)+Yel1ONP(GRRO)] 

  ROYGROYGYORGOR    .........
                OGORRGRORGRRO
  2xGiant(ROYG) + 1xDwarf(GYOR) + Black1ONP=[Cyan1ONP(GORR)+Mag1ONP(GROR)+Yel1ONP(GRRO)]  

  ROYGROYGYORGO     .........
               ROGORRGRORGRRO
  2xGiant(ROYG) + 1xDwarf(GYOR) + Black1ONP=[Cyan1ONP(GORR)+Mag1ONP(GROR)+Yel1ONP(GRRO)] 

  ROYGROYGYORG      .........
              RROGORRGRORGRRO
  2xGiant(ROYG) + 1xDwarf(GYOR) + Black1ONP=[Cyan1ONP(GORR)+Mag1ONP(GROR)+Yel1ONP(GRRO)] 

  ROYGROYGYOR       .........
             GRROGORRGRORGRRO
  2xGiant(ROYG) + 1xDwarf(GYOR) + Black1+Yel1ONP=[Cyan1ONP(GORR)+Mag1ONP(GROR)+Yel2ONP(GRRO)]

  ROYGROYGYO        .........
            RGRROGORRGRORGRRO
  2xGiant(ROYG) + 1xDwarf(GYOR) + Black1+Yel1ONP=[Cyan1ONP(GORR)+Mag1ONP(GROR)+Yel2ONP(GRRO)] 

  ROYGROYGY         .........
           ORGRROGORRGRORGRRO
  2xGiant(ROYG) + 1xDwarf(GYOR) + Black1+Yel1ONP=[Cyan1ONP(GORR)+Mag1ONP(GROR)+Yel2ONP(GRRO)] 

  ROYGROYG          .........
          RORGRROGORRGRORGRRO
  2xGiant(ROYG) + Black1+Red1ONP=[Cyan1ONP(GORR)+Mag2ONP(GROR)+Yel2ONP(GRRO)] 

  ROYGROY           .........
         GRORGRROGORRGRORGRRO
  2xGiant(ROYG) + Black1+Red1ONP=[Cyan1ONP(GORR)+Mag2ONP(GROR)+Yel2ONP(GRRO)] 

  ROYGRO            .........
        RGRORGRROGORRGRORGRRO
  1xGiant(ROYG) + Black1+Red1+Mag1ONP=[Cyan1ONP(GORR)+Mag3ONP(GROR)+Yel2ONP(GRRO)]

  ROYGR             .........
       RRGRORGRROGORRGRORGRRO
  1xGiant(ROYG) + Cyan1OSP(GRRR) + Black1+Red1ONP=[Cyan1ONP(GORR)+Mag2ONP(GROR)+Yel2ONP(GRRO)]  

  ROYG              .........
      ORRGRORGRROGORRGRORGRRO
  1xGiant(ROYG) + Black2ONP=[Cyan2ONP(GORR)+Mag2ONP(GROR)+Yel2ONP(GRRO)]

  ROY               .........
     GORRGRORGRROGORRGRORGRRO
  1xGiant(ROYG) + Black2ONP=[Cyan2ONP(GORR)+Mag2ONP(GROR)+Yel2ONP(GRRO)] 

  RO                .........
    YGORRGRORGRROGORRGRORGRRO
  1xGiant(ROYG) + Black2ONP=[Cyan2ONP(GORR)+Mag2ONP(GROR)+Yel2ONP(GRRO)]

  R                 .........
   IYGORRGRORGRROGORRGRORGRRO
  1xYelOS(IY) + Black2ONP=[Cyan2ONP(GORR)+Mag2ONP(GROR)+Yel2ONP(GRRO)] 

                    .........
  YIYGORRGRORGRROGORRGRORGRRO
  1xYelOS(IY) + Black2ONP=[Cyan2ONP(GORR)+Mag2ONP(GROR)+Yel2ONP(GRRO)] 

If you do not find the looks of your cross in this list, you could have an added or subtracted gene at the splice point!

Example of a subtraction:

  ROYGROY           .........
          RORGRROGORRGRORGRRO
  1xGiant(ROYG) + Black1ONP=[Cyan1ONP(GORR)+Mag2ONP(GROR)+Yel2ONP(GRRO)]

Exampple of an addition (remember it duplicates the gene from left or right side of the splice point):

  ROYGROYGYORG      .........
             RRROGORRGRORGRRO
  2xGiant(ROYG) + 1xDwarf(GYOR) + Cyan1OSP(GRRR) + Black1+Yel1ONP=[Cyan1ONP(GORR)+Mag1ONP(GROR)+Yel2ONP(GRRO)]