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Difference between revisions of "Genome Research Roundtable/BlueMiddleStamen"
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Starting with one Silken, two Vampires, and one Crown. | Starting with one Silken, two Vampires, and one Crown. | ||
− | '''Mutation 1:''' Pure Vampire Left, Pure Silken Right using Calico, Green, Prickly | + | '''Mutation 1:''' Pure Vampire Left (#15), Pure Silken Right (#16) using Calico, Green, Prickly |
− | 1Silken: '''I'''RROOOYYYGGGUUUIII | + | 1Silken (#16): '''I'''RROOOYYYGGGUUUIII |
− | 1Vampire: IY'''R'''YIOIYIOIO | + | 1Vampire (#15): IY'''R'''YIOIYIOIO |
− | '''Mutation 2:''' Single mutation Silken Left, Pure Vampire Right using Hairy,Smelly,Striped | + | '''Mutation 2:''' Single mutation Silken Left (#17), Pure Vampire Right (#18) using Hairy,Smelly,Striped |
− | 2Silken: IRR'''I'''OOYYYGGGUUUIII | + | 2Silken (#17): IRR'''I'''OOYYYGGGUUUIII |
− | 1Vampire: IY'''R'''YIOIYIOIO | + | 1Vampire (#18): IY'''R'''YIOIYIOIO |
− | '''Mutation 3:''' Double mutation Silken Left, Pure Crown Right using Hairy, Reticulated, Smelly | + | '''Mutation 3:''' Double mutation Silken Left (#19), Pure Crown Right (#20) using Hairy, Reticulated, Smelly |
− | 3Silken: IRRI'''R'''OYYYGGGUUUIII | + | 3Silken (#19): IRRI'''R'''OYYYGGGUUUIII |
− | 1Crown: UROOU'''O'''OOUROOVURVOOVURVORVURORURORUROR | + | 1Crown (#20): UROOU'''O'''OOUROOVURVOOVURVORVURORURORUROR |
'''Mutation 4:''' Triple mutation Silken Left, Single mutation Crown Right using Phos., Spongy, Spotted | '''Mutation 4:''' Triple mutation Silken Left, Single mutation Crown Right using Phos., Spongy, Spotted | ||
− | 4Silken: IRRIR'''R'''YYYGGGUUUIII | + | 4Silken (#21): IRRIR'''R'''YYYGGGUUUIII |
− | 2Crown: UROOUOOOUROOVURVOOVURVORVURO'''O'''URORUROR | + | 2Crown (#22): UROOUOOOUROOVURVOOVURVORVURO'''O'''URORUROR |
Revision as of 00:21, 4 September 2012
A plan of action for creating a flower with a blue middle stamen using four known mutagens and a high probability crossbreed.
Mutations
First, we will transform the first six genes of Silken to IRRIRR using four mutagens:
Calico, Green, Prickly: Silken on Right, Vampire on Left gives needed I on gene 1
Hairy,Smelly,Striped: Silken on Left, Vampire on Right gives needed I on gene 4
Hairy, Reticulated, Smelly: Silken on Left, Blush/Crown/Silken on Right gives needed R on gene 5
Phos., Spongy, Spotted: Silken on Left, Clarity/Crown/Fracture on Right gives needed R on gene 6
Cross
Now, cross the mutated Silken on Left, Blush on Right
....IRRIRRYYYGGGUUUIII
GRORVGOOOVUROOVIOVIOOVIOOO
A cross which hits (counting on silken) after the 6th gene and before the 15th gene yields a success, unless a gene is deleted before the 6th or after the 15th gene. That's approximately an 8/14, or more than 50% chance of success.
The actual experiment
Starting with one Silken, two Vampires, and one Crown.
Mutation 1: Pure Vampire Left (#15), Pure Silken Right (#16) using Calico, Green, Prickly
1Silken (#16): IRROOOYYYGGGUUUIII
1Vampire (#15): IYRYIOIYIOIO
Mutation 2: Single mutation Silken Left (#17), Pure Vampire Right (#18) using Hairy,Smelly,Striped
2Silken (#17): IRRIOOYYYGGGUUUIII
1Vampire (#18): IYRYIOIYIOIO
Mutation 3: Double mutation Silken Left (#19), Pure Crown Right (#20) using Hairy, Reticulated, Smelly
3Silken (#19): IRRIROYYYGGGUUUIII
1Crown (#20): UROOUOOOUROOVURVOOVURVORVURORURORUROR
Mutation 4: Triple mutation Silken Left, Single mutation Crown Right using Phos., Spongy, Spotted
4Silken (#21): IRRIRRYYYGGGUUUIII
2Crown (#22): UROOUOOOUROOVURVOOVURVORVUROOURORUROR