OE01

Crackly, Dry, Mottled

This page serves as a repository of research notes on Toxin OE01, the first mutagen unlocked in Old Egypt by Renard. It uses 6 Crackly, Dry, Mottled (Negative to Phosphorescent, Prickly, Spotted) moss 5 Cat nip, 6 Hairy Tooth, 4 Nefertari Crown, 6 Salt Water Fungus, 6 Slave Bread mushrooms.

First mutation test: L-Vampire R-Silken

The left splint has an even more vivid color on the outer stamen, Looks like Magenta-4 Yellow 2, so one of the Ys has likely been replaced by an O. No visible effect on silken, which is entirely as expected if it's putting a Y in the 4-6 range of that lily.

Second mutation test: L-Silken R-Vampire

On the left splint a very faint yellow color is visible on the whole stamen. Gene 6 (O) must have been replace by an I.

On the right splint, the color of the stamen is Yellow 2 Magenta 3, so one of the IY alleles was disrupted. From the results above, we expect the genes affected on Vampire to be in the range of 3-4 leaving the results as Right-Vampire 3 (I)

Next Step

The second test allows us to clarify the results of the first. Since the left mutation hits gene 6 (33.3%) on a Silken, that equates to gene 4 on a Vampire. This is consistent with the results seen here, so Left-Vampire 4(Y). The results from the second test let us narrow down the result on the right just a little bit since it eliminates 6 as a possibility so Right-Silken 4-5(O)

Additionally, Left-Silken 6 (O) and Right-Vampire 3(I)

As with my first set of tests (on RP06), my goal at this point is to see the results of this mutagen on a larger genome which will allow us to narrow down the range significantly. Clarity is a useful flower for this purpose since its genome length is 36.

From the first test, on the left splint the mutagen will strike between 10-14. The first 16 genes in Clarity are: IRIRIOIOIYIYGORR

Putting an R or an O into this range would be informative.

If the 10th or 12th genes are hit, the stamen color will lose a yellow and add a cyan or magenta. If the 11th gene is hit, the stamen will lose a yellow If the 13th gene is hit, the leaves will lose color If the 14th gene is hit, an O will cause no change, and an R will cause the formation of a GRRR allele, Cyan South.

So, what flowers have Os and Rs in the region where the right splint is expected to hit (16.667-33.333%)?

Crown has a single U and one V in the 7-13 gene range. Blush has a V, G, and three Os. Both might be informative, but Crown has better chance of being informative. (5/7)>(3/5), so we'll go for that.

References

Genomes for reference to genomes.

Flower_Genome_Theories for interpretation of gene sets.

Mutagen_Research_and_Recipes for information on Mutagen recipes, and location for results to be loaded to.