OE01

Crackly, Dry, Mottled

This page serves as a repository of research notes on Toxin OE01, the first mutagen unlocked in Old Egypt by Renard. It uses 6 Crackly, Dry, Mottled (Negative to Phosphorescent, Prickly, Spotted) moss 5 Cat nip, 6 Hairy Tooth, 4 Nefertari Crown, 6 Salt Water Fungus, 6 Slave Bread mushrooms.

First mutation test: L-Vampire R-Silken

The left splint has an even more vivid color on the outer stamen, Looks like Magenta-4 Yellow 2, so one of the Ys has likely been replaced by an O. No visible effect on silken, which is entirely as expected if it's putting a Y in the 4-6 range of that lily.

Second mutation test: L-Silken R-Vampire

On the left splint a very faint yellow color is visible on the whole stamen. Gene 6 (O) must have been replace by an I.

On the right splint, the color of the stamen is Yellow 2 Magenta 3, so one of the IY alleles was disrupted. From the results above, we expect the genes affected on Vampire to be in the range of 3-4 leaving the results as Right-Vampire 3 (I)

Next Step

The second test allows us to clarify the results of the first. Since the left mutation hits gene 6 (33.3%) on a Silken, that equates to gene 4 on a Vampire. This is consistent with the results seen here, so Left-Vampire 4(Y). The results from the second test let us narrow down the result on the right just a little bit since it eliminates 6 as a possibility so Right-Silken 4-5(O)

Additionally, Left-Silken 6 (O) and Right-Vampire 3(I)

As with my first set of tests (on RP06), my goal at this point is to see the results of this mutagen on a larger genome which will allow us to narrow down the range significantly. Clarity is a useful flower for this purpose since its genome length is 36.

From the first test, on the left splint the mutagen will strike between 10-14. The first 16 genes in Clarity are: IRIRIOIOIYIYGORR

Putting an R or an O into this range would be informative.

If the 10th or 12th genes are hit, the stamen color will lose a yellow and add a cyan or magenta
If the 11th gene is hit, the stamen will lose a yellow
If the 13th gene is hit, the leaves will lose color
If the 14th gene is hit, an O will cause no change, and an R will cause the formation of a GRRR allele, Cyan South

So, what flowers have Os and Rs in the region where the right splint is expected to hit (16.667-33.333%)?

Crown has a single U and one V in the 7-13 gene range. Blush has a V, G, and three Os. Both might be informative, but Crown has better chance of being informative. (5/7)>(3/5), so we'll go for that.

Third mutation test: L-Clarity R-Crown

A yellow was removed from the clarity stamen, but no color has been added.

On Crown, a UROO has disrupted.

Results have been narrowed, but are not yet conclusive. The range for the gene mutated on Clarity is now limited to 10 (Y), 11 (I), or 12 (Y).

If the 11th gene on Clarity was hit, then it must have been the 8th gene of Right-Crown affected since that is the only possibility which affect the petals without adding color to the stamen. If it was the 10th or 12th gene hit on the Clarity, the range could still be anywhere from 7-12 on the Crown.

Fourth mutation test: L-Crown R-Clarity

On the Crown, once again a UROO allele has been disrupted with no other visible effect.

On the Clarity, an IY set has been disrupted, but magenta can be seen down to the middle stamen. The only way this could have happened is if an IOIY has been changed to IOOY. A very specific change! Clarity's 9th gene (I) has been replace by an O. No color is visible on the Crown stamen, so where through genes 11-15 can an I replace an O, disrupting a UROO petal but not affecting the stamen? Only at the END of a UROO allele. Left-Crown 12(O) Right-Clarity 9(I)

Next Step

The fourth test allows us to narrow down the results of the third test. The range of the left splint has been reduced to 11-13 on Clarity, so 10(Y) has been eliminated. The range of the right splint has been reduced to 9-11. This indicates that gene 8 was NOT affected on the crown, so Clarity 11(I) has been eliminated. Of genes 9-11 on Crown, only gene 9 (U) could replace that Y without having a visible effect on the Stamen. Hence left-Clarity 12(Y) Right-Crown 9(U). Since the gene sizes of Clarity and Crown are nearly identical, we would expect that the left and right genes hit would remain the same when we swap Clarity and Crown, which is exactly what is observed.

Can we narrow it down to a single gene on Dusk yet? On the left splint, the range hit is anywhere from genes 23-26. On the right splint, the range hit is anywhere from genes 17-20.

The first 26 genes of dusk are: GROR GORR UYYY GYOR IY GRRO IYYY

If I could put an R or an I in the range of 17-20, each possibility has a unique and readily identifiable effect.

Genes R and O would have a uniquely identifiable effect in genes 23-26.

On Blush, the left splint would affect 6-8, right would hit 8-10. Genes 6-10 are GOOOV This could be informative, let's get to it.

Fifth mutation test: L-Dusk R-Blush

On the Dusk, Yellow has been removed from all stamen. Gene 23 or 24 were hit. On the Blush, the Outer-South petal has lost color, as expected and Magenta has spread down the stem. An IOOO must have been formed which has only a single explanation!. Left-Dusk 23 (I) Right Blush-6 (G)

Sixth mutation test: L-Blush R-Dusk

On the right, Dusk is now a normal sized flower instead of a semi-dwarf. Breaking the dwarf allele wouldn't do this, since that would just make dusk a giant flower because there's still a giant set there. However, two giants will cancel out a dwarf to make a normal size flower. That could only happen if the first I in the Dusk genome were replace by an O. Right-Dusk 17(I)

Now that we know an I has been transferred to Blush and replaced an O, but the stamen hasn't changed at all. Therefore it must have been the last O of the disrupted GOOO allele. Left-Blush 9(O).

Final Tests

That leaves us with Delicate (16), Energy (42), Fracture (18), and Morning (44) to characterize. Testing Fracture isn't strictly necessary since it has a gene length identical to Silken, so we know exactly where the toxin will mutate that lily.

For Delicate, on the left split the toxin will mutate either the 5th or 6th gene. On the right split, it will mutate the 4th gene.

For Energy, on the left splint the toxin will mutate either the 13th or 14th gene. On the right splint, it will mutate the 9th or 10th gene.

For Morning, on the left splint the toxin will mutate either the 14th or 15th gene. On the right splint, it will mutate the 10th or 11th gene. All of these genes are a Y!

The first 6 genes of Delicate: IOIOIO

The first 17 genes of Energy: VVVVVUYYYUYYYGYYY

The first 16 genes of Morning: IRRRIYYYIYYYUYYY

For Morning, an I would be ideal, which would work with a Dusk double cross since the donated I will allow us to see exactly which gene was hit on both the left and right cross.

On Energy, the difference between a mutation on the 13th and 14th gene is clear based on which petal set is affected. Double crossing with Delicate would likely be informative.

Seventh mutation test: L-Morning R-Dusk

Color extends to middle stamen, so Left-Morning 14 (Y)

Eight mutation test: L-Dusk R-Morning

Outer stamen only: Right-Morning 11 (Y)

Additionally, this result eliminates Right-Silken 4(O) as a possibility, leaving us with Right-Silken 5(O)

Ninth mutation test: L-Energy R-Delicate

Color is missing from the outer south on Energy, and no color is visible in the stamen. A GYYY allele was broken, and not replace with an I. Left-Energy 14 (G) Right-Delicate 4(O)

Tenth mutation test: L-Delicate R-Energy

Magenta has been removed from the Delicate outer stamen and Yellow is visible on the entire stamen of Energy. Left-Delicate 5(I) Right-Energy 10(U)

Eleventh mutation test: L-Crimson R-Crimson

The Crimson lily genome has recently been approved for testing, so this test will be a quick check on whether a mutation on two of those lilies can be readily interpreted.

Left splint should hit gene 33 or 34
Right splint should hit gene 25
Genes 23-34: IY IY UOOO UYYY

On the left, an Inner East has lost some Yellow color, but the stamen remains unchanged. This is consistent with the third Y of UYYY being replaced with an I.

On the right, the outer stamen seems to have lost some yellow, but the middle and inner stamen seem to have gained yellow. This is consistent with Y being added to gene 5, replacing the I there.

Both tests seem to confirm:
Left-Crimson 34(Y)
Right-Crimson 25(I)

References

Genomes for reference to genomes.

Flower_Genome_Theories for interpretation of gene sets.

Mutagen_Research_and_Recipes for information on Mutagen recipes, and location for results to be loaded to.