OE01

Crackly, Dry, Mottled

This page serves as a repository of research notes on Toxin OE01, the first mutagen unlocked in Old Egypt by Renard. It uses 6 Crackly, Dry, Mottled (Negative to Phosphorescent, Prickly, Spotted) moss 5 Cat nip, 6 Hairy Tooth, 4 Nefertari Crown, 6 Salt Water Fungus, 6 Slave Bread mushrooms.

First mutation test: L-Vampire R-Silken

The left splint has an even more vivid color on the outer stamen, Looks like Magenta-4 Yellow 2, so one of the Ys has likely been replaced by an O. No visible effect on silken, which is entirely as expected if it's putting a Y in the 4-6 range of that lily.

Second mutation test: L-Silken R-Vampire

On the left splint a very faint yellow color is visible on the whole stamen. Gene 6 (O) must have been replace by an I.

On the right splint, the color of the stamen is Yellow 2 Magenta 3, so one of the IY alleles was disrupted. From the results above, we expect the genes affected on Vampire to be in the range of 3-4 leaving the results as Right-Vampire 3 (I)

Next Step

The second test allows us to clarify the results of the first. Since the left mutation hits gene 6 (33.3%) on a Silken, that equates to gene 4 on a Vampire. This is consistent with the results seen here, so Left-Vampire 4(Y). The results from the second test let us narrow down the result on the right just a little bit since it eliminates 6 as a possibility so Right-Silken 4-5(O)

Additionally, Left-Silken 6 (O) and Right-Vampire 3(I)

As with my first set of tests (on RP06), my goal at this point is to see the results of this mutagen on a larger genome which will allow us to narrow down the range significantly. Clarity is a useful flower for this purpose since its genome length is 36.

From the first test, on the left splint the mutagen will strike between 10-14. The first 16 genes in Clarity are: IRIRIOIOIYIYGORR

Putting an R or an O into this range would be informative.

If the 10th or 12th genes are hit, the stamen color will lose a yellow and add a cyan or magenta
If the 11th gene is hit, the stamen will lose a yellow
If the 13th gene is hit, the leaves will lose color
If the 14th gene is hit, an O will cause no change, and an R will cause the formation of a GRRR allele, Cyan South

So, what flowers have Os and Rs in the region where the right splint is expected to hit (16.667-33.333%)?

Crown has a single U and one V in the 7-13 gene range. Blush has a V, G, and three Os. Both might be informative, but Crown has better chance of being informative. (5/7)>(3/5), so we'll go for that.

Third mutation test: L-Clarity R-Crown

A yellow was removed from the clarity stamen, but no color has been added.

On Crown, a UROO has disrupted.

Results have been narrowed, but are not yet conclusive. The range for the gene mutated on Clarity is now limited to 10 (Y), 11 (I), or 12 (Y).

If the 11th gene on Clarity was hit, then it must have been the 8th gene of Right-Crown affected since that is the only possibility which affect the petals without adding color to the stamen. If it was the 10th or 12th gene hit on the Clarity, the range could still be anywhere from 7-12 on the Crown.

Fourth mutation test: L-Crown R-Clarity

On the Crown, once again a UROO allele has been disrupted with no other visible effect.

On the Clarity, an IY set has been disrupted, but magenta can be seen down to the middle stamen. The only way this could have happened is if an IOIY has been changed to IOOY. A very specific change! Clarity's 9th gene (I) has been replace by an O. No color is visible on the Crown stamen, so where through genes 11-15 can an I replace an O, disrupting a UROO petal but not affecting the stamen? Only at the END of a UROO allele. Left-Crown 12(O) Right-Clarity 9(I)

Next Step

The fourth test allows us to narrow down the results of the third test. The range of the left splint has been reduced to 11-13 on Clarity, so 10(Y) has been eliminated. The range of the right splint has been reduced to 9-11. This indicates that gene 8 was NOT affected on the crown, so Clarity 11(I) has been eliminated. Of genes 9-11 on Crown, only gene 9 (U) could replace that Y without having a visible effect on the Stamen. Hence left-Clarity 12(Y) Right-Crown 9(U). Since the gene sizes of Clarity and Crown are nearly identical, we would expect that the left and right genes hit would remain the same when we swap Clarity and Crown, which is exactly what is observed.

Additionally, this range eliminates gene 5 of Silken as a possibility, so Right-Silken 4(O).

References

Genomes for reference to genomes.

Flower_Genome_Theories for interpretation of gene sets.

Mutagen_Research_and_Recipes for information on Mutagen recipes, and location for results to be loaded to.