RP06

Calico, Green, Prickly

RP06, the sixth mutagen unlocked in River Plains by Renard uses 3 Calico, Green, and Prickly (Negative to Reticulated,Spongy,Spotted) moss 2 duelling serpent,2 dung root, 4 heaven's torrent, 2 pool of tranquility, 3 spiderling mushrooms.

First mutation test: L-Vampire R-Silken

First mutation

The effect on L-Vampire is subtle, slightly bluer than a pure Vampire. Perhaps an IY was disrupted?

The effect on R-Silken is obvious, Cyan1 on the Outer and Middle stamen, requiring an IRR genome.

Only a replacement of the first allele (R) with an I would cause this effect, so RP06 hits the 1/18 of the Right Genome.

Additional information can be found from the second mutation test, which showed that RP06 hits 4/18 of the left Genome. The closest only I on Vampire this range would possibly hit would be allele 3/12.

Second mutation test: L-Silken R-Vampire

Second mutation

The effect on L-Silken is obvious, Magenta1 on the Outer and Middle stamen, requiring an IOO genome.

Only a replacement of the fourth allele (O) with an I would cause this effect, so RP06 hits the 4/18 of the Left Genome.

The effect on R-Vampire is subtle, but clearly an I was hit. Given the information from the first test, it must have hit 1/12.

Next step

Already I've been able to narrow down the allele hit locations quite well, but I'd like to get more precise with a larger genome. Clarity has a generous genome length of 36, and lots of Stamen related alleles (I) toward the beginning of the genome. The mutagen should hit somewhere between the 6th and the 10th allele on Clarity.

The first 10 alleles are as follows: IRIRIOIOIY

I will attempt to add an R into this sequence. If the R is added to the 6th or 8th allele, the outer stamen will become sea green. If the mutagen hits the 7th allele, an IO sequence will be disrupted, removing some magenta colour from the outer stamen creating a pale green. If the mutagen hits the 9th allele, an IY will be disrupted, removing some yellow colour from the outer stamen creating a bluish grey. If the mutagen hits the 10th allele, the IY will become and IR creating a slate blue colour.

For this to work, I need to find a lily with an R allele at the very beginning of the sequence. Choices include Fracture or Silken Fracture will do nicely, it is 18 alleles in length so I know for certain what it will hit since its length matches Silken.

If the fourth test, were be Fracture Left, Clarity Right.

The fourth allele of Fracture, a G will be placed on either the first or second allele of Clairty. Either will look like the same result, which is not desirable.

The fourth test, will be Silken Left, Clarity Right.

The fourth allele of Silken, an O will be placed on either the first or second allele of Clarity. Visually, if the first or third allele is hit, the outer stamen will become a rosy brown. If the second or fourth allele is hit, the middle stamen will be a red-violet since instead of just disrupting an IR, the IR will become an IO.

With such distinctive results on a higher length genome, the target left and right alleles should be narrowed significantly.

Third mutation test: L-Clarity R-Silken

An I was transplanted from the Clarity to the Silken and an IO allele set was disrupted on the Clarity. Given our previous results, allele 7 must have been hit by the mutagen.

Fourth mutation test: L-Silken R-Clarity

No colour is visible on the Silken Stamen, so when the fourth allele was replaced, it was not replaced by an I narrowing down the possible allele's to the second or fourth allele (R). The red-violet color on the stamen now present bears out this theory, as an IR allele set must have been replaced by an IO. Almost certainly the second because if it affected the fourth out of 36 alleles in this case, it would have affected the second out of 18 alleles in Silken so I will rule out allele 4 based on that fact.

Next step

So, where to go from here. Ultimately I would like to characterize this mutagen based on Dusk, since that is the sea lily with the longest genome which is definitively known. On the right splint, this mutagen would hit alleles 2, 3, or 4.

The first four alleles of Dusk are: GROR, a magenta north set. By inserting an I into either of those three positions, a uniquely identifiable result will be formed. In the second, Magenta will be added to the outer stamen. In the third, Cyan will be added to the outer stamen. If the fourth is hit, the I is followed by a G: no effect on the stamen. In any case, the magenta north set will be disrupted.

So, on the right split, Dusk can be characterized perfectly. What about on the left splint? If what we know of the left splint already is accurate, it should only hit allele 13 disrupting the G of a GYOR "Dwarf" genome allele. Ideally this would be replace by a G (lack of mutation is still information) or an I so that the changes to the stamen can either confirm or deny that the correct allele was hit.