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Difference between revisions of "OE05"
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=Second mutation test: L-Silken R-Vampire= | =Second mutation test: L-Silken R-Vampire= | ||
| − | A magenta on the outer stamen has become Cyan. Hence an R was transferred from Silken, so the | + | A magenta on the outer stamen has become Cyan. Hence an R was transferred from Silken, so the left splint affects a gene far on the left. |
=Discussion and next step= | =Discussion and next step= | ||
Since the right splint affects a gene so far to the left, in the first test either an I or a Y was swapped between the lilies. Either the right splint hits to the far right, or just left of center. | Since the right splint affects a gene so far to the left, in the first test either an I or a Y was swapped between the lilies. Either the right splint hits to the far right, or just left of center. | ||
| + | |||
| + | Clarity Fracture next. | ||
| + | |||
| + | Clarity: IRIRIOIOIYIYGORRGRORGRROGORRGRORGRRO | ||
| + | |||
| + | Fracture: ROYGROYGYORGORGOOO | ||
| + | |||
| + | =Third mutation test: L-Clarity R-Fracture= | ||
| + | Clarity's outer stamen has lost a cyan, Fracture's petals have lost color. | ||
| + | |||
| + | Clearly an I or R has been taken from Clarity and placed onto the very right-hand side of Fracture. However, it was not an R transferred. If it had taken the place of the G a GORR would have been formed, which would have put Cyan on the outer North petals. It could not have replaced one of the Os since that would have created a color visible on Clarity's outer stamen. Hence, an I must have been transferred, and it must have taken the place of the very last gene of Fracture since otherwise color would be visible on Fracture's stamen. | ||
| + | |||
| + | Right-Fracture 18(O)<br> | ||
| + | |||
| + | =Fourth mutation test: L-Fracture R-Clarity= | ||
| + | |||
| + | Fracture is now Semi-Dwarf, so one of the giant genes has been modified. Clarity has lost Yellow in the Outer-North petals. | ||
| + | |||
| + | It was not an R transferred from fracture, because that would have either caused no change or made a visible change to the south petals Cyan. It could have been an O transferred and affecting gene 35, and it could have been a Y affecting anything. Further analysis might narrow it down. | ||
| + | |||
| + | =Discussion and next test= | ||
| + | |||
| + | We know from the results above that on the right splint, the following genes are affected:<br> | ||
| + | Right-Silken 18(I)<br> | ||
| + | Right-Vampire 12(O) | ||
| + | |||
| + | Given the results of the first test, an I was transferred from Vampire. The second test limits the possible range to the first two genes, so:<br> | ||
| + | Left-Vampire 1(I) | ||
| + | |||
| + | That limits the genes affected on Silken and Fracture to be the first two. | ||
| + | |||
| + | As established in the fourth test, it was not an R transferred from fracture, so:<br> | ||
| + | Left-Fracture 2(O) | ||
| + | Left-Silken 2(R) | ||
| + | |||
| + | We know an I was transferred from Clarity on the left, so:<br> | ||
| + | Left-Clarity 3(I) | ||
| + | |||
| + | And as discussed in the fourth test:<br> | ||
| + | Right-Clarity 35(R) | ||
| + | |||
| + | Crimson and Morning both have stamen colors on their left, which make them good candidates for testing in that range. | ||
| + | |||
| + | Crimson: GYORI'''OOOI'''OO<br> | ||
| + | Left splint: 6-9 | ||
| + | |||
| + | Morning: IR'''RR'''<br> | ||
| + | Left-splint: 3-4 | ||
| + | |||
| + | Morning: G'''RR'''O<br> | ||
| + | Right-splint: 42-43 | ||
| + | |||
| + | Dusk: GOR'''R UO'''OO<br> | ||
| + | Right splint: 69-71 | ||
| + | |||
| + | L-Crimson, R-Morning will not be particularly informative on the right splint, but results on the left splint should prove useful. | ||
| + | |||
| + | L-Morning, R-Dusk will transfer an R to dusk, and each result has a unique interpretation since UROO is a valid allele. If both petals remain unchanged, I won't know exactly where Morning was affected on the Left splint, but that's a risk I'm willing to take. | ||
| + | |||
| + | =Fifth mutation test: L-Crimson, R-Morning= | ||
| + | |||
| + | =Sixth mutation test: L-Morning, R-Dusk= | ||
Revision as of 23:18, 22 September 2012
Crackly, Prickly, Reticulated
This page serves as a repository of research notes on Toxin OE05, the fifth mutagen unlocked in Old Egypt by Renard. It uses 3 Crackly, Prickly, Reticulated (Negative to Hairy, Slimy, Spotted) moss and 7 Beehive, 2 Heart of Ash, 1 Iron Knot, 2 Toad Skin mushrooms.
First mutation test: L-Vampire R-Silken
No change to either flower.
Second mutation test: L-Silken R-Vampire
A magenta on the outer stamen has become Cyan. Hence an R was transferred from Silken, so the left splint affects a gene far on the left.
Discussion and next step
Since the right splint affects a gene so far to the left, in the first test either an I or a Y was swapped between the lilies. Either the right splint hits to the far right, or just left of center.
Clarity Fracture next.
Clarity: IRIRIOIOIYIYGORRGRORGRROGORRGRORGRRO
Fracture: ROYGROYGYORGORGOOO
Third mutation test: L-Clarity R-Fracture
Clarity's outer stamen has lost a cyan, Fracture's petals have lost color.
Clearly an I or R has been taken from Clarity and placed onto the very right-hand side of Fracture. However, it was not an R transferred. If it had taken the place of the G a GORR would have been formed, which would have put Cyan on the outer North petals. It could not have replaced one of the Os since that would have created a color visible on Clarity's outer stamen. Hence, an I must have been transferred, and it must have taken the place of the very last gene of Fracture since otherwise color would be visible on Fracture's stamen.
Right-Fracture 18(O)
Fourth mutation test: L-Fracture R-Clarity
Fracture is now Semi-Dwarf, so one of the giant genes has been modified. Clarity has lost Yellow in the Outer-North petals.
It was not an R transferred from fracture, because that would have either caused no change or made a visible change to the south petals Cyan. It could have been an O transferred and affecting gene 35, and it could have been a Y affecting anything. Further analysis might narrow it down.
Discussion and next test
We know from the results above that on the right splint, the following genes are affected:
Right-Silken 18(I)
Right-Vampire 12(O)
Given the results of the first test, an I was transferred from Vampire. The second test limits the possible range to the first two genes, so:
Left-Vampire 1(I)
That limits the genes affected on Silken and Fracture to be the first two.
As established in the fourth test, it was not an R transferred from fracture, so:
Left-Fracture 2(O)
Left-Silken 2(R)
We know an I was transferred from Clarity on the left, so:
Left-Clarity 3(I)
And as discussed in the fourth test:
Right-Clarity 35(R)
Crimson and Morning both have stamen colors on their left, which make them good candidates for testing in that range.
Crimson: GYORIOOOIOO
Left splint: 6-9
Morning: IRRR
Left-splint: 3-4
Morning: GRRO
Right-splint: 42-43
Dusk: GORR UOOO
Right splint: 69-71
L-Crimson, R-Morning will not be particularly informative on the right splint, but results on the left splint should prove useful.
L-Morning, R-Dusk will transfer an R to dusk, and each result has a unique interpretation since UROO is a valid allele. If both petals remain unchanged, I won't know exactly where Morning was affected on the Left splint, but that's a risk I'm willing to take.
