OE07

Calico, Dry, Smelly

This page serves as a repository of research notes on Toxin OE07, the seventh mutagen unlocked in Old Egypt by Renard. It uses 6 Calico, Dry, Smelly (Negative to Fuzzy Mottled Slimy) moss and 5 Bleeding Hand, 7 Colt Foot, 6 Nefertari's Crown, 2 Pool of Tranquility, 6 Scorpion Brood mushrooms.

First mutation test: L-Vampire R-Silken

The Vampire outer stamen has lost some magenta, no change is visible on the Silken.

Second mutation test: L-Silken R-Vampire

No change is visible on the silken, the vampire outer stamen has had an IO (Magenta) allele replaced with IY (Yellow). Hence, on the left splint, the affected gene is somewhat left of center.

Discussion and next step

The second test is enough to indicate that either gene 5 or gene 6 was affected on the left splint in the first round of tests. The right splint is still ambiguous.

Not much to go on yet, so that brings us to the standby: Delicate-Fracture double cross.

Third mutation test: L-Delicate R-Fracture

Delicate has lost some yellow from its outer stamen, and Fracture has lost the color from its leaves. The right splint is now known to affect a gene rather far to the right.

Fourth mutation test: L-Fracture R-Delicate

Fracture is now double giant, and Delicate is normal size. We know from previous tests that on the left splint, the gene affected is left of center. Since the giant was disrupted but not one of the dwarf alleles, we know that gene 9 must have been affected. This allows us to identify the left gene affected on all lilies.

Left-Fracture 9(Y)
Left-Silken 9(Y)
Left-Vampire 6(O)
Left-Delicate 8(Y)

Discussion and next step

Knowing that the right splint hits so far to the right, and a Y is taken from Silken on the left splint tells us that an I must have been removed from Vampire.

Right-Vampire 11(I)

That restricts the range on Delicate to either gene 14 (Y) or 15 (O). Since a Y was taken from Fracture on the left splint, that leaves us with:

Right-Delicate 15(O)

Which narrows us down to either gene 16 or 17 on Fracture and Silken.

Where does that lead us next?

Morning: IRRR IYYY IYYY UYYY UYYY UROR UROR GRRR GYYY GORR GRRO
Left Splint: 20-22
Right Splint: 39-41
That's a fairly useful range, even with many possible different genes, we can generally readily establish which petal was affected which helps us narrow down gene ranges quite well.

Clarity: IRIR IOIO IYIY GORR GROR GRRO GORR GROR GRRO
Left Splint: 17-18
Right Splint: 32-33
Left-Morning Right-Clarity looks very informative. Every possible result has a unique interpretation.
Left-Clarity Right-Morning looks much less useful, so we'll stick to one test for now.

Fifth mutation test: L-Morning R-Clarity

Morning has lost Yellow-West, Clarity has lost Magenta-North.
Left-Morning 21(U)
Right-Clarity 32(R)

Discussion and next step

With the range now significantly narrowed, can we work with Dusk or Crimson?

Dusk: GROR GORR UYYY GYOR IY GRRO IYYY GRRO GORR GROR ROYG IO GROR IOOO IYY IY URRR GRRO GORR UOOO
Left splint: 34-35
Right splint: 64-65
The left splint should be easy to test.

Crimson (Starting from gene 47): UROR GOOO GOOO GOOO GYYY GYYY GYYY GROR GROR GROR GROR GRRO GRRO GRRO GRRO
Left splint: 49-51
Right splint: 93-95
Not very promising.

Energy: Energy VVVVV UYYY UYYY GYYY GYYY GRRO GRRO UROR UROR VVVVV
Left splint: 20
Right splint: 37-38
Left-Dusk Right-Energy would work just fine.

Sixth mutation test: L-Dusk R-Energy

Dusk has lost North-Magenta, Energy has lost West-Yellow
Left-Dusk 35(G)
Right-Energy 37(R)

Discussion and next step

Progress has been made on narrowing the splint ranges. Back to Crimson:

Crimson (Starting from gene 47): UROR GOOO GOOO GOOO GYYY GYYY GYYY GROR GROR GROR GROR GRRO GRRO GRRO GRRO
Left splint: 50-51
Right splint: 93-94
That's much easier to work with, we can even cross Crimson with itself and know at least what gene was affected on the left splint.

Seventh mutation test: L-Crimson R-Crimson

No change to either flower.
Left-Crimson 50 R
Right-Crimson 93 R

That narrows down the ranges enough that testing is complete.

References

Genomes for reference to genomes.

Flower_Genome_Theories for interpretation of gene sets.

Mutagen_Research_and_Recipes for information on Mutagen recipes, and location for results to be loaded to.