User:Rania/workshop

RECIPES

• A Mutagen SWAPS one gene from each plant being crossed

Sea Lily

WE are going to make a Crown have Magenta shade 3 Inner West Petal and a Cyan shade 3 Inner East Petal for our first one.

Crown

TO To do This we need to change the UROR on Crown to URRR

• Crown Genome: (Length 37)

1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37
U	R	O	O	U	R	O	O	U	R	O	O	V	U	R	V	O	O	V	U	R	V	O	R	V	U	R	O	R	U	R	O	R	U	R	O	R

As you can see the 28th gene, the 32nd gene and the 36th genes (in bold print) need to be changed.

• We will be Changing the 28th gene first in this recipe. First we need to know the splint ranges that will move the 28th gene.
  • To determine that you have two options. Option 1 is do the math
    • Formula for Minimum Range is:
      • (X-1) / L * 100% (Note: X=gene# L=Genome Length)
    • Formula for Maximum Range is:
      • X / L * 100% (Note: X=Gene# L=Genome Length)
  • Option 2 is use the splint range chart found on GRRT Sealilies page: http://www.atitd.org/wiki/tale7/GRRT_Sealilies_Gene-Ranges#Sea_Lily_Genome_and_Gene_Ranges
• We have found that to move the 28th we need a Mutagen that has splint ranges between 72.97% - 75.68%.
  • Now we go to http://www.atitd.org/wiki/tale7/GRRT_Mutagen_Research_and_Recipes#MUTAGENS_Splint_Ranges and find the mutagen has the splint ranges between 72.97% - 75.68%