OE02

Green, Prickly, Spongy

This page serves as a repository of research notes on Toxin OE02, the second mutagen unlocked in Old Egypt by Renard. It uses 1 Green, Prickly, Spongy (Negative to Reticulated, Smelly, Striped) moss 3 Peasant Foot, 6 Golden Sun mushrooms.

First mutation test: L-Vampire R-Silken

An IY allele has been broken on Vampire, and replaced by an IR. No change is visible on the Silken Since the R was taken from the right splint, the toxin hits the first three Right-Silken 1-3 (R). No color is visible on the Silken stamen, so it was a Y taken from Vampire, not an I meaning on the left it hits gene 2, 4, or 8.

Second mutation test: L-Silken R-Vampire

No change is visible on the silken, An IY allele has been broken on Vampire, but without knowing more details about what is affected on the left splint it's difficult to narrow down further.

Next step

Not much to go on yet. There is still a pretty broad range on what gene has been affected on the left. While still facing so many unknowns, a useful double cross is Delicate-Fracture. Both have few genes with noticeable effect, and if we can determine exactly what gene is affected on the Fracture it will tell us what was affected on Silken too since they both have the same number of genes.

Third mutation test: L-Delicate R-Fracture

The Delicate has lost some Yellow in the outer stem, replace by Cyan so an IY has been replaced by an IR. Fracture is now Semi-Dwarf, so a Giant gene was broken and the R was replayed by a Y from Delicate. For Left-Delicate gene 8, 10, or 12 was affected.

The giant set is ROYG, so it must be Right Fracture 1-(R)

Fourth mutation test: L-Fracture R-Delicate

Fracture is now Double-Giant, biggest lily I've ever mutated! The Dwarf gene must have been broken. On the right, Delicate has lost an IO, and it has not been replaced by anything.

Therefore the hit is somewhere between genes 8-11. It cannot be 8, because if that gene were replaced, that would break both the dwarf and a giant, and the Fracture wouldn't be quite so large. Since the next three possibilities are all color genes, it must have been an I which was transferred from Delicate. No color is visible in the Fracture stamen, so that only leaves Left-Fracture 11(R). Since the first gene of an 18 genome lily was affected by the right-splint of this mutagen, the same would hold for a 16 gene lily. Right-Delicate 1(I)

Discussion and next step

Right-Fracture 1(Y) informs us that this toxin will affect the first gene of any flower with a shorter genome.
Right-Vampire 1(I) Right-Silken 1(R)

This has allowed us to characterize the results of the first two tests significantly. From the third test, on the left split this mutagen clearly hits center-right, so it must be Left-Vampire (8) Y

Since it's Left-Fracture 11(R), we know it must be Left-Silken 11(G).

Since Delicate has a smaller gene size, the left-splint gene affected will be less than or equal to 11, but not much less. Left-Delicate 10 (Y) is the only real option.

Now that the toxin has been fully characterized for all the small genomes, let's see if we can narrow down the range any further with a larger genome. What about a Clarity-Crown double cross? Left splint should hit in the 22-23 range for both, right splint in the 1-3 range.

Left-Crown Right-Clarity should be informative. 22-23 is in a bad range for testing with Crown because there's a lot of Vs and odd letters in the midst of that range. However, there's a high probability of throwing an I in there which would be informative, and if we swap the color gene in stead that is probably going to be visible on Clarity's stamen. Useful.

Left-Clarity Right-Crown looks like it won't be that great. There's a high chance both flowers will come out unchanged because an R will have been swapped between them, but with the results of the fifth test we'll have a very good idea of which genes were affected anywys.

References

Genomes for reference to genomes.

Flower_Genome_Theories for interpretation of gene sets.

Mutagen_Research_and_Recipes for information on Mutagen recipes, and location for results to be loaded to.