OE02

Green, Prickly, Spongy

This page serves as a repository of research notes on Toxin OE02, the second mutagen unlocked in Old Egypt by Renard. It uses 1 Green, Prickly, Spongy (Negative to Reticulated, Smelly, Striped) moss 3 Peasant Foot, 6 Golden Sun mushrooms.

First mutation test: L-Vampire R-Silken

An IY allele has been broken on Vampire, and replaced by an IR. No change is visible on the Silken Since the R was taken from the right splint, the toxin hits the first three Right-Silken 1-3 (R). No color is visible on the Silken stamen, so it was a Y taken from Vampire, not an I meaning on the left it hits gene 2, 4, or 8.

Second mutation test: L-Silken R-Vampire

No change is visible on the silken, An IY allele has been broken on Vampire, but without knowing more details about what is affected on the left splint it's difficult to narrow down further.

Next step

Not much to go on yet. There is still a pretty broad range on what gene has been affected on the left. While still facing so many unknowns, a useful double cross is Delicate-Fracture. Both have few genes with noticeable effect, and if we can determine exactly what gene is affected on the Fracture it will tell us what was affected on Silken too since they both have the same number of genes.

Third mutation test: L-Delicate R-Fracture

The Delicate has lost some Yellow in the outer stem, replace by Cyan so an IY has been replaced by an IR. Fracture is now Semi-Dwarf, so a Giant gene was broken and the R was replayed by a Y from Delicate. For Left-Delicate gene 8, 10, or 12 was affected.

The giant set is ROYG, so it must be Right Fracture 1-(R)

Fourth mutation test: L-Fracture R-Delicate

Fracture is now Double-Giant, biggest lily I've ever mutated! The Dwarf gene must have been broken. On the right, Delicate has lost an IO, and it has not been replaced by anything.

Therefore the hit is somewhere between genes 8-11. It cannot be 8, because if that gene were replaced, that would break both the dwarf and a giant, and the Fracture wouldn't be quite so large. Since the next three possibilities are all color genes, it must have been an I which was transferred from Delicate. No color is visible in the Fracture stamen, so that only leaves Left-Fracture 11(R). Since the first gene of an 18 genome lily was affected by the right-splint of this mutagen, the same would hold for a 16 gene lily. Right-Delicate 1(I)

Discussion and next step

Right-Fracture 1(Y) informs us that this toxin will affect the first gene of any flower with a shorter genome.
Right-Vampire 1(I) Right-Silken 1(R)

This has allowed us to characterize the results of the first two tests significantly. From the third test, on the left split this mutagen clearly hits center-right, so it must be Left-Vampire (8) Y

Since it's Left-Fracture 11(R), we know it must be Left-Silken 11(G).

Since Delicate has a smaller gene size, the left-splint gene affected will be less than or equal to 11, but not much less. Left-Delicate 10 (Y) is the only real option.

Now that the toxin has been fully characterized for all the small genomes, let's see if we can narrow down the range any further with a larger genome. What about a Clarity-Crown double cross? Left splint should hit in the 22-23 range for both, right splint in the 1-3 range.

Left-Crown Right-Clarity should be informative. 22-23 is in a bad range for testing with Crown because there's a lot of Vs and odd letters in the midst of that range. However, there's a high probability of throwing an I in there which would be informative, and if we swap the color gene in stead that is probably going to be visible on Clarity's stamen. Useful.

Left-Clarity Right-Crown looks like it won't be that great. There's a high chance both flowers will come out unchanged because an R will have been swapped between them, but with the results of the fifth test we'll have a very good idea of which genes were affected anywys.

Fifth mutation test: Left-Crown Right-Clarity

A Cyan has been lost from Clarity, and no color is visible on Crown's stamen. This is consistent with Right-Clarity 2(R) and Left-Crown 22(V)

Sixth mutation test: Left-Clarity Right-Crown

No change is visible. This is consistent with a Right-Crown 2(R) replacing another R on clarity. Since the previous tests showed Left-Crown 22, the effect on Clarity cannot affect a higher numbered gene (since clarity has a smaller genome). Hence Left-Clarity 22(R) and Right-Crown (2)R

Next step

Now, can we characterize this mutagen on Dusk?

Dusk: On the left splint, it is expected to affect genes 43-44 (an IO allele). On the right splint, it is expected to affect genes 3-4 (Part of GROR, a magenta North set).

Morning: On the left split, it is expected to affect genes 26-27 (The center genes of UROR). On the right splint, it is expected to affect genes 2-3 (IRRR).

If the petals are unchanged from Left-Morning Right-Dusk some uncertainty might result, but even if that does happen the results of the other test should narrow the range enough to clear even that up.

Seventh mutation test: Left-Dusk Right-Morning

Cyan is missing from the entire stamen of Morning, replaced by a Magenta on top. A magenta in Dusk's outer stamen is now Cyan.

Left-Dusk 44(O)
Right-Morning 2(R)

Eighth mutation test: Left-Morning Right-Dusk

Both are completely unchanged! Either it is Left-Morning 27(O) Right-Dusk 3(O) or Left-Morning 26(R) Right-Dusk 4 (R). Further testing is required.

Final Tests

It is unnecessary to test Energy on the right splint, that's in the middle of a bunch of Vs and no result will have a clear explanation. However, I do want to see what happens to Dusk on the right splint more clearly.

What would the crosses look like?

Left-Energy, Right-Blush: Energy is expected to have the 25th gene mutated, the O of a GRRO allele. On Blush it might be the first or second gene affected, which are the first two genes of a GROR allele. If the G is transferred, both petals lose color. If the R is transferred, Cyan will appear on Energy's south petals.

Left-Blush, Right-Dusk: Blush is expected to have the 16th gene mutated, an I of an IO allele. Replace that with an O or an R and the outer stamen will lose some Magenta. However, the I will transfer to the Dusk, and the color that appears on the outer stamen will specify what gene on Dusk was changed.

These last two tests should completely characterize the toxin for all the standard lilies.

Ninth mutation test: Left-Energy Right-Blush

In both cases, the north petals lost color as expected, but no additional color was apparent on Energy's south petals.

Left-Energy 25(O)
Right-Blush 1(G)

Tenth mutation test: Left-Blush Right-Dusk

Dusk has lost Magenta in the north petal set as expected, and Blush's outer stamen is somewhat paler than it was before. The stamen of Dusk has taken on a sickly hue, implying that Cyan has been added to the genome of the outer stamen. Hence:

Left-Blush 16(I)
Right-Dusk 3(O)

and therefore to conclude test 8:

Left-Morning 27(O)

Eleventh mutation test: Left-Crimson Right-Crimson

The Crimson lily has recently been cleared for testing. It has a very large genome, 106 genes. This will make it quite useful for narrowing down mutation ranges even further. Trusting the listed genes to be accurate, I'll cross Crimson with itself to see if the results can be uniquely interpreted.

Left splint should hit gene 63 or 64
Right splint should hit gene 3, 4, or 5
Genes 1-8: GYORIOOO
Genes 63-66: GYYY

On the right, the dwarf gene has been broken. Gene 5 has been eliminated as a possibility.

On the left, the Outer South petals have lost yellow. This does not narrow down the possibilities any further.

More testing will be required to fully characterize the results of this toxin on Crimson.

Ideally, onto the left splint I would place a U. The outer south yellow petals will still be lost, but if the inner east petal set gains a Yellow it will be clear that gene 63 was affected. Otherwise, 64. No known flowers will supply a U though, but Blush will provide a G. If both flowers are unaffected by the mutagen, the toxin hits gene 63. Otherwise 64.

Onto the right splint, I will seek to add a Y which immediately follows the I from a flower on the left splint. The color change of the flower on the left will inform me of just which gene was transferred. Delicate would be perfect for this task.

Twelfth mutation test: Left-Crimson Right-Blush

No visible change. Left-Crimson 63(G)

Thirteenth mutation test: Left-Delicate Right-Crimson

Yellow has been removed from Delicate's outer stamen, Cyan (R) has been added. Right-Crimson 4(R)

References

Genomes for reference to genomes.

Flower_Genome_Theories for interpretation of gene sets.

Mutagen_Research_and_Recipes for information on Mutagen recipes, and location for results to be loaded to.