OE03

Mottled, Smelly, Spotted

This page serves as a repository of research notes on Toxin OE03, the second mutagen unlocked in Old Egypt by Renard. It uses 3 Mottled, Smelly, Spotted (Negative to Calico,Dry,Slimy) moss and 3 Spiderling mushrooms.

First mutation test: L-Vampire R-Silken

No change to either flower.

Second mutation test: L-Silken R-Vampire

An IO on Vampire is missing. No change is apparent on the silken.

Next step

Not much to go on yet. There is still a pretty broad range on what gene has been affected on the right. While still facing so many unknowns, a useful double cross is Delicate-Fracture. Both have few genes with noticeable effect, and if we can determine exactly what gene is affected on the Fracture it will tell us what was affected on Silken too since they both have the same number of genes.

Third mutation test: L-Delicate R-Fracture

On the left, a yellow has been removed from the outer stamen. This suggests that the gene affected on the left splint is toward the center.

Fracture is now a Semidwarf, so a Giant allele has been broken and the mutagen affects the left part of the genome when the flower is on the right splint.

Fourth mutation test: L-Fracture R-Delicate

On the left, no change is apparent to the Fracture. Genes 12-14, somewhat right of center, are not part of any functional allele of Fracture, so they are a likely target.

On the right, the outer stamen is missing some magenta, further evidence that the gene affected on the right splint is left of center.

Next step

The fact that the affected gene on the right splint is left of center is quite informative. Since an IO allele was broken on Vampire in the second test, that narrows the possible gene range to 5 or 6.

On the left splint, somewhat right of center seems likely. Where does that lead us?

No change is apparent from the first test, so two identical genes were swapped.
On the right splint, starting with the center of Silken's gene and moving left, that eliminates I as a possibility. Ys happen immediately as we move left.
On the left splint, starting from the center and moving right, Vampire only has a single Y gene. It seems to fit, so we'll pencil it in for now, Left-Vampire 8(Y). That would limit the upper range to 66.67%

References

Genomes for reference to genomes.

Flower_Genome_Theories for interpretation of gene sets.

Mutagen_Research_and_Recipes for information on Mutagen recipes, and location for results to be loaded to.