OE03

Mottled, Smelly, Spotted

This page serves as a repository of research notes on Toxin OE03, the third mutagen unlocked in Old Egypt by Renard. It uses 3 Mottled, Smelly, Spotted (Negative to Calico,Dry,Slimy) moss and 3 Spiderling mushrooms.

First mutation test: L-Vampire R-Silken

No change to either flower.

Second mutation test: L-Silken R-Vampire

An IO on Vampire is missing. No change is apparent on the silken.

Next step

Not much to go on yet. There is still a pretty broad range on what gene has been affected on the right. While still facing so many unknowns, a useful double cross is Delicate-Fracture. Both have few genes with noticeable effect, and if we can determine exactly what gene is affected on the Fracture it will tell us what was affected on Silken too since they both have the same number of genes.

Third mutation test: L-Delicate R-Fracture

On the left, a yellow has been removed from the outer stamen, but yellow is now visible all the way into the inner stamen. An IYIY has been replaced with IYYY.

Fracture is now a Semidwarf, so a Giant allele has been broken and the mutagen affects the left part of the genome when the flower is on the right splint.

Fourth mutation test: L-Fracture R-Delicate

On the left, no change is apparent to the Fracture. Genes 12-14, somewhat right of center, are not part of any functional allele of Fracture, so they are a likely target.

On the right, the outer stamen is missing some magenta, further evidence that the gene affected on the right splint is left of center.

Next step

The fact that the affected gene on the right splint is left of center is quite informative. Since an IO allele was broken on Vampire in the second test, that narrows the possible gene range to 5 or 6. That brings the range on the right splint to 33.333-50.000%

We know more about the right splint. On Fracture, one of the first seven genes were effected. This would indicate that the range on the right splint is from 0-38.889%. Therefore the range on the right splint is from 33.333%-38.889%.

That eliminates Right-Vampire 6(O) as a possibility.
Right-Vampire 5(I)

On an 18 genome set like Silken or Fracture, the range on the right has become 7.
Right-Silken 7(Y)
Right-Fracture 7(Y)

On the 16 genome of Delicate, that limits the range on the right splint to 6 or 7. Only 6 is a possibility, since 7 would have resulted in a yellow stamen being disrupted instead of magenta. Right-Delicate 6(O)

Now that we've characterized the right splint, what do we know about the left?

From test 4, an O was taken from Delicate and place on Fracture with no apparent change on Fracture, but Delicate has changed so we know it wasn't simply replaced by another O. 12-14 (GOR) can be replaced without altering Fracture's appearance. It wasn't 13, because that would cause no change to Delicate. It's not 14 since the cyan color would be visible on Delicate's stamen. That leaves us with:
Left-Fracture 12(G)
Left-Silken 12(G)
which does not contradict the results of test 2.

That also limits the range of Vampire to gene 8.
Left-Vampire 8(Y)
Which confirms the results from test 1.

For the 16 gene Delicate, that narrows the left-splint range down to gene 10 or 11, which must be 11 to get the IYYY allele noted earlier.
Left-Delicate 11(I)

Now we can move on to larger genomes. Crown-Clarity double cross!

Clarity (Genes 12-24): GORRGRORGRRO
Left splint genes: 23-24
Right splint genes: 13-14

Crown (Genes 13-25): VURVOOVURVORV
Left-splint genes: 24-25
Right-splint genes: 13-14
This puts is smack dab in the middle of the difficult testing range on Crown. Expect little visible effect on Crown.

Fifth mutation test: L-Crown R-Clarity

No change to crown, outer north petals lose cyan on clarity.

It's not Left-Crown 24 Right-Clarity 14, but that's about all we can say.

Sixth mutation test: L-Clarity R-Crown

No change to Crown, outer north petals lose yellow on Clarity.

Next step

Well that was a wash. What would an Energy-Morning cross do?

Energy (Genes 13-28): GYYYGYYYGRROGRRO
Left-splint genes: 27-28
Right-splint genes: 15-16

Morning (Genes 13-32): UYYYUYYYURORURORGRRR
Left-splint genes: 28-30
Right-splint genes: 15-17

That would be essentially worthless. Looks like we'll need to use some known flowers.

Putting an O on the left splint of morning would be informative.
Gene 28: A UROR is broken, but a UROO is formed, yellow becomes magenta on the west petals.
Gene 29: A GRRR is broken, replaced by ORRR. Cyan lost from outer south.
Gene 30: A GRRR becomes GORR, Cyan outer south becomes Cyan outer north.
Delicate can provide this.

Putting an I on the right splint of morning would be informative based on how far changes to the stamen go. Delicate does this too.

Seventh mutation test: Left-Morning Right-Delicate

Left-Morning 28(R)

Eighth mutation test: Left-Delicate Right-Morning

Right-Morning 15(Y)

Next step

That narrows down the ranges significantly further, and also make conclusions about several other lilies.

Clarity:
Left-Splint 23 R
Right-Splint 13 O

Crown:
Left-Splint 24 R
Right-Splint 13 V

Energy:
Left-Splint 27 R
Right-Splint 15 Y

Go big or go home, what can we say about a Crimson-Dusk double cross?

Crimson (Genes 35-70): UROO UROO UROR UROR GOOO GOOO GOOO GYYY GYYY
Left-splint: 67 or 68 Right-splint: 36 or 37

Dusk GROR GORR UYYY GYOR IY GRRO IYYY GRRO GORR GROR ROYG IO GROR
Left-splint: 46 or 47 Right-splint: 25

Left-splint Crimson, Right-splint Dusk would be informative, though the reverse might have ambiguities even after the results of the first test, so we'll only do one test.

We haven't tested Blush yet, but the range is now narrow enough that we know that the right splint will hit 17(O), part of an IO set. We will know what has happened if no change happens, or the changes to Crimson petals and the Blush outer stamen will be obvious.

Left-Blush Right-Crimson it is.

For the Dusk, we can cross it with an energy and put a Y onto the left splint. This will break the GROR allele, and either return nothing (Gene 47) or add a Dwarf allele (46)

Ninth mutation test: Left Crimson, Right Dusk

Dusk outer stamen is unchanged, but middle and inner have lost yellow. Crimson outer south petals have lost yellow.

Right-Dusk 25(Y)
Left-Crimson 67(G)

Tenth mutation test: Left-Blush, R-Crimson

On Crimson, inner west petals have lost magenta, inner east have gained magenta. UROO has become UOOO, the 36th gene was affected.

Right-Crimson 36(R)

Eleventh mutation test: L-Dusk, R-Energy

No Mini-mini-dusk. Left-Dusk 47(O)

References

Genomes for reference to genomes.

Flower_Genome_Theories for interpretation of gene sets.

Mutagen_Research_and_Recipes for information on Mutagen recipes, and location for results to be loaded to.