User:Avanya

Greetings!

Originally from the Bastet server, I transfered to Tale 5 only a few months ago with some friends. (Now we form the Bastet Hounds Guild). My primary passion in Egypt is glassblowing and glassmaking. I also enjoy the Test of Pyrotechnics, Thought puzzles, and crossbreeding flowers. I am always open to helping people in any way that I can, so please chat me anytime. I reside in Alcyone, at 1748, 2478. It's a short jog north of the chariot. My thought puzzle and Art coordinates can be found in my /info.

Glass Services

I sell most glass products, but I do not use a trading point system. I prefer to work out a trade that is fair and considered what the buyer has available to trade. My products include:

• Thermometers
• Barometers (5k, 6k quality)
• Hookah bowls (will assemble into finished hookah with your 14 canvas)
• Chemistry Calibration kits
• Distillation Coils 7.5k quality
• Wine glasses (sorry 8k quality and down, I need more practice)
• Mirrors (with your 200 silver powder though)

I am also willing to sell sheet glass, glass jars, glass pipes, glass rods, glass knives, glass torches. Bulk orders are subject to approval :)

For questions about getting started making glass, please see my guide: http://www.atitd.org/wiki/tale5/User:Avanya/Glassmaking_guide

Buying

I am currently buying materials needed to continue my work with characterizing mutagens. This includes:

• Mushrooms (Sand Spore, Slaves Bread, Razor's Edge, Peasant Foot, and Salt Water Fungus).
• Cobra Skins (as many as possible)

I am also always happy to purchase

• Silk, Sea Lily Bulbs, and Dung.

Yes Dung. I love Dung. (Test of Pyro should be renamed Test of Tons-of-Dung)

Star Labs

I am hoping to help Egypt make better use of its Portable Star Labs. I see so many of them laying around unused, or used as decorations. They are meant to be used and returned to a university of Art (preferably Sterope). I have a Law Petition circulating to help us use them more effectively. Please remember they are used for opening new Star recipes only...they are NOT part of the Principle or Test of Pyrotechnics!! They are given out in a limited amount, so they are a precious resource. Please see http://www.atitd.org/wiki/tale5/Portable_Star_Lab if you have any questions, or chat me in game.

Mutagenics Research:

I am trying to help characterize the mutagens that are being researched. Specially, I'm trying to determine which gene ranges are swapped from the left and right splints. I won't bore you here with genetics theory, it is all explained much more clearly elsewhere on the wiki. But I would like to make my data available to others that share this interest.

This is my first time doing this, so PLEASE chat me if you think I am doing something wrong. Right now, our guru's of genetics are not playing, so I don't have a good source to verify this with. I welcome all input!!

I am not sure how the mutagen range rounds.

For example, if a mutagen range is that it will swap a gene at 10%. Given a genome of length 16, 10% along is between gene 1 and 2. Does it pick the closer gene (in that case, gene 2) or is it a floor rounding function? (that would truncate the extra %, and round down to gene 1). Or does it pick randomly between the two genes? Knowing this is important! I am assuming here it picks the closer gene, but further tests might reveal the actual rounding method.

UPDATE July 16th, 2011

After scouring past mutagenic notes, its come to my attention that the previous tales mutagenic experts used the ceiling function as their rounding method. I am not sure why they choose this (whether it was a data driven decision or a guess). However, I have re-evaluated some of our Tale 5 data that seemed inconsistent. Specifically, some tests that yielded non-overlapping ranges, given our range method calculations. Using the ceiling function approach gave consistent data! This may be a mistake, but I am updating all my calculations to the Ceiling Function Rounding method of previous tales.

Ceiling Floor Function: This means that the rounding function will always round UP to the next whole number.

given a gene target x out of gene length y, the range is:

• minimum range is (x-1)/y x 100%
• maximum range is x/y x 100%

For example, a mutagen that targets gene 8 out of a possible 27 has minimum range 7/27 x 100% = 25.926% and maximum range of 8/27x100% = 29.629%.

given a mutagen range, the targeted gene will always be:

• minimum gene is [(minimum range)x(genome length)]/100 rounded up
• maximum gene is [(maximum range)x(genome length)]/100, rounded up

For example, a mutagen that has range 25.926% to 29.629% targets, on a genome of length 27:

minimum gene is (25.926)x(27)/100 = 7.000002 rounded up to 8 maximum gene is (29.629)x(27)/100 = 7.99983 rounded up to 8.

Please see http://www.atitd.org/wiki/tale4/Mutagens#Advanced_Mutagen_info for more examples.

Tayete Recipes

Tay 01

Dry, Prickly, Reticulated Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 41/Ava 42) Observed:

Left Spoint: 1 weed/1 water/1 seed/2 flax/1 rotten flax

Right Splint: 0 zeed!!

Right splint had no gene groups broken, but gained an additional gene sequence of RRGY Left splint had the (RO) gene group broken.

Solvents on Left Splint showed:

• ORR
• GYG
• YRR <-- that pinpoints it as the third G directly after (GYGYGY) as being replaced with an R

Right Genome: K(OR)GR(GYGYGY)RRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 11/27.

gene range is 10/27 to 11/27 or 37.037% to 40.740%

also reveals that a "G" gene was moved to the left splint

Left Splint:

The phenotype revealed that the (RO) seed gene was mutated.

The right gene results show that an "R" gene was switched w/ a "G" gene.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(GO)YY(OR)RK

gene targeted was 21/27

gene range is 20/27 to 21/27 or 74.074% to 77.777%

TEST 2: Fracture / Crown (Ava 43,44)

phenotypes: Fracture became giant, colors unchanged. Crown petals went from red to magenta

Left splint: Fracture: K(ROYG)(ROY(G)YOR)GOR(GOOO)K

Test 1 predicts gene 7 or 8 will be swapped

if 7, 1st Y in the "ROYGYOR" set is swapped, it should be a 1 giant + 1 dwarf = small size.

if 8, then G in middle of "ROYGYOR" set is swapped, both sets are broken, and result is 1 giant.

observed: its a giant. no color changes

it was gene 8 swapped.

range predicted now is 38.888% to 44.444%

this updates the left splint range to: 38.888% to 40.700%

Right splint: Crown: K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Test 1 predicts that gene 28 or 29 will swap. (O or 2nd R in the first (UROR) set. If so, a yellow gene will be broken.

observed: the petals are more magenta colored than red. This is consistent w/ above prediction. It could also be a gene before or after this one that moved, and give the same results, so the exact gene position isn't certain w/o solvent testing.

TEST 3: Dusk / Fracture (Ava 72, 73)

phenotypes: no changes were observed !!

Expected Left: gene 29 or 30. If 30 (GRRO) --> (GRRR)removing Yellow north and adding cyan south If 29 (GRRO) --> GRRO no change at all.

Expected Right--> 14th gene, R from GOR to swap in. GOR is junk, so no phenotype change is expected.

Results: no differences in phenotypes.

therefore Left splint targeted gene 29. R-->R.

genomes are the same.

range from this is: 28/73 to 29/73 or 38.356% to 39.726%

updated range is: 38.888% to 39.726%

TEST 4: Vampire / Vampire (Bessodia's Test)

Vampire genome is: KIYIYIOIYIOIOK 12 genes long Left splint:

• IYIOI
• YOOIY
• YIOIO
• YIYIO
• OOIYI
• IYOOY
• OIOK
• KIYIY
• IYIYO
• YIYOO

an I gene swapped for an O gene: KIYIYOOIYIOIOK 5th gene was targeted. range is 4/12 to 5/12 or 33.333% to 41.666% that's consistent w/ the other test results!

Right splint:

• IOIY
• IYIO
• YIII
• IYII
• IIIO
• IYIY
• IIOK
• KIYI
• IIIO
• OIYI

genome is: KIYIYIOIYIIIOK 10th gene was targeted. range is 9/12 to 10/12 or 75.000% to 83.333%. that narrows the range down, when combined w/ other tests so the range is now 75.000% to 77.777%

TEST 5: Crown/blush (Ava 174,175)

Expect: Crown to hit gene 15 or 16 (R,V) and Blush to hit gene 21 (O) Right splint:

Observe: Blush lost 1 magenta on middle and top of stamen, and gained 1 cyan on top stamen.

VIOO --> VIRO gene 20 was picked.

range is 19/26 - 20/26 or 73.076% to 76.923%

new range for right splint is 75.000% to 76.923%

Left Splint:

observed no change, so gene 15 was picked.

gene range is 14/37 to 15/37 or 37.837% to 40.540%.

this is consistent w/ other tests.

Tay 02

Calico, Green, Striped Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 37/Ava 38)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• RGRO
• GRGY
• ORYY
• ROYY
• GROY
• GRG
• GYOR
• YORY
• YGYG

no anomolies found yet. Standard Junk genes all covered

Solvents on Left Splint showed:

• GRGY
• ORYY
• YGRG
• GROY
• RGRO
• ORGR
• GYGY
• YGYG
• GYOR
• YRG
• ORRK

no anomolies found, and standard junk genes all covered.

this test is inconclusive

TEST 2: Silken / Vampire (Ava 26, 27)

phenotypes: Silken bulb unchanged, Vampire had center go magenta (ie had an IO get broken)

Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK

Right splint: Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K

Left Splint:

• IIIK
• RRRO
• UIII
• YYYG
• YYYY <-- pinpoints as the last O --> G

genome is KRRROOYYYYGGGUUUIIIK 6th out of 18 gene targeted range is 5/18 to 6/18 which is 27.777% to 33.333%

Right Splint: know from phenotype we lost an IY, know from above we had Y-->O. so we trade IY for IO.

• YIOI
• KIYI
• IOIO
• IOIYI <-- pinpoints as the second Y

genome is KIYIOIOIYIOIOK 4th gene out of 12 range is 3/12 to 4/12 which is 25.000% to 33.333%

TEST 3: Vampire/ Vampire (Bessodia's Test) Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K

no visual different between the flowers. solvent testing showed no changes. presuming the same gene type swapped. No conclusions available.

TEST 4: crown/vampire (ava 188, Ava 189)

Left:

expected to hit gene 11 -13 (OOV)

observed: Mag W was broken. So it was gene 11 or 12 (the O's in UROO

range is 10/37 to 12/37 = 27.027% - 32.432%

Used Solvent: found OYVU, shows, that gene 12 was targeted

range is 11/37 to 12/37, or 29.729 - 32.432%

new left range is 29.729% to 32.432%

Right:

expected to hit gene 4 or 5 (Y OR I)

observed: IY broke and got IO, so it was gene 4 that ws hit

range is 3/12 to 4/12 or 25.000% to 33.333%.

This is consistent w/ previous tests.

Tay 03

Calico, Mottled, slimy Renard opened

TEST 1: Nile Green / Nile Green (Ava 35, Ava 36) Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

Left Splint: 1 weed/1 water/1 seed/2 flax/0 rotten

Right Splint: 1 weed/01 water/1 seed / 2 flax/ 0 rotten

Both splints had junk genes swapped.

Solvents on Right Splint showed:

• GGYG <-- that pinpoints it as 12th gene, R being replaced with G

Right Genome: K(OR)GR(GYGYG)GG)GY(OR)YYRG(OR)YY(OR)RK

gene targeted was 12/27.

gene range is 11/27 to 12/27 or 40.749% to 44.444%

also reveals that a "R" gene was moved to the left splint

Left Splint:

Looking for where a junk G was replaced with a R gene, and no new genes were created. (Phenotype was not altered)

Solvents on Left Splint showed:

• ORGR
• GYGY
• YGYG
• YORY
• RYYR
• ROYY
• RGR
• GYOR
• RGYO
• YYO
• YGY
• RGYG
• YRR <--- confirms G --> R switch. Only two places it could be. 11th or 20th.
• RRRO <-- proves 20th gene was swapped to R.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRR(RO)YY(OR)RK

gene targeted was 20/27

gene range is 19/27 to 20/27 or 70.370% to 74.074%

TEST 2: Silken / Clarity (Ava 28, Ava 29)

phenotypes: Silken bulb unchanged, Clarity had a cyan gene broken.

Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK

left target 13 or 14 /18 gene --> 1st or 2nd U.

Right splint: Clarity Genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GORR)GRORGRROK

right target is 15 or 16/36 gene --> 1sr or 2nd R in the first cyan gene set (GORR)

Solvent:

• IRIR
• RIOI
• OIYI
• UGRO <-- pinpoints as the 2nd R in (GORR), and the 16th gene

16 gene out of 36 range is 15/36 to 16/36 or 41.667% to 44.444%

resultant right genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GORU)GRORGRROK

Left Splint Solvent:

• GGGR. That confirms it.

we know now what left genome is, since it's U was replaced with an R from Clarity:

resultant left genome: KRRROOOYYYGGGRUUIIIK

13th gene targeted.

range is 12/18 - 13/18 = 66.667% to 72.222%

Combining with Test 1 results, new Left range is 70.370% to 72.222%

TEST 3: Vampire/ Vampire (Bessodia's Test) Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K

Left Splint:

• YOOI
• IOIY
• IYIO
• IYOO
• YIYI
• IYIY
• OOIO
• OIYO
• YIOI
• IOIK
• KIYI

9th gene was swapped: : K(IY)(IY)(IO)(IY)OO(IO)K range is 8/12 to 9/12 = 66.667% to 75.000%. That is consistent w/ other tests.

Right Splint:

• IYII
• IIIY
• IOIK
• IIYI
• KIYI
• YIOI

6th gene was swapped: KIYIYIIIYIOIOK range is 41.667% to 50.000%. This is consist with other Test findings.

TEST 4: Crown / Fracture MEANT to do crown / clarity....oops!

yielded Ava 121, Ava 122 Left: Broke UROR (Yellow West). Not sure if gene 27 (R) or gene 28(O) w/o using solvents.

Right: (ROYG)(ROY_YOR)GOR(GOO) broke that middle _ gene (was a G). it broke up a giant and a dwarf gene, leaving just 1 giant gene in the genome. can't tell what was swapped in, w/o solvent.

test inconclusive, w/o solvents.

TEST 5: Crown/Clarity (Ava 129. Ava 130)

expected: Crown's gene 27 or 28 ... UROR... (the RO in the middle) expected: Clarity's genes 15, 16, 17. gene 15, 16 are R's in GORR gene 17 is G in GROR

Observed: phenotypes of flowers are the same!! so both splints swapped R's.

Left: gene 27 of 37 was targeted range is 26/37 to 27/37 or 70.270% o 72.975%. this is consistent w/ other tests, but a larger range.

Right Splint: was gene 15 or 16 of 36 total range is 14/36 to 16/36 or 38.888% to 44.444% this is consistent w/ other tests, but a larger range.

Tay 04

Cracky, Prickly, Spongy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 49/Ava 50)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• not tested yet

Solvents on Left Splint showed:

• KORG
• ORGR
• RYY
• YGY, GYG
• YORY
• GROY
• ORYY
• GYGY
• RGRO
• YYRG
• YRGR
• GYGR
• RGYO

no anomolies found, and standard junk genes all covered. Seems that the Same Gene type was swapped between splints :( further tests are needed to characterize this mutagen

TEST 2: Vampire / vampire (Bessodia's Test) Vampire: KIYIYIOIYIOIOK 12 genes

Right splint:

• IYIO
• YYIO
• YIOI
• KIYY
• IYYY
• IOIY
• OIYI
• IYYY
• 0IOK

3rd gene was swapped: KIYYYIOIYIOIOK range is 2/12 to 3/12 which is 16.667% to 25.000%

Left Splint:

• KIII5

2nd gebe was swapped. KIIIYIOIYIOIOK range is 1/12 to 2/12 which is 8.333% to 16.667%

TEST 3: Blush/morning (Ava 170, Ava 171)

expect gene 3,4, 5 on blush (ORV)

expect gene 9,10,11,12,13 on morning (IYYYU)

Observe: Left lost Mn, so it was Gene 3 or 4 targeted Right lost 1 yellow middle and lower stamen, so it was gene 11 targeted.

Left had gene 3 of 26 picked

range is 2/26 to 4/26 or 7.692% to 15.384%

new range is 8.333% to 15.384%

solvents will further narrow this

RIght had gene 11 of 44 picked

range is now 10/44 to 11/44 or 22.727% to 25.000%

TEST 4: Clarity/Crown (Ava 202, Ava 203)

expect gene 9 or 10 on Crown to be hit

observed that crown lost Mw only, so a UROO was broken up somehow.

expect clarity's gene 3,4 or 5

observed that clarity lost an IR (gene 3 or 4)

it had to be gene 4 (an I swapped would have given crown stamen colors!

Left Splint: gene 4 of 36 was targeted.

gene range is 3/36 to 4/36 or 8.333% to 11.111%, which is a new range.

Right Splint: gained an R.

UROO was broken, so --> RROO

gene 9 was targeted, of 37

gene range is 8/37 to 9/37 or 21.621% to 24.342%

new gene range is 22.727% to 24.324%

TEST 5: fracture/vampire (Ava 242, Ava 243)

Expect: vamp to donate gene 3, I and Fracture to swap either 2 or 3 (OY in ROYG)

observed: vamp lost IY as expected

fracture gained an IY color and lost giant.

Therefore hit gene 2 on left splint (ROYG --> RIYG)

left range is 1/18 to 2/18 or 5.555% to 11.111%

this is consistent w/ previous results.

Tay 05

Crackly, Dry, spongy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 39/Ava 40)

Both splints had junk genes swapped, or same type of gene swapped. Solvents on Right Splint showed:

• not tested yet

Solvents on Left Splint showed:

• GYGR
• RYYR
• YORR
• GROY
• OYY
• YRG
• RGR
• GYOR
• RGYO
• GYG
• OYYO
• ROYY
• GRGY

no anomolies found, and standard junk genes all covered. Seems that the Same Gene type was swapped between splints :(

TEST 2: Blush / Morning (Ava 31, 32)

phenotypes: Both phenotypes were unchanged

Left splint: Blush Genome: KGRORVGOOOVUROOVIOVIOOVIOOOK

Right splint: Morning: KIRRRIYYYIYYYUYYYUYYYURORURORGRRRGYYYGORRGRROK

morning has no junk genes, therefore the same type of gene must have been swapped.

so far, no conclusions made

TEST 3: Vampire / vampire (Bessodia's Test) Vampire: KIYIYIOIYIOIOK 12 genes

Right splint:

• IIOI
• IIIO
• IYIY
• IYIO
• IOIO
• YIYI
• IOII
• KIYI
• OIOK

8th gene was swapped: KIYIYIOIIIOIOK range is 7/12 to 8/12 which is 58.333% to 66.667%

Left Splint:

• YIYI
• IOYY
• YIOI
• OIOK
• IOYY
• KIYI
• OYYI

7th gene was swapped. KIYIYIOYYIOIOK range is 6/12 to 7/12 which is 50.000% to 58.333%

div style="margin:30px"> TEST 4: Fracture/Vampire (Ava 176, Ava 177)

Observed:

Left: broken dwarf, but kept 2 giant gene sets. So it targetd gene 9, 10, 11 (YOR)

Right: got an IO gene and lost an IY gene --> lost an O, and gained aY

Left splint had gene 10 targeted (the Y) of 18

left range is 50.000% to 55.555%

this is a new range!

right splint had gene 8 picked, of 12

gene range is 7/12 to 8/12, or 58.333 to 66.666%

this is the same range as above.

Tay 06

Fuzzy, Hairy, SPongy, Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 33/Ava 34) Observed:

Left Spoint: 1 weed/1 water/1 seed/2 flax/0 rotten

Right Splint: 2 weed/0 water/1 seed / 1 flax/ 0 rotten

Right splint was affected in (GYGYGY) gene, specifically NOT the middle GY genes. Left splint had a junk gene swapped.

Solvents on Right Splint showed:

• RYY
• RGYG
• GGRG <-- that pinpoints it as the third Y in (GYGYGY) being replaced with a G

Right Genome: K(OR)GR(GYGYGG)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 10/27.

gene range is 9/27 to 10/27 or 33.333% to 37.037%

also reveals that a "Y" gene was moved to the left splint

Solvents on Left Splint showed:

• KOR
• ORRK
• RGY
• YYOR
• ROOY
• ORYY
• GYY <--- proves it

Looking for where a junk G was replaced with a Y gene, and no new genes were created. (Phenotype was not altered)

Left Genome: K(OR)GR(GYGYGY)YRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 11/27

gene range is 10/27 to 11/27 or 37.037% to 40.740%

TEST 2: Ava 45 / Blush

This is Step #2 in section below, trying for 75% green flower. Left: (UYYY) yellow gene was broken on Ava 45. need to do solvent testing to know which gene it was exactly.

Right: predicted either gene 9 or 10 to swap.

gene 9 would have broken (GOOO)

Gene 0 is a V. no phenotype change expected.

No phenotype change of blush was seen. Therefore, we know 10th gene V swapped. 10/26 gene targeted range is 9/26 to 10/26 = 34.615% to 38.461% combining with test 1, that gives overlap range of 34.615% to 37.037%.

TEST 2: Vamp/Crown (Ava 182, Ava 183)

Expect Vamp to donate gene 5, I... and Crown to hit 13 or 14, V or U

Observed: Crown gained an IR gene (and vamp lost an IO).

therefore, crown was hit at gene 14, U--I.

Right splint: gene 14 of 37 was targeted

range is 13/37 to 14/37 or 35.135% to 37.837%

new range for right splint is 35.135% to 37.037%

Left splint: gene 5 was targeted.

range is 4/12 to 5/12, or 33.333% to 41.666%

This is consistent with above tests.

Tay 07

Crackly, Hairy, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 51/Ava 52) Observed:

Left Spoint: 2 weed/1 water/1 seed/2 flax/0 rotten had an extra weed!

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten not changed

Left splint was affected in (OR) gene. Right splint had a junk gene swapped.

Solvents on Left Splint (Ava51) showed:

• YYOY <-- that pinpoints it as the last (OR) broken. R was replaced w/ Y.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OY))RK

gene targeted was 26/27

gene range is 25/27 to 26/27 or 92.592% to 96.296%

Right splint had a junk Y replaced. Solvents on Right Splint showed:

• GRGY
• GROY
• GYGY
• RGRO
• RGRG
• RRK
• YRG
• YYO
• YOR
• ORG
• ORR <--- pinpoints it (after other solvents eliminated some possibly Y's).

The 17th Y gene is swapped out and was replaced with an R

Right genome: K(OR)GR(GYGYGY)GRGY(OR)RYRG(RO)YY(OR))RK

gene targeted was 17/27

gene range is 16/27 to 17/27 or 59.259% to 62.963%

TEST 2: Clarity / Morning (Ava 69, Ava 70)

Left: this will swap either the 34th or 35th gene in Clarity. Both are R genes.

.... GRROK.

Right: this will swap either the 27, 28 or 29 gene (of 44) in Morning.

original genome: ...(UROR)(GRRR)...

if 27th, (UROR) --> (URRR). This will remove Yellow Inner West and add Cyan Inner East. if 28th (UROR) ---> (UROR). No change in genome or phenotype if 29th (GRRR)--> RRRR. this removes cyan from the outer south, and adds nothing.

Observed: Yellow inner west was removed and cyan inner east was added. Right genome:KIRRRIYYYIYYYUYYYUYYY(UROR)(UR(OR)R)GRRRGYYYGORRGRROK

gene targeted was 27/44.

gene range is: 26/44 to 27/44 or 60.227% to 62.500%.

combined with TEST 1's results, Right splint range is now: 59.259% to 61.363%

Left: GRRO gene was indeed broken, removing a yellow and leaving, in total, 1 black and 1 blue color on the Outer North Petals. No further conclusions can be made about which R gene was swapped.

TEST 3: Vampire/vampire (Bessodia's Test) Left genome: IYIYIOIOIOIO. 8th gene 0-->Y range is 58.333% to 66.666% this is wider than already known range.

Right genome: IYIYIOIYIOIY 12th gene Y-->O range is 91.666% - 100% this is wider than already known range.

TEST 4: Crown / Fracture (Ava 125, Ava 126)

expect: gene 35 or 36 in crown (center RO in UROR gene). Fracture just donates an R.

Observed: Left has 1 cyan east and lost 1 yellow west.

so gene 36 was swapped .. UROR --> URRR

gene 36/37 targeted

genome: UROO UROO UROO V URVOOVURVORV UROR UROR URRR

range is 35/37 to 36/37 or 94.594% to 97.297%

combining with other test results, new range is 94.594% to 96.296%

Right: colors are the same, but its a giant x 2!!

we know it was an R being swapped out with an O.

genome: ROYG ROYGYOO GOR GOOO

gene 11/18 was targeted

range is 10/18 to 11/18 or 55.555% to 61.111%

new range is 59.259% to 61.111%

Tay 08

Crackly, Phosphorescent, Slimy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 53/Ava 54) Observed:

Left Spoint: 0 weed/1 water/1 seed/0 flax/0 rotten ... no weed and no flax are different

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step

Left splint was affected in ROY sequence (which is required to harvest flax, see flax genome theories for more info). However, it was not a *(RO) that was changed, or the seed count would have gone down. Furthermore, we need a new OR gene, to account for the 1 less weed step.

looks like the 2nd to last Y should be switched to a R. ... YYRG(RO)RY(OR)RK. This would add (OR) and removed the (ROY).

Solvents on Left Splint showed:

• GYG
• RGR
• ROR <-- that pinpoints it as the gene we suspected.

the 23rd gene, Y, is replaced with an R

gene range is 22/27 to 23/27 or 81.481% to 85.185%

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)RY(OR)RK

Right splint lost an (-1 weed) gene, so (OR) was broken somewhere in the genome. We know a R was replaced with a Y, from the left splint tests.

Solvents on Right Splint showed:

• ORY
• ROY
• GRGY
• GYGY
• YOR
• ROYY
• YYRG
• RGR
• OYYO
• YORY
• KOY <--- shows its the second gene, R, that is swapped with a Y from the other splint

gene targeted was 2/27.

gene range is 1/27 to 2/27 or 3.703% to 7.407%

Right Genome: K(OY)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

TEST 2: Crown / Ava 28 (Ava 123, Ava 124)

Expected: Crown will have gene 31 or 32 targeted (center RO in UROR gene set) If 31, there will be no color change (R --> R) If 32, we will lose Ywest and gain Ceast (UROR --> URRR)

Ava 28 is just donating an R from position 1 or 2.

Observed: Left: Cyan East is present, and one Yellow west was lost. so Gene 32 was targeted. Genome: UROOUROOUROOVURVOOVURVORVUROR URRR UROR range is 31/37 to 32/37 or 83.783% to 86.486% combining with other test results, the range is now 83.783% to 85.185%

Right: original genome of Ava 28: KRRROOYYYYGGGUUUIIIK either gene 1 or 2 was swapped, so resulting genome is: KORROOYYYYGGGUUUIIIK OR KROROOYYYYGGGUUUIIIK.

Solvent showed:

• RORO

so its hitting gene 2/18

range is 1/18 to 2/18 or 5.555 to 11.111%

after test 3 results, range is now 5.555% to 7.407%

TEST 3: vampire/crown (Ava 180, Ava 181)

Expect: vampire to donate gene 10, O

crown will hit gene 2 or 3, RO in URRO

if gene 2, vamp loses IO, gains IR/crown loses Mw, gains Ms

if gene 3, O changes with O, no changes

Observed:

left, vampire gained magenta on middle and lower stamen, lost mage on upper stamen

right, crown gained IO, lost Mw

Left was gene 11! IYIYIOIYIOOO now.

gene was 11 / 12

gene range is 10/12 to 11/12 or 83.333% to 91.667%

this is consistent w/ range.

RIght was gene 3 of 37

gene range is 2/37 to 3/37, or 5.405% to 8.108%

right range is now 5.405% to 7.407%

Tay 09

Calico, Green, Smelly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 55/Ava 56)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• GRG
• GRO
• GYGY
• YGYG
• ORYY
• ORGR
• YORY
• YYOR
• RGYO
• OYYO
• GYGR
• RYY
• YRGR
• ORRK

no anomolies found yet. Standard Junk genes all covered.

This mutation swapped same gene type between splints

this test is inconclusive

TEST 2: Vampire/Vampire (Bessodia's Test)

R: KIYOYIOIYIOIOK 3rd gene was switched range is 2/12 to 3/12 or 16.666% to 25.000%

Left: KIYIYIIIYIOIOK 6th gene was switched range is 5/12 to 6/12 or 41.667% to 50.000%

TEST 3: Silken/Blush Ava 24/Ava25

observed: blush lost magenta south, silken still white

Right Splint (Ava 25) original genome is blush:GRORVGOOOVUROOVIOVIOOVIOOO expected target range is gene 5 to 7 Solvent:

• VURO
• VYOO
• OOVU
• OVIO
• OOVI
• ORVY

6th gene was target G --> Y correct genome is:GRORV Y OOOVUROOVIOVIOOVIOOO range is 5/26 to 6/26 or 19.230% to 23.077, a new range

Left Splint (Ava 25) original genome is silken: RRROOOYYYGGGUUUIII we know it gave a Y to other splint and got a G Solvent:

• UUII
• UIII
• OOYY
• KRRR
• GGGG <-- pinpoints as the last Y being swapped for a G

gene is 9th of 18 range is 8/18 to 9/18 or 44.444% to 50.000%, a new range. correct genome is: RRROOOYYGGGGUUUIII

Range on right splint narrowed down to 20.454% to 22.727% See Test 2 under section for getting 2 shades of cyan on inner stamen

TEST 5: Clarity/Crown (Ava 200, Ava 201)

expect clarity to hit gene 17-19 (GRO in GROR) and crown to hit gene 8 or 9 (last O in UROO or U in UROO)

observed clarity broke Mn and Crown broke Mw and gained Yw.

this means left hit 18 (GROR -->GOOR) and right hit 8 (UROO --> UROR)

Left splint targeted gene 18 of 36

range is 17/36 to 18/36 or a new range of 47.222% to 50.000%

Right splint targeted gene 8 of 37

range is 7/37 to 8/37 or 18.91% to 21.621%

new range of right is 20.454% to 21.621%

Tay 10

Crackly, Spotted, Striped , Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 57/Ava 58) Observed:

Left Spoint: 2 weed/0 water/1 seed/0 flax/0 rotten

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten

Left splint was affected in (GYGYGY) gene, specifically in the middle GY genes. This breaks up both Flax genes (GYGY) as well as the water gene (GYGYGY), which would account for our observations.

Solvents on Left Splint showed:

• YGRG <-- that pinpoints it as the middle Y in (GYGYGY) being replaced with a R

Left Genome: K(OR)GR(GYGRGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 8/27.

gene range is 7/26 to 8/27 or 25.925% to 29.629%

Right splint had a -1 weed gene broken (OR). From above, we know it was the R that was swapped with a Y from the Left splint. Just have to identify which of the three (OR) genes was broken. Solvents on Right Splint showed:

• RGRG
• RGY
• GYG
• YRGY
• RGY
• ORG
• YGY
• ORY
• RGY
• YOR
• YYR
• RRY
• GYO
• OYRB <--- pinpoints the (OR) gene at the end as switching to (OY).

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OY)RK

gene targeted was 26/27

gene range is 25/27 to 26/27 or 92.592% to 96.296%

TEST 2: morning / Ava 28 yields flowers Ava 103, Ava 104

Left: Above test 1 predicts gene 12-14 will be chosen, and that Ava 28 will donate a "I" gene morning genome: K IRRR IYYY IYYY UYYY ... if 12th gives ... IYYI UYYY... would lose 1 yellow on inner stamen if 13th, gives ... IYYY IYYY... would lose 1 yellow East, gain 1 yellow on entire stamen if 14th, gives ... IYYY UIYY... would lose 1 yellow East, gain 1 yellow on outer and middle stamen

Observed: top and middle staymen are same. Inner stamen is more blueish. East petals are the same. therefore, the 12th gene was exchanged. so the range is 11/44 to 12/44 or 25.000% to 27.272% combining that with Test 1 results, the new range is 25.925% to 27.272%

Right: Test 1 predicted that gene 17 or 18 would be switched. Ava 28 genome is: KRRROOOYYYGGGRUUIIIK Either is an I gene. if 17th, gives ...UUIYIK would introduce yellow on the top stamen if 18th, gives ...UUIIYK would still appear all white.

Observed: the top stamen has 1 shade of yellow now. therefore, the 17th gene was swapped. the range is 16/18 to 17/18 or 88.888% to 94.444% combining hat with Test 1 results, the new range is 92.592% to 94.444%

TEST 3: Vampire/Vampire (Bessodia's Test)

Left: KIYIOIOYIOIOK 4th gene swapped range is 3/12 to 4/12 or 25.000% to 33.333% consistent w/ above tests.

Right: KIYIYIOIYIOIK 12th gene is switched range is 91.667% to 100% consistent w/ above tests.

TEST 4: crown/Ava 24 (for silken) (Ava 186, Ava 187)

Left:

Expected: gene 10 or 11 to be hit, R and O in UROO.

observed: Mw was broken, but gained an IOO.

Gene 10 must have been hit, UROO --> UIOO.

range is 9/37 to 10/37 or 24.324% to 27.027%

new range for left splint is 25.925% to 27.027%

Right:

Expected gene 17 to swap, the middle I.

observed: gained an IR. consistent w/ above. I-->R on middle I gene.

Gene 17 of 18 was hit.

range is 16/18 to 17/18 or 88.888% to 94.444%

new range for right splint is 92.592% to 94.444%

Tay 11

Phosphoresecent, Slimy, Smelly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 59/Ava 60) Observed:

Left Spoint: same phenotype

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step

Right splint had an extra weeding step. This means an (OR) gene was broken up.

Solvents on Right Splint showed:

• YRRR <-- that pinpoints it as the last O gene --> R gene.

the 25nd gene, O, is replaced with an R

gene range is 24/27 to 25/27 or 88.888% to 92.592%

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(RR)RK

Left splint lost an R gene in the junk genes somewhere, which was replaced with an O gene. Solvents on Left Splint showed:

• YGRG
• OGRO <--- shows its the 19th gene, R, that is swapped with a G from the other splint.

gene targeted was 19/27.

gene range is 18/27 to 19/27 or 66.666% to 70.370%

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYOG(RO)YY(OR)RK

TEST 2: Vampire/vampire (bessodia's test)

observed: no change in phenotype, same gene was swapped

expected gene 9 or 10 on left (Y,I) and gene 11 or 12 on right (I,O)

must have been I--> I

left genome is gene 9/12

range is 8/12 to 8/12 or 66.666% to 75.000%

that is consistent

Right genome is gene 11/12

range is 10/12 to 11/12, or 83.333% to 91.666%

new range on right is 88.888% to 91.666%

Tay 12

Calico, Crackly, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 61/Ava 62)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• ORYY
• GYGR
• ROYY
• ORGR
• YRGR
• YYOR
• RGYG
• RGRG
• GYGY
• GRGY
• GYO
• RGRO
• RRK

no anomolies found yet. Standard Junk genes all covered.

This mutation swapped same gene type between these splints.

This test is inconclusive

TEST 2: Vampire/Vampire (Bessodia's Test)

Left: KIOIYIOIYIOIOK 2nd gene switched range is 1/12 to 2/12 or 8.333% to 16.667%

Right: KIYIYIYIYIOIOK 6th gene switched range is 5/12 to 6/12 or 41.667% to 50.000%

TEST 3: Ava 25/Ava 25

Ava 25 flower is blush with 1 mutated gene: GROR V YOOO V UROO V IO V IOO V IOOO Expected range: left gene 3-5 (O,R,V) , right gene 12-14 (R,O,O)

Ava 133 / Ava 134 result

Observed: Left: Magenta west, all other petals are white. therefore, GROR was broken Right: Magenta north, all other petals are white. Therefore, UROO was broken

Left Analysis: was not gene 5 (V) or there would have been no phenotype change. was not gene 3(O) or would be either GROR or GRRR, both are colors that are not present. it was gene 4 (R) of 26 total. range is 3/26 to 4/26 or 11.538% to 15.385%, which is a new narrowed range.

Right Analysis: got an R, lost an O. (otherwise, there would be no pheno change with R-->R) was gene 13 or 14. range is 12/26 to 14/26 or 46.153% to 53.846% Combining with old range, new range is 44.153% to 50.000%.

• could narrow this further w/ solvent testing.

New range for left splint: 13.636% to 15.384% See Test 3 under section for getting 2 cyan Inner stamen for details

Tay 13

Calico, Slimy, Smelly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 63/Ava 64) Observed:

Left Spoint: no seeds!

Right Splint: no change in phenotype

Left splint was affected in (RO) gene, since it gave 0 seeds. Left splint had a junk gene swapped, to either a R or an O.

Solvents on Right Splint showed:

• YORR
• YGYG
• ORYY
• ROYY
• YORY
• YYOG <-- that pinpoints it as 3rd Junk R gene, being replaced with an O.

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYOG(RO)YY(OR)RK

gene targeted was 19/27.

gene range is 18/27 to 19/27 or 66.666% to 70.370%

The above pinpoints that the O gene in the (RO) gene set was replaced with an R.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRGRRYY(OR)RK

gene targeted was 22/27

gene range is 21/27 to 22/27 or 77.777% to 81.481%

TEST 2: Vampire/Vampire (Bessodia's Test)

Left: KIYIYIOIYIIIOK 10th gene switched range is 9/12 to 10/12 or 75.000% to 83.333%

Right: KIYIYIOIYOOIOK 9th gene switched range is 8/12 to 9/12 or 66.666% to 75.000%

both are consistent w/ above test.

Tay 14

Fuzzy, Reticulated, Spotted, Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 65/Ava 66) Observed:

Left Spoint: 1 extra weed

Right Splint: 1 extra seed

This means a new (RO) gene was created in the right gene, while a (OR) gene was broken in the left gene.

Solvents on Right Splint (Ava 65) showed:

• ROR (narrows it down to one of the end Y genes. Specifically, RGR could not become ROR, or there would be a new (OR) gene made as well, and it would be 0 weeds.)
• YGR
• YYR
• YOR
• KOR
• GRO
• YROR <-- that pinpoints it as the last Y in the genome being replaced with a R.

gene targeted was 24/27.

gene range is 23/27 to 24/27 or 85.185% to 88.888%

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YR(OR)RK

Left Splint will have an R in (OR) gene replaced with a Y gene. Solvents on left Splint showed:

• RGY
• YORY
• YYOY <--- proves it to be the 2nd last R in genome.

gene targeted is 26/27.

gene range is 25/27 to 26/27 or 92.592% to 96.296%

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YYOYRK

TEST 2: Vampire/Vampire (Bessodia's Test)

Left: KIYIYIOIYIOIIK 12th gene switched range is 11/12 to 12/12 or 91.666% to 100%

this is consistent w/ above range

R: KIYIYIOIYIOOOK 11th gene is switched range is 10/12 to 11/12 or 83.333% to 91.666%

this is consistent w/ above range

TEST 3: Vamp / Crown (ava 230, Ava 231)

Observed: vamp lots IO gained IR.

This is consistent with gene 12, also result from Test 2.

Observed:CRown had UROR broken

above predicted gene 32 or 33. but we know it had to be the R gene swapped to other splint.

therefore, gene 33/37 was targeted

range is 86.486% to 89.189%

new range is 86.486% to 88.888%

Tay 15

Fuzzy Green Mottled Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 86/Ava 87) Observed:

Left Spoint: no water and no flax!

Right Splint: 1 water, 2 weeds. (one extra weed step)

Left splint had (GYGYGY) gene groups broken in the middle GY Left splint had an(OR) gene group broken.

Solvents on Left Splint showed:

• ORGR
• KORG
• GYRY <-- that pinpoints it as (GYGYGY) --> (GYRYGY)

Left Genome: K(OR)GR(GYRYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 7/27.

gene range is 6/27 to 7/27 or 22.222% to 25.925%

also reveals that a "G" gene was moved to the right splint

Right Splint:

The phenotype revealed that the (OR) -1 weed gene was mutated.

The right gene results show that an "R" gene was switched w/ a "G" gene. So we are looking for (OR) -->OG

Solvents on Right showed:

• YYRG
• GROY
• RGRO
• GYOR
• OGGR <-- pinpoints as the first (OR) changed.

Right Genome: KOGGR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 2/27

gene range is 1/27 to 2/27 or 3.703% to 7.407%

TEST 2: Vampire/Vampire (Bessodia's Test)

Left: KIYIIIOIYIOIOK

4th gene switched

range is 3/12 to 4/12 or 25.000% to 33/333%

narrows the range to 25.000% to 25.925%

Right: KYYIYIOIYIOIOK

1st gene switched

range is 0/12 to 1/12 or 0% to 8.333%

consistent w/ above range, but doesn't narrow it.

Tay 16

Calico, Fuzzy, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 148/Ava 149) Observed:

Left Spoint: no water, 2 weed, no flax

Right Splint: normal

Left splint had the center G or Y in (GYGYGY) broken

Solvents on Left Splint showed:

• RGYR <-- that pinpoints it as the middle G in(GYGYGY) as being replaced with an R

Left Genome: K(OR)GR(GYRYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 7/27.

gene range is 6/27 to 7/27 or 22.222% to 25.925%

also reveals that a "G" gene was moved to the left splint.

Right Splint had an R in junk genes targeted solvents on Right splint showed:

• GROY
• YYOR
• YYGG <-- pinpoints 19th gene R --> G

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYGG(RO)YY(OR)RK

gene targeted was 19/27

gene range is 18/27 to 19/27 or 66.666% to 70.370%

TEST 2 Crown/Energy (Ava 198, Ava 199)

Expect: Crown to target gene 9 or 10 (U,R in UROO) and energy to target gene 29 or 30 (O of GRRO and U of UROR) Observed: Crown lost Mw and gained Me so UROO --> UOOO

Energy lost Yn and gained Cs (GRRO --> GRRR)

Left splint: gene 10 of 37 was targeted

range is 9/37 to 10/37 or 24.324% to 27.027%

new left range is 24.324% to 25.925%

Right splint: gene 29 of 42 was targeted.

range is 28/42 to 29/42 or 66.666% to 69.047%

new right range is 66.666% to 769.047%

Tay 17

Calico, Ret, Slimy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 150/Ava 151) Observed:

Left Splint: 2 weed, 1 water

Right Splint: normal

Left splint had one of the three (OR) genes broken

Solvents on Left Splint showed:

• YORR
• RYYR
• GYGR
• YYRG
• KORG
• RGYG

the only (OR) gene not coveredis the O in the second genes set

gene targeted was 15/27

gene range is 14/27 to 15/27 or 51.851% to 55.555%

also reveals that a O was moved to the Right splint.

Right Splint had junk gene targeted solvents on Right splint showed:

• YGYG
• OYYO
• ROYY
• RYYR
• GROY
• GOOR <-- pinpoints 14th gene Y --> O

Right Genome: K(OR)GR(GYGYGY)GRGO(OR)YYRG(RO)YY(OR)RK

gene targeted was 14/27

gene range is 13/27 to 14/27 or 48.148% to 51.851%

Left genome: K(OR)GR(GYGYGY)GRGY(YR)YYRG(RO)YY(OR)RK

Tay 18

Fuzzy, Mottled Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 152/Ava 153) Observed:

Left Spoint: 0 seeds

Right Splint: normal

Left splint had (RO) broken, Right splint had junk swapped.

Solvents on Right Splint showed:

• ORYY
• RRRO pinpoints it as the G right before the (RO) gene, being swapped to an R.

Right genome: K(OR)GR(GYGYGY)GRGY(OR)YYRR(RO)YY(OR)RK

gene targeted was 20/27

gene range is 19/27 to 20/27 or 70.370% to 74.074%

Left Splint had R in (RO) gene swapped to a G.

Left genome: K(OR)GR(GYGYGY)GRGY(OR)YYRGROYY(OR)RK

gene targeted was 21/27

gene range is 20/27 to 21/27 or 74.074% to 77.777%

Tay 19

Green, Mottled, Spotted Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 156/Ava 157) Observed:

Left Spoint: no water, 1 flax (first, second, fifth or sixth gene in (GYGYGY) sequence broken)

Right Splint: 3 weed (OR) broken

Solvent on Left:

• GROY
• OYYO
• RYYR
• ORYY
• RGRG
• RGYO
• YORY
• YYGR
• GRGO
• GYOR
• GRGR either 1st or 3rd Y in (GYGYGY) replaced with R. Can't tell, the symmetry of the genome is big. Would need a very large solvent.

So either 6th or 10th gene was targeted.

(determined from TEST 2 that it was 10th gene, see below for more details)

10th gene of 27

genome is K(OR)GR(GYGYGR)GRGY(OR)YYRG(OR)YY(OR)RK

gene range is 9/27 to 10/27 or 33.333% to 37.037

Right Splint:

• YGYG
• GRGY
• OYRK <-- pinpoints as the 26th gene, R --> Y

Left genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OY)RK

gene targeted is 26/27

range is 25/27 to 26/27 or 92.592% to 96.296%

TEST 2: Energy / Silken

Silken should donate an I. This will test to see if it was gene 6 or gene 10 in Test 1 that was swapped.

if it was gene 6, then gene 8,9,10 or 11 will swap out of Energy (UYYY), inner East Yellow gene set

if it was gene 10, then gene 14, 15, 16, or 17 will swap out of Energy (GYYY), Outer south yellow gene set

Mutagen Applied: Ava 158/Ava 159 Observed:

RIght has IY gene set now from color observations. therefore, it was either 17th or 18th gene of 18 in Silken that swapped.

Can't tell more w/o solvent testing.

Left has IY gene added and lost Yellow on south petal.

therefore, it was gene 10 that was swapped in test 1. That test information has been updated.

furthermore, because the IY is present, we know (GYYY) --> GYIY.

here, gene 16 of 42 was swapped.

genome is VVVVV UYYY UYYY GYIY GYYY GRRO GRRO UROR UROR VVVVV

gene range is 15/42 to 16/42 or 35.714% to 38.095%

new Left range is 35.714% to 37.037

TEST 3: Vampire/vampire (bessodia's test)

Left: hit gene 5.

range is 4/27 to 5/27 or 33.333% to 41.666%

this is consistent w/ previous range

Right: hit gene 12

range is 11/12 to 12/12 or 91.667% to 100%

this is consistent w/ previous range

TEST 3: Vamp/Crown (Ava 234, Ava 235)

expected Vamp to donate gene 5, I

should hit gene 35 or 36 on crown.

if gene 35, Yw would break, and gain IO

if gene 36, Yw would break and gain IR

Right side: Yw broke and IO was added

therefore, gene 35/ 37 was targeted

range was 91.891% to 94.594%.

New right range is 92.592% to 94.594%

Tay 20

Retic, Smelly, Striped Renard opened

TEST 1: Nile Green / Nile Green (Ava 228, Ava 229) Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

observed: no change in phenotype.

left solvents:

• OYYO
• KORG
• RYYR
• RGRY
• YGRG
• YORY
• GRGY
• GYGY
• RGRO
• GYGR
• YRGR
• YORR

Looks like same gene was swapped. This test is inconclusive :(

Tay 21

Dry, Spotted Renard opened

TEST 1: Nile Green / Nile Green (Ava 232, ava 233) Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

Observed: Left had no water, 2 weeds, and 1 flax

right had no water, 2 weeds, and no flax

Left must have hit outer GY's in (GYGYGY)

Solvents showed: *GYGY

• RYYR
• GRGY
• YGRG
• YGYY --- pinpoints as G--> Y, 9th gene

gene 9/27 targeted

gene range is 8/27 to 9/27 or 29.629% to 33.333%

Right must have hit inner GY in (GYGYGY)

SOlvents showed:

• RGRO
• ORRK
• YORY
• YGRG
• RGRO
• GYGR
• ORYY
• OYYO
• GYGR
• ROYY
• GROY
• GGYG <-- pinpoints as the 8th gene, Y --> G

gene 8 of 27 targeted

gene range is 7/27 to 8/27 or 25.925% to 29.629%

TEST 2: crown/clarity (Ava 236, ava 237)

expect: gene 11, 12, 14 on crown (O,O,V) and gene 10, 11 in clarity (Y,I)

observe: lost IY on clarity. Crown broke Mw.

Right: must be an I-->O. (the O is from crown, Mw breaking means it wasnt' a V gene)

(also, if it was a Y on right, clarity would have gained an IO, which it did not).

gene is 11/36

range is 10/36 to 11/36, or 27.777% to 30.555%

new Right range is 27.777% to 29.629%

Left: knew it had to be an O gene. Knowing now that an I was swapped, gene 11 could not have been targeted on left, or an IO would have resulted.

therefore, gene 12/37 was targted.

new Left range is 11/37 to 12/37, or 29.729% to 32.432%

Tay 22

Dry, Spongg Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

Observed no change in either side.

Either junk genes swapped or same gene swapped.

Left splint Solvents:

• YYRG
• RGRO
• ORYY
• GROY
• GRGY
• GYGY
• GYGR
• YGRG
• YORR

all but one junk gene covered ((14th gene)

presuming it was same gene swapped, so this test is inconclusive.

Tay 23

Dry, Phos, Slimy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

Observed: both splints had one extra weed step,

Therefore, both broke a (OR) gene

Left Solvents:

• RGYO
• YORY
• ORYY
• OYYR pintpoints as the O in last (OR) gene set.

gene 25 of 27 was targeted

range is 24/27 to 25/27, or 88.888% to 92.592%

RIght solvents:

• GROY
• GRGY
• RGRO
• OYYO
• KOOG <-- pinpoints as the 2nd gene, R--> O

gene 2 of 27 was targeted

gene range is 1/27 to 2.27, or 3.703% to 7.407%

TEST 2: vamp/blush (Ava 248, 249)

vamp will donate 11th gene (I)

blush will hit gene 1 or 2

observed that blush gained an IO, so it hit gene 2 (GROR --> GIOR)

targeted gene 2 of 26

range is 1/26 to 2/26, or 3.846% to 7.692%

new right range is 3.846% to 7.407%

TEST 3: vamp/silk (Ava 246, 247)

expected vamp to donate 11 or 12 (R,O); Silken donate 1 or 2 (both Rs)

observed: vampire lost magenta and gained a cyan on outer stamen

therefore, gene 11 of 12 was targeted (IO -->IR)

left gene range is 10/12 to 11/12 or 83.333% to 91.666%

new left range is 88.888% to 91.666%

can't get better info on right splint w/o solvents.

Sterope Recipes

Sterope 02

Crackly, Reticulated, Slimy Aryballa opened

TEST 1: Blush/Crown (gives Ava 168, Ava 169)

Expected: Left to hit gene 16 or 17, Right to hit gene 2 or 3.

if 16/2, blush loses IO, crown gains IOO and breaks Mw

if 16/3 blush breaks IO, crown gains IO, breaks Mw

if 17/2, blush breaks IO, crown breaks Mw

if 17,3, O swaps with O, no changes.

Observed:

no changes!!

Therefore, left splint hit gene 17/26

range is 16/26 to 17/26 or 61.538% to 65.384%

new range is 63.334% to 65.384%

Right target was 3/37

right range is 2/37 to 3/37, or 5.405% to 8.108%.

this is consistent w/ previous tests.

TEST 2: Dusk/Ava 26 should swap the 1st R from Ava 26. (if not, next Gene is an R too)

for Left splint, 4 possibilities:

47th gene: GROR --> GRRR north magenta broken, South Cyan gained 48th gene: GROR --> GROR no changes, same gene swapped (R) 49th gene: IOOO --> ROOO broke magenta on outer, inner and middle stamen 50th gene: IOOO --> IROO broken magenta on outer, inner and middle stament, gained cyan on outer stamen

Observed:

Left splint (Ava 99) looks exactly like Dusk

Right splint (Ava 100) is all white.

Therefore, the 48th gene was swapped from Left splint.

48/73 gene

range is now 47/73 to 48/73 or 64.383% to 65.753%

Sterope 03

Dry, Fuzzy, Slimy Aryabella opened

Test 1: Vampire/Vampire (Bessodia's tests)

Left splint hit gene 8

gene range is 7/12 to 8/12 or 58.333% to 66.666%

Right splint hit gene 1

gene range is 0/12 to 1/12 or 0% to 8.333%

Test 2: Morning/vampire (Ava 127, Ava 128)

Left targeted gene 29, G-->I

gene range is 28/44 to 29/44 or 63.636% to 65.909%

right targeted gene 1, I -->G

gene range is 0/12 to 1/12, same range as test 1

Sterope 04

Crackly, Dry Reticulated Sabahl opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 88/Ava 89)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• ORYY
• GRGY
• YORY
• ORRK
• RGYO
• OYYO
• RGRO
• RYYR
• RGYG
• GROY

no anomolies found yet. Standard Junk genes 4/27 and 11/27 not covered conclusively.

Solvents on Right Splint showed:

• RGYG
• ORRK
• RGYO
• YRGR

no anomolies found yet. Starting to think this mutagen swapped same genes.

this test is inconclusive (could go back to solvents more)

Sterope 05

Phosphorescent, Prickly, Slimy Sabahl opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 118/Ava 120)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• YYOR
• YGRG
• RGYG
• GYOR
• YORR
• YORY
• RGYO
• KORG
• RGRG
• RGRO
• ORGR
• GRGY
• YGYG
• YYOR
• OYYO

no anomolies found yet. Standard Junk genes 18/27 not covered conclusively. Seems to be that the same genes were switched.

Sterope 06

Calico, Crackly, Spongy Avanya opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 117, Ava 118) Observed:

Left Spoint: 1 more weeding

Right Splint: 2 seeds

Left splint had lost an (OR) gene Right splint had gained (RO) gene

Solvents on Left Splint showed:

• GYGY
• RGYY <-- that pinpoints it as the second (OR) --> YR

Left Genome: K(OR)GR(GYGYGY)GRGYYRYYRG(RO)YY(OR)RK

gene targeted was 15/27.

gene range is 14/27 to 15/27 or 51.851% to 55.555%

also reveals that a "O" gene was moved to the left splint

Solvents on Right Splint:

• ORGR
• YORR
• RGYO
• GRGY
• ROYR --> points to the 17th gene Y --> O.

Right sGenome: K(OR)GR(GYGYGY)GRGY(OR)OYRG(RO)YY(OR)RK

gene targeted was 17/27

gene range is 16/27 to 17/27 or 59.259% to 62.962%

TEST 2: Ava 29/Ava 29 (Clarity, Clarity, really)

Left: will target gene 19, 20, or 21. (GROR)(GRRO) (thats ORG in middle) Right: will target gene 22 or 23, both R's in (GRRO)

Observed: Left Splint (Ava 144) gained Cyan South (GRRR) and lost magenta North.

that means gene 19 was targeted.

range is 18/36 to 19/36 or 50.000% to 52.777%

combining with test 1 results, the new range is 51.851% to 52.777%

Right Splint (Ava 145) gained an O gene. Dont know where w/o using a solvent.

Celano Recipes

Celano 01

Dry, Spongy, Spotted Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 90/Ava 91)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• RGYO
• GYGR
• YYRG
• GYOR
• KORG
• YORY
• YGYG
• GYGY
• ORGR
• GROY
• RYYR
• RORG
• YGRG

covered all of the genome except the last 4 genes. Not showing anything yet, so stopping to save solvents... no conclusions drawn yet

Celano 02

Green, Mottled, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 92/Ava 93) Observed:

Left Spoint: normal

Right Splint: 2 weed, 1 flax 1 water.

Left splint had no gene groups broken Right splint had (GYGYGY) broken (but not middle GY)

Solvents on Right Splint showed:

• RYYR
• YYYG <-- that pinpoints it as the third G directly in(GYGYGY) as being replaced with an Y

Right Genome: K(OR)GR(GYGYYY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 9/27.

gene range is 8/27 to 9/27 or 29.629% to 33.333%

also reveals that a "G" gene was moved to the left splint

Solvents on Left Splint:

• GYGY
• GYGR
• RGYR --> points to the 17th gene Y-->G.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)GYRG(RO)YY(OR)RK

gene targeted was 17/27

gene range is 16/27 to 17/27 or 59.259% to 62.962%

TEST 2: Ava 24/ Dusk (actually Ava99, which is exactly Dusk genome)

gives Ava 135 / Ava 136

expect: Left splint will donate gene 11 or 12. both are G's. Ava 24 genome: RRROOOYYGGGGUUUIII

Right Splint: should be gene 23 to gene 26: (gene set IYYY) if 23, IYYY --> GYYY lose yellow on entire stamen, gain S yellow if 24, IYYY --> IGYY lose yellow on entire stamen if 25, IYYY --> IYGY lose yellow on inner and middle stamen if 36, IYYY --> IYYG lose yellow on inner stamen.

Observed: Ava 136 looks like Dusk but with yellow on South petals, and one less yellow shade on all stamen parts. therefore, gene 23 of 73 was targeted! genome: GRORGORRUYYYGYORIYGRROGYYYGRROGORRGRORROYGIOGRORIOOOIYYIYURRRGRROGORRUOOO range is 22/73 to 23/73, a new range is 30.136% to 31.506%

Left splint needs solvent to determine which gene was switched.

Celano 03

Calico, Reticulated, Smelly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Ava 160/Ava 161 Observed:

Left: normal + 1 Rotten flax (made RRGY gene set)

Right: no seeds (RO) was broken

Right Splint:

since we made an RR in the other gene, we know the right splint donated an R.

since we broke the only RO gene set, it was the 21st gene targeted.

Right genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(GO)YY(OR)RK

range is 20/27 to 21/27 or 74.074% to 77.777%

(the fact that it was a G gene swapping in is from Left Splint info)

Left Splint: solvent showed:

• ORYY
• YGYG
• ORRK
• RRGY
• ROYY
• YRGR
• GRGY
• YGYR <-- pinpoints as the G right after the (GYGYGY) set that was switched to R

Gene targeted was 11th of 27, G --> R

genome is :Right genome: K(OR)GR(GYGYGY)RRGY(OR)YYRG(RO)YY(OR)RK

range is 10/27 to 11/27 or 37.037% to 40.740%

TEST 2: Crown / vampire (Ava 184, Ava 185)

Expected gene 14, 15, 16 on crown (U, R, V) and gene 9, 10 on vampire (I,O)

Observed: no visible change on cornw (but we were targeting junk genes)

vampire broke mageneta, IO , as expected.

Cannot tell more w/o solvents.

Celano 04

Dry, Phos, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 164/Ava 165) Observed:

Left Spoint: normal

Right Splint: normal

either in junk genes or same gene was swapped.

Solvents on Left Splint showed:

• ORRK
• YYOR
• YGRG
• RGYO
• ORGR
• GRGY
• RYRO <-- that pinpoints it as the G right before the (RO) gene set

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRY(RO)YY(OR)RK

gene targeted was 20/27.

gene range is 19/27 to 20/27 or 70.370% to 74.074%

Solvents on Right Splint showed:

• RGRG
• ROYG <-- that pinpoints it as the second Y after the (RO) gene set

RightGenome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YG(OR)RK

gene targeted was 24/27.

gene range is 23/27 to 24/27 or 85.185% to 88.888%

TEST 2: delicate / fracture (Ava 224, Ava 225)

Expect: fracture to hit at gene 16/18 (center O in GOOO)

delicate to hit gene 12 or 13

if 12, lose IY --> IO

if 13, lose dwarf gene, no color change (GYOR --> OYOR)

Observed: delicate lost IY, gained IO, and fracture is all white now.

therefore, Left splint hit gene 12

range is 12/18 to 13/18 or 68.750% to 75.000%

that is within the previous range.

(right splint also checked, still within the range)

Celano 05

Calico, Phos, Spotted Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 196/Ava 197) Observed:

Left Spoint: 2 weeds (broke an (OR)

Right Splint: normal (had junk gene swapped)

Solvents on Left Splint showed:

• GRGY
• GYGR
• RGRO
• YRGR
• RRGR <-- that pinpoints it as the first gene, O --> R

Left Genome: KRRGR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 1/27.

gene range is 0/27 to 1/27 or 0% to 3.703%

also reveals that a "O" gene was moved to the right splint

Solvents on Right Splint:

• GRGY
• YGRG
• ROYY
• YGYG
• GYGY
• ORYY
• ORGR
• OYYO
• KORG
• YOGR --> points to the 19th gene R --> O.

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYOG(RO)YY(OR)RK

gene targeted was 19/27

gene range is 18/27 to 19/27 or 66.666% to 70.370%

Alcyone Recipes

Alcyone 01

Calico, Crackly, Pricky

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 105/Ava 106) Observed:

Left Spoint: had an extra weed step (OR) was broken

Right Splint: no change, must have mutated junk

Solvents on Left Splint showed:

• GYGY
• GYGR
• GRGY
• YRGR
• OGYY <-- that pinpoints it as the 4th R being replaced with a G.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 16/27.

gene range is 15/27 to 16/27 or 55.555% to 59.259%

also reveals that a "G" gene was moved to the left splint

Righgt Splint:

The right gene results show that an "G" gene was switched w/ a "R" gene, in the junk sections

Solvents showed:

• KORG
• YORY
• RGRG
• YGYG
• YYRG
• ORYY
• YORR
• GRGY
• RYYR
• YYOR
• ORRK
• YGRR <--- pinpoints as 6th G being replaced w/ an R.

Right Genome: K(OR)GR(GYGYGY)GRRY(OR)YYRG(RO)YY(OR)RK

gene targeted was 13/27

gene range is 12/27 to 13/27 or 44.444% to 48.148%

TEST 2: Vampire / Vampire (bessodia's test) left result is gene 7/12

range is 6/12 to 7/12 or 50.000% to 58.333%

that means the new left range is 55.555% to 58.333%

right result is gene 6/12

range is 5/12 to 6/12 or 41.666% to 50.000%

that range is consistent w/ the above results.

TEST 3: blush/morning (Ava 172, ava 173)

Expected: blush should hit gene 15, 16 (VI), Morning should hit gene 20-22 (YUR) Observed: blush lost IO, so gene 16 was targeted! morning lost Ywest and gained IR, so gene 21 was hit

Left gene is 16/26

left range is 15/26 to 16/26 or 57.6925 to 61.538%

new left range is 57.692% to 59.259%

Right gene is 21/44

Right range is 20/44 to 21/44 or, the new range of 45.454% to 47.727%

TEST 4: crown/blush (Ava 178, Ava 179)

Expected: gene 22 or 23 on crown (V O) and gene 12 or 13 on blush (R O)

Observed: crown gained MW: URVO --> UROO

Observed: blush lost Mw

Left: gene 22 was targeted!

gene is 22/37

range is 21/37 to 22/37 or 56.756% to 59.459%

this is consistent

Right: gene 13 of 26 was targeted.

range is 12/26 to 13/26 or 46.153% to 50.000%

the new right range is 46.153% to 47.727%

AC1 02

Green, Phosphorescent, Smelly Avanya opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 131/Ava 132)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• YYOR
• RYYR
• GYGY
• YGRG
• GRGY
• YORR

no anomolies found yet. Standard Junk genes mostly covered

this test is inconclusive. Could do further solvent testing, but at this point, no results.

TEST 2: Ava 29/Ava 29. (Ava 29 has 4 junk genes, otherwise, its just a clarity bulb) Ava 26 genome: IRIRIOIOIYIYGORUGRORGRROGORRGRORGRRO

mutagen applied: gave Ava 142 / Ava 143

Right Splint: observed: north petal lost a cyan (now is 2 red shades), and outer stament gained 1 cyan (+IR)

The GORR gene set was targeted (to break North cyan) and the first R was switched to an I, to give GOIR.

Right Genome: IRIRIOIOIYIYGORU GROR GRRO GOIR GROR GRRO

27th gene of 36 was targeted

range is 26/36 to 27/36 or 72.222% to 75.000%

Left Splint: observed outer stamen lost 1 yellow an IY gene was targeted, and it lost the I.

so IYIY became either RYIY or IYRY

either 9th or 11th gene targetd

range is 8/36 to 11/36 or 22.222% to 30.555%

could narrow this down by testing w/ solvents

TEST 3: vampire/energy (gave Ava 166, Ava 167) expect gene 3 or 4 of vampire, and gene 31 or 32 of energy

if gene 3 and gene 31, energy loses yellow west and gains IO

if gene 3 and gene 32, energy loses Yw and gains IR

if gene 4 and gene 31, energy loses Yw Vampire gains IR

if gene 4 and gene 32, energy loses Yw. Vampire gains IO

Observed: energy lost Yw. STamen has no color. Vampire got an extra magenta on outer stamen

therefore, left splint hit gene 4

range is 25.000% to 33.333%

new range is 25.000% to 30.555%

right splint hit gene 32 out of 42

range is 31/42 to 32/42 or 73.809% to 76.190%

new range is 73.809% to 75.000%

AC 03

Hairy, Slimy Avanya opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 208/Ava 209)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• YYOR
• OYYO
• YYRG
• YGRG
• YGYG
• RGYO
• RYYR
• RGYG
• RGRG
• ORYY
• YORR

no anomolies found, and standard junk genes all covered.

The same gene types were swapped between splints

this test is inconclusive

Crosses for Lily Requirements

Aiming for 75% Green petals

Stragety: Add cyan to one set of inner petals on Morning, then break one of the two yellow genes for that same set. This will turn that inner set of petals green. The outer two sets of petals are already green, so that will be 3/4 green.

CROSS 1: Morning / Crown with calico,dry, spotted (Sterope 01) Aiming to swap first genes of both splints. Unmutated Morning:

K(IRRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Cyan1OSMSIS(IRRR)+Yel2OSMSIS(IYYY)+Yel2IEP(UYYY)+Yel2IWP(UROR)+Cyan1OSP(GRRR)+Yel1OSP(GYYY)+Cyan1ONP(GORR)+Yel1ONP(GRRO)

Unmutated Crown:

K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Red3=[Mag3IWP(UROO)+Ye3IWP(UROR)] + 5V

This mutagen will Turn (IRRR) (cyan on stamen) into (URRR), cyan on Inner East Petal.

Applied mutagen. (Ava 45/Ava46) Observed:

Left Spoint(Ava 45): Inner east petals now greenish yellow. the cyan add was a success!!

Genome of Ava 45: K(URRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Right Splint(Ava 46): Inner petals look orange, not red

Genome of Ava 46: KIROO(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Right splint had first gene broken, UROO (magenta inner petals) --> IROO upper stamen cyan 1. inner petal set now 3 yellow shades + 2 magenta shades = red + red + yellow, or a dark orange

CROSS 2: Ava45 / Blush with Fuzzy, Hairy, Spongy (Tay 06)

aiming to break up a (UYYY) inner yellow gene on Ava 45

Mutation should swap gene 18/44 (Y) on Ava 45 with gene 10/26 (V) blush. Unmutated Ava 45: K(URRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K Unmutated Blush: K(GROR)V(GOOO)V(UROO)V(IO)V(IOO)V(IOOO)K

Ava 47 / 48 resulted. Observed: left splint (Ava47) does have predicted colors. Green on outside petal sets. one green and one yellow inner petal sets. right splint (Ava48): colors appear to be blush. No observed change.

Hypothesized Ava 47 genome:

K(URRR)(IYYY)(IYYY)(UYYY)UYVY(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Hypothesized Ava 48 Genome:

K(GROR)V(GOOO)Y(UROO)V(IO)V(IOO)V(IOOO)K

both are consistent with expected and observed results

Aiming for Cyan on Inner Stamen

Maia needs this flower for Xenobotany

Test 1: morning/vampire (Ava 127, Ava 128) Mutagen Sterope 03

Expected: should convert a "GRRR gene to IRRR", which would be the second IRRR gene in morning. Vampire should lose one IY, since it is donating an I, turning it into GY.

Observed: results are as expected. Vampire has 3 magenta 2 yellow on outer stamen, instead of 3 mag, 3 yellow. Right genome: GYIYIOIYIOIO

Morning lost some cyan on south petal, but inner stamen is now two shades of green. Left genome: IRRR IYYY IYYY UYYY UYYY UROR UROR IRRR GYYY GORR GRRO

tried to donate to Univ., but they said its not acceptable. That means probably can't have any OTHER inner stamen colors. Next step is to remove the two yellow shades then

Test 2: vampire/Ava 127 Tay09 (Calico, Green Smelly)

Vampire should donate 6th gene, O. Ava 127 will hit either gene 9, 10, 11 in IYYY

• if 9, I in IYYY will be targeted --> OYYY removing 1 yellow from entire stamen
• if 10, first Y in IYYY will be targeted --> IOYY removing 1 yellow from entire stamen and gaining magenta on outer stamen
• if 11, second Y in IYYY will be targeted --> IYOY removing 1 yellow from middle and inner stamen

Mutagen applied, giving Ava 154 / Ava 155 Observed: Left Splint, Ava 154 has gained yellow on outer stamen. It was a Y it gained. genome is: IYIOIYIYIOIO

Right Splint, Ava 155 lost yellow on entire stamen and gained magenta on outer stamen.

Therefore, 10th gene was targeted.

Genome is IRRR IYYY IOYY UYYY UYYY UROR UROR IRRR GYYY GORR GRRO

range is new for RIght splint

range 9/44 to 10/44 or 20.454 to 22.727%

first yellow shade was removed successfully

Test 3: Ava 144 / Blush mutagen Tay 12 (Calico, Crackly, Prickly)

Blush will donate either gene 13 or 14, either is an O Ava 155 will have gene 6, 7 or 8 targeted. The Y's in IYYY

• if gene 6, IYYY --> IOYY
• if gene 7, IYYY --> IYOY
• if gene 8, IYYY --> IYYO

Mutagen applied, giving Ava 162/Ava 163

Left splint lost yellow on inner and middle stamen. outer stamen unchanged.

therefore, gene 7 was targeted.

range is 6/44 to 7/44 or 13.636% to 15.909%.

new min range for this mutagen!

Genome is IRRR IYOY IOYY UYYY UYYY UROR UROR IRRR GYYY GORR GRRO

second yellow shade was removed successfully

University Accepted!!!!