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− | Originally from the Bastet server, I transfered to Tale 5 only a few months ago with some friends. (Now we form the Bastet Hounds Guild). | + | Originally from the Bastet server, I transfered to Tale 5 with some friends. (Now we form the Bastet Hounds Guild). |
| My primary passion in Egypt is glassblowing and glassmaking. I also enjoy the Test of Pyrotechnics, Thought puzzles, and crossbreeding flowers. | | My primary passion in Egypt is glassblowing and glassmaking. I also enjoy the Test of Pyrotechnics, Thought puzzles, and crossbreeding flowers. |
| I am always open to helping people in any way that I can, so please chat me anytime. | | I am always open to helping people in any way that I can, so please chat me anytime. |
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| This includes: | | This includes: |
| *Mushrooms (Sand Spore, Slaves Bread, Razor's Edge, Peasant Foot, and Salt Water Fungus). | | *Mushrooms (Sand Spore, Slaves Bread, Razor's Edge, Peasant Foot, and Salt Water Fungus). |
− | *Cobra Skins (as many as possible)
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| | | |
| I am also always happy to purchase | | I am also always happy to purchase |
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| I am hoping to help Egypt make better use of its Portable Star Labs. I see so many of them laying around unused, or used as decorations. They are meant to be used and returned to a university of Art (preferably Sterope). I have a Law Petition circulating to help us use them more effectively. Please remember they are used for opening new Star recipes only...they are NOT part of the Principle or Test of Pyrotechnics!! They are given out in a limited amount, so they are a precious resource. Please see http://www.atitd.org/wiki/tale5/Portable_Star_Lab if you have any questions, or chat me in game. | | I am hoping to help Egypt make better use of its Portable Star Labs. I see so many of them laying around unused, or used as decorations. They are meant to be used and returned to a university of Art (preferably Sterope). I have a Law Petition circulating to help us use them more effectively. Please remember they are used for opening new Star recipes only...they are NOT part of the Principle or Test of Pyrotechnics!! They are given out in a limited amount, so they are a precious resource. Please see http://www.atitd.org/wiki/tale5/Portable_Star_Lab if you have any questions, or chat me in game. |
| | | |
− | ==Mutagenics Research:==
| + | In addition, I am happy to help anyone with passing the principle of Pyrotechnics. If you have questions how to use a mortar, make stars, or anything pyro related, please chat me. If I can't help, I can probably direct you to someone that can! |
− | I am trying to help characterize the mutagens that are being researched. | |
− | Specially, I'm trying to determine which gene ranges are swapped from the left and right splints.
| |
− | I won't bore you here with genetics theory, it is all explained much more clearly elsewhere on the wiki. | |
− | But I would like to make my data available to others that share this interest.
| |
| | | |
− | This is my first time doing this, so PLEASE chat me if you think I am doing something wrong. Right now, our guru's of genetics are not playing, so I don't have a good source to verify this with. I welcome all input!!
| + | ==Mutagenics Research== |
| + | You can find my test data for mutagens at the following page: |
| + | http://www.atitd.org/wiki/tale5/User:Avanya/mutagenics |
| + | My most recent data is not on this page, because it got too long to put it all on there, lol. But if you are interested, please chat me, and I can get you copies of data from my notes. |
| + | ==Test Ideas== |
| + | [[Test of Gemology]] |
| | | |
− | :'''I am not sure how the mutagen range rounds'''.
| + | [[Test of Sponsorship]] |
− | For example, if a mutagen range is that it will swap a gene at 10%. Given a genome of length 16, 10% along is between gene 1 and 2. Does it pick the closer gene (in that case, gene 2) or is it a floor rounding function? (that would truncate the extra %, and round down to gene 1). Or does it pick randomly between the two genes? Knowing this is important! I am assuming here it picks the closer gene, but further tests might reveal the actual rounding method.
| |
| | | |
− | ==Tayete Recipes==
| + | [[Test of Magnificent Gardens]] |
− | ===Tay 01===
| |
− | Dry, Prickly, Reticulated Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 41/Ava 42)
| |
− | Observed:
| |
− | :Left Spoint: 1 weed/1 water/1 seed/2 flax/<span style="color:red;">1 rotten flax</span>
| |
− | :Right Splint: 0 zeed!!
| |
− | | |
− | Right splint had no gene groups broken, but gained an additional gene sequence of RRGY
| |
− | Left splint had the (RO) gene group broken.
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *ORR
| |
− | *GYG
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− | *YRR <-- that pinpoints it as the third G directly after (GYGYGY) as being replaced with an R
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− | | |
− | Right Genome: K(OR)GR(GYGYGY)<span style="border:1px solid green;">R</span>RGY(OR)YYRG(RO)YY(OR)RK
| |
− | :gene targeted was 11/27.
| |
− | :gene range is 10.5/27 to 11.5/27 or <span style="color:red;">38.889% to 42.593%</span>
| |
− | :also reveals that a "G" gene was moved to the left splint
| |
− | | |
− | :Left Splint:
| |
− | :The phenotype revealed that the (RO) seed gene was mutated.
| |
− | :The right gene results show that an "R" gene was switched w/ a "G" gene.
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− | | |
− | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(<span style="border:1px solid green;">G</span>O)YY(OR)RK
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− | :gene targeted was 21/27
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− | :gene range is 20.5/27 to 21.5/27 or <span style="color:red;">75.926% to 79.630%</span>
| |
− | </div>
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: Fracture / Crown (Ava 43,44)
| |
− | :phenotypes: Fracture became giant, colors unchanged. Crown petals went from red to magenta
| |
− | | |
− | :Left splint: Fracture: K(ROYG)(ROY(G)YOR)GOR(GOOO)K
| |
− | :Test 1 predicts the 1st Y in the "ROYGYOR" set will be swapped. if so, it should be a 1 giant + 1 dwarf = small size.
| |
− | :observed: its a giant. no color changes
| |
− | To be a size 1 giant, the both 1 giant and 1 dwarf gene must have been disabled.
| |
− | This would be accomplished if the shared "G" were swapped.
| |
− | This is just outside the predicted range of TEST 1. Perhaps I am not rounding out enough?
| |
− | | |
− | :Right splint: Crown: K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K
| |
− | :Test 1 predicts that gene 28 or 29 will swap. (O or 2nd R in the first (UROR) set. If so, a yellow gene will be broken.
| |
− | :observed: the petals are more magenta colored than red. This is consistent w/ above prediction. It could also be a gene before or after this one that moved, and give the same results, so the exact gene position isn't certain w/o solvent testing.
| |
− | | |
− | Hypothesized Ava 43 genome:
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− | :K(ROYG)ROY(<span style="border:1px solid green;">R</span>O)YORGOR(GOOO)K
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− | gene 8/18 targeted.
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− | 7.5/18 to 8.5/18 range is 41.667% to 47.222% for left splint target
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− | | |
− | Range overlap is <span style="color:red;">41.667% to 42.593%.</span>
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− | | |
− | </div>
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− | | |
− | <div style="margin:30px">
| |
− | TEST 3: Dusk / Fracture (Ava 72, 73)
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− | :phenotypes: no changes were observed !!
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− | | |
− | Expected Left: gene 30 or 31. If 30 (GRRO) --> (GRRR)removing Yellow north and adding cyan south
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− | If 31 (GORR) --> RORR, breaking Cyan North.
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− | | |
− | Expected Right--> 14th gene, R from GOR to swap in. GOR is junk, so no phenotype change is expected.
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− | | |
− | Results: Either its gene 29 from Dusk that moved, R --R so no genome changes resulted
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− | then left range is 28.5/73 to 29.5/73 or 39.041% to 40.411%
| |
− | | |
− | OR
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− | | |
− | gene 14 from Fracture did not swap. (if gene 15 did, and gene 31 on Dusk, it would also be no change in genome... G-->G)
| |
− | Solvents are needed to resolve this further.
| |
− | </div>
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 4: Vampire / Vampire (Bessodia's Test)
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− | | |
− | Vampire genome is: KIYIYIOIYIOIOK 12 genes long
| |
− | Left splint:
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− | *IYIOI
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− | *YOOIY
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− | *YIOIO
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− | *YIYIO
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− | *OOIYI
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− | *IYOOY
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− | *OIOK
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− | *KIYIY
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− | *IYIYO
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− | *YIYOO
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− | | |
− | an I gene swapped for an O gene:
| |
− | KIYIY<span style="border:1px solid green;">O</span>OIYIOIOK
| |
− | 5th gene was targeted.
| |
− | range is 4.5/12 to 5.5/12 or 37.500% to 45.833%
| |
− | that's consistent w/ the other test results!
| |
− | | |
− | Right splint:
| |
− | *IOIY
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− | *IYIO
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− | *YIII
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− | *IYII
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− | *IIIO
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− | *IYIY
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− | *IIOK
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− | *KIYI
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− | *IIIO
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− | *OIYI
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− | | |
− | genome is: KIYIYIOIYI<span style="border:1px solid green;">I</span>IOK
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− | 10th gene was targeted.
| |
− | range is 9.5/12 to 10.5/12 or 79.167% to 87.500%.
| |
− | that narrows the range down, when combined w/ other tests
| |
− | so the range is now <span style="color:red;">79.165% to 79.630% </span>
| |
− | </div>
| |
− | | |
− | ===Tay 02 ===
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− | Calico, Green, Striped Renard opened
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− | | |
− | <div style="margin:30px">
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− | TEST 1: Nile Green / Nile Green
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− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
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− | ::1 weed/1 water/1 seed/2 flax/0 rotten
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− | | |
− | applied mutagen.
| |
− | Observed:
| |
− | :both new splints had same phenotypes. (Ava 37/Ava 38)
| |
− | | |
− | Both splints had junk genes swapped, or same type of gene swapped.
| |
− | | |
− | Solvents on Right Splint showed:
| |
− | *RGRO
| |
− | *GRGY
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− | *ORYY
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− | *ROYY
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− | *GROY
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− | *GRG
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− | *GYOR
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− | *YORY
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− | *YGYG
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− | no anomolies found yet. Standard Junk genes all covered
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− | | |
− | :Solvents on Left Splint showed:
| |
− | *GRGY
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− | *ORYY
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− | *YGRG
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− | *GROY
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− | *RGRO
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− | *ORGR
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− | *GYGY
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− | *YGYG
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− | *GYOR
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− | *YRG
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− | *ORRK
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− | | |
− | no anomolies found, and standard junk genes all covered.
| |
− | :this test is inconclusive
| |
− | </div>
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: Silken / Vampire (Ava 26, 27)
| |
− | :phenotypes: Silken bulb unchanged, Vampire had center go magenta (ie had an IO get broken)
| |
− | | |
− | :Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK
| |
− | :Right splint: Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K
| |
− | Left Splint:
| |
− | *IIIK
| |
− | *RRRO
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− | *UIII
| |
− | *YYYG
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− | *YYYY <-- pinpoints as the last O --> G
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− | genome is KRRROO<span style="border:1px solid green;">Y</span>YYYGGGUUUIIIK
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− | 6th out of 18 gene targeted
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− | range is 5.5/18 to 6.51/18 which is <span style="color:red;">30.556% to 36.111%</span>
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− | | |
− | Right Splint:
| |
− | know from phenotype we lost an IY, know from above we had Y-->O. so we trade IY for IO.
| |
− | *YIOI
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− | *KIYI
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− | *IOIO
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− | *IOIYI <-- pinpoints as the second Y
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− | genome is KIYI<span style="border:1px solid green;">O</span>IOIYIOIOK
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− | 4th gene out of 12
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− | range is 3.5/12 to 4.5/12 which is <span style="color:red;">29.167% to 37.500%.</span>
| |
− | | |
− | </div>
| |
− | | |
− | | |
− | <div style="margin:30px">
| |
− | TEST 3: Vampire/ Vampire (Bessodia's Test)
| |
− | Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K
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− | | |
− | no visual different between the flowers.
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− | solvent testing showed no changes.
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− | presuming the same gene type swapped.
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− | No conclusions available.
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− | | |
− | | |
− | </div>
| |
− | | |
− | ===Tay 03 ===
| |
− | Calico, Mottled, slimy Renard opened
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− | | |
− | <div style="margin:30px">
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− | TEST 1: Nile Green / Nile Green (Ava 35, Ava 36)
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− | Unmutated Genome:
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− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
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− | ::1 weed/1 water/1 seed/2 flax/0 rotten
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− | | |
− | applied mutagen.
| |
− | Observed:
| |
− | :Left Splint: 1 weed/1 water/1 seed/2 flax/0 rotten
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− | :Right Splint: 1 weed/01 water/1 seed / 2 flax/ 0 rotten
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− | | |
− | Both splints had junk genes swapped.
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− | | |
− | Solvents on Right Splint showed:
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− | *GGYG <-- that pinpoints it as 12th gene, R being replaced with G
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− | | |
− | Right Genome: K(OR)GR(GYGYG)G<span style="border:1px solid green;">G</span>)GY(OR)YYRG(OR)YY(OR)RK
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− | :gene targeted was 12/27.
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− | :gene range is 11.5/27 to 12.5/27 or <span style="color:red;">42.593% to 46.296%</span>
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− | :also reveals that a "R" gene was moved to the left splint
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− | | |
− | Left Splint:
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− | :Looking for where a junk G was replaced with a R gene, and no new genes were created. (Phenotype was not altered)
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− | Solvents on Left Splint showed:
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− | *ORGR
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− | *GYGY
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− | *YGYG
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− | *YORY
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− | *RYYR
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− | *ROYY
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− | *RGR
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− | *GYOR
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− | *RGYO
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− | *YYO
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− | *YGY
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− | *RGYG
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− | *YRR <--- confirms G --> R switch. Only two places it could be. 11th or 20th.
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− | *RRRO <-- proves 20th gene was swapped to R.
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− | | |
− | | |
− | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYR<span style="border:1px solid green;">R</span>(RO)YY(OR)RK
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− | :gene targeted was 20/27
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− | :gene range is 19.5/27 to 20.5/27 or <span style="color:red;">72.222% to 75.926%</span>
| |
− | </div>
| |
− | | |
− | | |
− | <div style="margin:30px">
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− | TEST 2: Silken / Clarity (Ava 28, Ava 29)
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− | :phenotypes: Silken bulb unchanged, Clarity had a cyan gene broken.
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− | | |
− | :Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK
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− | :left target 72.222%-75.962% is 13/18 gene --> 1st U.
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− | | |
− | :Right splint: Clarity Genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GORR)GRORGRROK
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− | :right target 42.593%-46.296% is 16/36 gene --> 2nd R in the first cyan gene set (GORR)
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− | :resultant right genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GOR<span style="border:1px solid green;">U</span>)GRORGRROK
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− | | |
− | :we know now what left genome is, since it's U was replaced with an R from Clarity:
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− | :resultant left genome: KRRROOOYYYGGG<span style="border:1px solid green;">R</span>UUIIIK
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− | | |
− | These results are compatible with observations
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− | Left Splint Solvent:
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− | GGGR. That confirms it.
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− | 13th gene targeted.
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− | range is 12.5/18 - 13.5/18 = 69.444% to 75.000%
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− | Combining with Test 1 results, new Left range is <span style="color:red;">72.222% to 75.000%</span>
| |
− | | |
− | | |
− | | |
− | </div>
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 3: Vampire/ Vampire (Bessodia's Test)
| |
− | Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K
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− | | |
− | Left Splint:
| |
− | *YOOI
| |
− | *IOIY
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− | *IYIO
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− | *IYOO
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− | *YIYI
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− | *IYIY
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− | *OOIO
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− | *OIYO
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− | *YIOI
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− | *IOIK
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− | *KIYI
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− | | |
− | 9th gene was swapped: : K(IY)(IY)(IO)(IY)<span style="border:1px solid green;">O</span>O(IO)K
| |
− | range is 8.5/12 to 9.5/12 = 70.833% to 79.167%.
| |
− | That is consistent w/ other tests.
| |
− | | |
− | Right Splint:
| |
− | *IYII
| |
− | *IIIY
| |
− | *IOIK
| |
− | *IIYI
| |
− | *KIYI
| |
− | *YIOI
| |
− | 6th gene was swapped: KIYIYI<span style="border:1px solid green;">I</span>IYIOIOK
| |
− | range is 45.833% to 54.167%.
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− | That narrows down the range!! now it is <span style="color:red;"> 45.833% to 46.296%.</span>
| |
− | | |
− | </div>
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 4: Crown / Fracture
| |
− | MEANT to do crown / clarity....oops
| |
− | | |
− | yielded Ava 121, Ava 122
| |
− | | |
− | Left: Broke UROR (Yellow West). Not sure if gene 27 (R) or gene 28(O) w/o using solvents.
| |
− | | |
− | Right: (ROYG)(ROY_YOR)GOR(GOO)
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− | broke that middle _ gene (was a G). it broke up a giant and a dwarf gene, leaving just 1 giant gene in the genome.
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− | can't tell what was swapped in, w/o solvent.
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− | | |
− | test inconclusive, w/o solvents.
| |
− | </div>
| |
− | | |
− | | |
− | <div style="margin:30px">
| |
− | TEST 5: Crown/Clarity (Ava 129. Ava 130)
| |
− | | |
− | expected: Crown's gene 27 or 28 ... UROR... (the RO in the middle)
| |
− | expected: Clarity's genes 15, 16, 17.
| |
− | gene 15, 16 are R's in GORR
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− | gene 17 is G in GROR
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− | | |
− | Observed: phenotypes of flowers are the same!!
| |
− | so both splints swapped R's.
| |
− | | |
− | Left: gene 27 of 37 was targeted
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− | range is 26.5/37 to 27.5/37 or 71.612% to 74.324%
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− | combining with other test results, the new left splint range is:<span style="color:red;"> 72.222% to 74.324%.</span>
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− | | |
− | Right Splint:
| |
− | was gene 15 or 16 of 36 total
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− | range is 14.5/36 to 16.5/36 or 40.278% to 45.833%
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− | <span style="color:red;"> This overlaps exactly at 45.833%.... range not updated though.</span>
| |
− | | |
− | ===Tay 04 ===
| |
− | Cracky, Prickly, Spongy Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
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− | | |
− | applied mutagen.
| |
− | Observed:
| |
− | :both new splints had same phenotypes. (Ava 49/Ava 50)
| |
− | | |
− | Both splints had junk genes swapped, or same type of gene swapped.
| |
− | | |
− | Solvents on Right Splint showed:
| |
− | *not tested yet
| |
− | | |
− | :Solvents on Left Splint showed:
| |
− | *KORG
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− | *ORGR
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− | *RYY
| |
− | *YGY, GYG
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− | *YORY
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− | *GROY
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− | *ORYY
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− | *GYGY
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− | *RGRO
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− | *YYRG
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− | *YRGR
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− | *GYGR
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− | *RGYO
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− | | |
− | no anomolies found, and standard junk genes all covered.
| |
− | Seems that the <span style="color:red;">Same Gene type</span> was swapped between splints :(
| |
− | further tests are needed to characterize this mutagen
| |
− | | |
− | </div>
| |
− | | |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: Vampire / vampire (Bessodia's Test)
| |
− | Vampire: KIYIYIOIYIOIOK 12 genes
| |
− | | |
− | Right splint:
| |
− | *IYIO
| |
− | *YYIO
| |
− | *YIOI
| |
− | *KIYY
| |
− | *IYYY
| |
− | *IOIY
| |
− | *OIYI
| |
− | *IYYY
| |
− | *0IOK
| |
− | 3rd gene was swapped: KIY<span style="border:1px solid green;">Y</span>YIOIYIOIOK
| |
− | range is 2.5/12 to 3.5/12 which is <span style="color:red;">20.833% to 29.167%</span>
| |
− | | |
− | Left Splint:
| |
− | *KIII
| |
− | 2nd gebe was swapped. KI<span style="border:1px solid green;">I</span>IYIOIYIOIOK
| |
− | range is 1.5/12 to 2.5/12 which is <span style="color:red;">12.500% to 20.833%</span>
| |
− | | |
− | </div>
| |
− | | |
− | ===Tay 05 ===
| |
− | Crackly, Dry, spongy Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen.
| |
− | Observed:
| |
− | :both new splints had same phenotypes. (Ava 39/Ava 40)
| |
− | | |
− | Both splints had junk genes swapped, or same type of gene swapped.
| |
− | | |
− | Solvents on Right Splint showed:
| |
− | *not tested yet
| |
− | | |
− | :Solvents on Left Splint showed:
| |
− | *GYGR
| |
− | *RYYR
| |
− | *YORR
| |
− | *GROY
| |
− | *OYY
| |
− | *YRG
| |
− | *RGR
| |
− | *GYOR
| |
− | *RGYO
| |
− | *GYG
| |
− | *OYYO
| |
− | *ROYY
| |
− | *GRGY
| |
− | | |
− | no anomolies found, and standard junk genes all covered.
| |
− | Seems that the <span style="color:red;">Same Gene type</span> was swapped between splints :(
| |
− | | |
− | </div>
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: Blush / Morning (Ava 31, 32)
| |
− | :phenotypes: Both phenotypes were unchanged
| |
− | | |
− | :Left splint: Blush Genome: KGRORVGOOOVUROOVIOVIOOVIOOOK
| |
− | :Right splint: Morning: KIRRRIYYYIYYYUYYYUYYYURORURORGRRRGYYYGORRGRROK
| |
− | :so far, no conclusions made
| |
− | </div>
| |
− | | |
− | | |
− | <div style="margin:30px">
| |
− | TEST 3: Vampire / vampire (Bessodia's Test)
| |
− | Vampire: KIYIYIOIYIOIOK 12 genes
| |
− | | |
− | Right splint:
| |
− | *IIOI
| |
− | *IIIO
| |
− | *IYIY
| |
− | *IYIO
| |
− | *IOIO
| |
− | *YIYI
| |
− | *IOII
| |
− | *KIYI
| |
− | *OIOK
| |
− | | |
− | 8th gene was swapped: KIYIYIOI<span style="border:1px solid green;">I</span>IOIOK
| |
− | range is 7.5/12 to 8.5/12 which is <span style="color:red;">62.500% to 70.833%</span>
| |
− | | |
− | Left Splint:
| |
− | *YIYI
| |
− | *IOYY
| |
− | *YIOI
| |
− | *OIOK
| |
− | *IOYY
| |
− | *KIYI
| |
− | *OYYI
| |
− | | |
− | 7th gene was swapped. KIYIYIO<span style="border:1px solid green;">Y</span>YIOIOK
| |
− | range is 6.5/12 to 7.5/12 which is <span style="color:red;">54.167% to 62.500%</span>
| |
− | | |
− | </div>
| |
− | | |
− | | |
− | | |
− | ===Tay 06===
| |
− | Fuzzy, Hairy, SPongy, Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 33/Ava 34)
| |
− | Observed:
| |
− | :Left Spoint: 1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | :Right Splint: 2 weed/0 water/1 seed / 1 flax/ 0 rotten
| |
− | | |
− | Right splint was affected in (GYGYGY) gene, specifically NOT the middle GY genes.
| |
− | Left splint had a junk gene swapped.
| |
− | | |
− | Solvents on Right Splint showed:
| |
− | *RYY
| |
− | *RGYG
| |
− | *GGRG <-- that pinpoints it as the third Y in (GYGYGY) being replaced with a G
| |
− | | |
− | Right Genome: K(OR)GR(GYGYG<span style="border:1px solid green;">G</span>)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | :gene targeted was 10/27.
| |
− | :gene range is 9.5/27 to 10.5/27 or <span style="color:red;">35.185% to 38.889%</span>
| |
− | :also reveals that a "Y" gene was moved to the left splint
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *KOR
| |
− | *ORRK
| |
− | *RGY
| |
− | *YYOR
| |
− | *ROOY
| |
− | *ORYY
| |
− | *GYY <--- proves it
| |
− | :Looking for where a junk G was replaced with a Y gene, and no new genes were created. (Phenotype was not altered)
| |
− | | |
− | Left Genome: K(OR)GR(GYGYGY)<span style="border:1px solid green;">Y</span>RGY(OR)YYRG(RO)YY(OR)RK
| |
− | :gene targeted was 11/27
| |
− | :gene range is 10.5/27 to 11.5/27 or <span style="color:red;">38.889% to 42.593%</span>
| |
− | </div>
| |
− | | |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: Ava 45 / Blush
| |
− | | |
− | This is Step #2 in section below, trying for 75% green flower.
| |
− | Left: (UYYY) yellow gene was broken on Ava 45.
| |
− | need to do solvent testing to know which gene it was exactly.
| |
− | | |
− | Right: predicted either gene 9 or 10 to swap.
| |
− | gene 9 would have broken (GOOO)
| |
− | Gene 0 is a V. no phenotype change expected.
| |
− | No phenotype change of blush was seen. Therefore, we know 10th gene V swapped.
| |
− | 10/26 gene targeted
| |
− | range is 9.5/26 to 10.5/26 = 36.538% to 40.385%
| |
− | combining with test 1, that gives overlap range of <span style="color:red;">36.538% to 38.889%.</span>
| |
− | </div>
| |
− | | |
− | | |
− | ===Tay 07===
| |
− | Crackly, Hairy, Prickly Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 51/Ava 52)
| |
− | Observed:
| |
− | :Left Spoint: 2 weed/1 water/1 seed/2 flax/0 rotten had an extra weed!
| |
− | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten not changed
| |
− | | |
− | Left splint was affected in (OR) gene.
| |
− | Right splint had a junk gene swapped.
| |
− | | |
− | Solvents on Left Splint (Ava51) showed:
| |
− | *YYOY <-- that pinpoints it as the last (OR) broken. R was replaced w/ Y.
| |
− | :Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(O<span style="border:1px solid green;">Y</span>))RK
| |
− | :gene targeted was 26/27
| |
− | :gene range is 25.5/27 to 26.5/27 or <span style="color:red;">94.444% to 98.145%</span>
| |
− | | |
− | Right splint had a junk Y replaced.
| |
− | Solvents on Right Splint showed:
| |
− | *GRGY
| |
− | *GROY
| |
− | *GYGY
| |
− | *RGRO
| |
− | *RGRG
| |
− | *RRK
| |
− | *YRG
| |
− | *YYO
| |
− | *YOR
| |
− | *ORG
| |
− | *ORR <--- pinpoints it (after other solvents eliminated some possibly Y's).
| |
− | The 17th Y gene is swapped out and was replaced with an R
| |
− | :Right genome: K(OR)GR(GYGYGY)GRGY(OR)<span style="border:1px solid green;">R</span>YRG(RO)YY(OR))RK
| |
− | :gene targeted was 17/27
| |
− | :gene range is 16.5/27 to 17.5/27 or <span style="color:red;">61.111% to 64.815%</span>
| |
− | </div>
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: Clarity / Morning
| |
− | (Ava 69, Ava 70)
| |
− | | |
− | Left: this will swap either the 34th or 35th gene in Clarity. Both are R genes.
| |
− | :.... GRROK.
| |
− | | |
− | Right: this will swap either the 27, 28 or 29 gene (of 44) in Morning.
| |
− | :original genome: ...(UROR)(GRRR)...
| |
− | if 27th, (UROR) --> (URRR). This will remove Yellow Inner West and add Cyan Inner East.
| |
− | if 28th (UROR) ---> (UROR). No change in genome or phenotype
| |
− | if 29th (GRRR)--> RRRR. this removes cyan from the outer south, and adds nothing.
| |
− | | |
− | Observed: Yellow inner west was removed and cyan inner east was added.
| |
− | Right genome:KIRRRIYYYIYYYUYYYUYYY(UROR)(UR(O<span style="border:1px solid green;">R</span>)R)GRRRGYYYGORRGRROK
| |
− | :gene targeted was 27/44.
| |
− | :gene range is: 26.5/44 to 27.5/44 or 60.227% to 62.500%.
| |
− | :combined with TEST 1's results, Right splint range is now: <span style="color:red;">61.111% to 62.500%</span>
| |
− | | |
− | Left: GRRO gene was indeed broken, removing a yellow and leaving, in total, 1 black and 1 blue color on the Outer North Petals.
| |
− | No further conclusions can be made about which R gene was swapped.
| |
− | </div>
| |
− | | |
− | | |
− | <div style="margin:30px">
| |
− | TEST 3: Vampire/vampire (Bessodia's Test)
| |
− | Left genome: IYIYIOIOIOIO.
| |
− | 8th gene 0-->Y
| |
− | range is 62.500% to 70.833%
| |
− | <span style="color:red;">overlaps only at edge of range, 62.500%</span>
| |
− | | |
− | Right genome: IYIYIOIYIOIY
| |
− | 12th gene Y-->O
| |
− | range is 95.833% - 100%
| |
− | updates range to <span style="color:red;">95.833% to 98.143%</span>
| |
− | | |
− | ===Tay 08 ===
| |
− | Crackly, Phosphorescent, Slimy Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 53/Ava 54)
| |
− | Observed:
| |
− | :Left Spoint: 0 weed/1 water/1 seed/0 flax/0 rotten ... no weed and no flax are different
| |
− | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step
| |
− | | |
− | Left splint was affected in ROY sequence (which is required to harvest flax, see flax genome theories for more info). However, it was not a *(RO) that was changed, or the seed count would have gone down. Furthermore, we need a new OR gene, to account for the 1 less weed step.
| |
− | :looks like the 2nd to last Y should be switched to a R. ... YYRG(RO)RY(OR)RK. This would add (OR) and removed the (ROY).
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *GYG
| |
− | *RGR
| |
− | *ROR <-- that pinpoints it as the gene we suspected.
| |
− | :the 22nd gene, Y, is replaced with an R
| |
− | :gene range is 21.5/27 to 22.5/27 or <span style="color:red;">83.333% to 87.037%</span>
| |
− | :Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)<span style="border:1px solid green;">R</span>Y(OR)RK
| |
− | | |
− | Right splint lost an (-1 weed) gene, so (OR) was broken somewhere in the genome.
| |
− | We know a R was replaced with a Y, from the left splint tests.
| |
− | | |
− | Solvents on Right Splint showed:
| |
− | *ORY
| |
− | *ROY
| |
− | *GRGY
| |
− | *GYGY
| |
− | *YOR
| |
− | *ROYY
| |
− | *YYRG
| |
− | *RGR
| |
− | *OYYO
| |
− | *YORY
| |
− | *KOY <--- shows its the second gene, R, that is swapped with a Y from the other splint
| |
− | :gene targeted was 2/27.
| |
− | :gene range is 1.5/27 to 2.5/27 or <span style="color:red;">5.556% to 9.259%</span>
| |
− | :Right Genome: K(O<span style="border:1px solid green;">Y</span>)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | </div>
| |
− | | |
− | ===Tay 09 ===
| |
− | Calico, Green, Smelly Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen.
| |
− | Observed:
| |
− | :both new splints had same phenotypes. (Ava 55/Ava 56)
| |
− | | |
− | Both splints had junk genes swapped, or same type of gene swapped.
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *GRG
| |
− | *GRO
| |
− | *GYGY
| |
− | *YGYG
| |
− | *ORYY
| |
− | *ORGR
| |
− | *YORY
| |
− | *YYOR
| |
− | *RGYO
| |
− | *OYYO
| |
− | *GYGR
| |
− | *RYY
| |
− | *YRGR
| |
− | *ORRK
| |
− | no anomolies found yet. Standard Junk genes all covered.
| |
− | | |
− | This mutation swapped same gene type between splints
| |
− | :this test is inconclusive
| |
− | </div>
| |
− | | |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: Vampire/Vampire (Bessodia's Test)
| |
− | | |
− | R: KIYOYIOIYIOIOK
| |
− | 3rd gene was switched
| |
− | range is 2.5/12 to 3.5/12 or <span style="color:red;">20.833% to 29.157%</span>
| |
− | | |
− | Left: KIYIYIIIYIOIOK
| |
− | 6th gene was switched
| |
− | range is 5.5/12 to 6.5/12 or <span style="color:red;">45.833% to 54.167%</span>
| |
− | | |
− | </div>
| |
− | | |
− | ===Tay 10===
| |
− | Crackly, Spotted, Striped , Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 57/Ava 58)
| |
− | Observed:
| |
− | :Left Spoint: 2 weed/0 water/1 seed/0 flax/0 rotten
| |
− | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten
| |
− | | |
− | Left splint was affected in (GYGYGY) gene, specifically in the middle GY genes.
| |
− | This breaks up both Flax genes (GYGY) as well as the water gene (GYGYGY), which would account for our observations.
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *YGRG <-- that pinpoints it as the middle Y in (GYGYGY) being replaced with a R
| |
− | :Left Genome: K(OR)GR(GYG<span style="border:1px solid green;">R</span>GY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | :gene targeted was 8/27.
| |
− | :gene range is 7.5/26 to 8.5/27 or <span style="color:red;">27.778% to 31.481%</span>
| |
− | | |
− | Right splint had a -1 weed gene broken (OR). From above, we know it was the R that was swapped with a Y from the Left splint.
| |
− | Just have to identify which of the three (OR) genes was broken.
| |
− | Solvents on Right Splint showed:
| |
− | *RGRG
| |
− | *RGY
| |
− | *GYG
| |
− | *YRGY
| |
− | *RGY
| |
− | *ORG
| |
− | *YGY
| |
− | *ORY
| |
− | *RGY
| |
− | *YOR
| |
− | *YYR
| |
− | *RRY
| |
− | *GYO
| |
− | *OYRB <--- pinpoints the (OR) gene at the end as switching to (OY).
| |
− | | |
− | Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(O<span style="border:1px solid green;">Y</span>)RK
| |
− | :gene targeted was 26/27
| |
− | :gene range is 25.5/27 to 26.5/27 or <span style="color:red;">94.444% to 98.148%</span>
| |
− | </div>
| |
− | | |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: morning / Ava 28
| |
− | yields flowers Ava 103, Ava 104
| |
− | | |
− | Left:
| |
− | Above test 1 predicts gene 12-14 will be chosen, and that Ava 28 will donate a "I" gene
| |
− | morning genome: K IRRR IYYY IYYY UYYY ...
| |
− | if 12th gives ... IYYI UYYY... would lose 1 yellow on inner stamen
| |
− | if 13th, gives ... IYYY IYYY... would lose 1 yellow East, gain 1 yellow on entire stamen
| |
− | if 14th, gives ... IYYY UIYY... would lose 1 yellow East, gain 1 yellow on outer and middle stamen
| |
− | | |
− | Observed: top and middle staymen are same. Inner stamen is more blueish. East petals are the same.
| |
− | therefore, the 12th gene was exchanged.
| |
− | so the range is 11.5/44 to 12.5/44 or 26.778% to 28.409%
| |
− | combining that with Test 1 results, the new range is <span style="color:red;">27.778% to 28.409%</span>
| |
− | | |
− | Right:
| |
− | Test 1 predicted that gene 17 or 18 would be switched.
| |
− | Ava 28 genome is: KRRROOOYYYGGGRUUIIIK
| |
− | Either is an I gene.
| |
− | if 17th, gives ...UUIYIK would introduce yellow on the top stamen
| |
− | if 18th, gives ...UUIIYK would still appear all white.
| |
− | | |
− | Observed: the top stamen has 1 shade of yellow now.
| |
− | therefore, the 17th gene was swapped.
| |
− | the range is 16.5/18 to 17.5/18 or 91.667% to 97.222%
| |
− | combining hat with Test 1 results, the new range is <span style="color:red;">94.444% to 97.222%</span>
| |
− | </div>
| |
− | | |
− | TEST 3: Vampire/Vampire (Bessodia's Test)
| |
− | | |
− | Left: KIYIOIOYIOIOK
| |
− | 4th gene swapped
| |
− | range is 3.5/12 to 4.5/12 or 29.167% to 37.500%
| |
− | Doesn't quite match tests above...
| |
− | | |
− | Right: KIYIYIOIYIOIK
| |
− | 12th gene is switched
| |
− | range is 95.833% to 100%
| |
− | combining with above test results...
| |
− | New range is <span style="color:red;">95.833% to 97.222%</span>
| |
− | </div>
| |
− | | |
− | ===Tay 11 ===
| |
− | Phosphoresecent, Slimy, Smelly Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 59/Ava 60)
| |
− | Observed:
| |
− | :Left Spoint: same phenotype
| |
− | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step
| |
− | | |
− | Right splint had an extra weeding step. This means an (OR) gene was broken up.
| |
− | | |
− | Solvents on Right Splint showed:
| |
− | *YRRR <-- that pinpoints it as the last O gene --> R gene.
| |
− | :the 25nd gene, O, is replaced with an R
| |
− | :gene range is 24.5/27 to 25.5/27 or <span style="color:red;">90.741% to 94.444%</span>
| |
− | :Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(<span style="border:1px solid green;">R</span>R)RK
| |
− | | |
− | Left splint lost an R gene in the junk genes somewhere, which was replaced with an O gene.
| |
− | Solvents on Left Splint showed:
| |
− | *YGRG
| |
− | *OGRO <--- shows its the 19th gene, R, that is swapped with a G from the other splint.
| |
− | :gene targeted was 19/27.
| |
− | :gene range is 18.5/27 to 19.5/27 or <span style="color:red;">68.519% to 72.222%</span>
| |
− | :Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YY<span style="border:1px solid green;">O</span>G(RO)YY(OR)RK
| |
− | </div>
| |
− | | |
− | | |
− | ===Tay 12 ===
| |
− | Calico, Crackly, Prickly Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen.
| |
− | Observed:
| |
− | :both new splints had same phenotypes. (Ava 61/Ava 62)
| |
− | | |
− | Both splints had junk genes swapped, or same type of gene swapped.
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *ORYY
| |
− | *GYGR
| |
− | *ROYY
| |
− | *ORGR
| |
− | *YRGR
| |
− | *YYOR
| |
− | *RGYG
| |
− | *RGRG
| |
− | *GYGY
| |
− | *GRGY
| |
− | *GYO
| |
− | *RGRO
| |
− | *RRK
| |
− | no anomolies found yet. Standard Junk genes all covered.
| |
− | | |
− | This mutation swapped same gene type between these splints.
| |
− | :This test is inconclusive
| |
− | </div>
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: Vampire/Vampire (Bessodia's Test)
| |
− | | |
− | Left: KIOIYIOIYIOIOK
| |
− | 2nd gene switched
| |
− | range is 1.5/12 to 2.5/12 or <span style="color:red;">13.500% to 20.833%</span>
| |
− | | |
− | Right: KIYIYIYIYIOIOK
| |
− | 6th gene switched
| |
− | range is 5.5/12 to 6.5/12 or <span style="color:red;">45.833% to 54.167%</span>
| |
− | | |
− | </div>
| |
− | | |
− | ===Tay 13===
| |
− | Calico, Slimy, Smelly Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 63/Ava 64)
| |
− | Observed:
| |
− | :Left Spoint: no seeds!
| |
− | :Right Splint: no change in phenotype
| |
− | | |
− | Left splint was affected in (RO) gene, since it gave 0 seeds.
| |
− | Left splint had a junk gene swapped, to either a R or an O.
| |
− | | |
− | Solvents on Right Splint showed:
| |
− | *YORR
| |
− | *YGYG
| |
− | *ORYY
| |
− | *ROYY
| |
− | *YORY
| |
− | *YYOG <-- that pinpoints it as 3rd Junk R gene, being replaced with an O.
| |
− | | |
− | Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YY<span style="border:1px solid green;">O</span>G(RO)YY(OR)RK
| |
− | :gene targeted was 19/27.
| |
− | :gene range is 18.5/27 to 19.5/27 or <span style="color:red;">68.519% to 72.222%</span>
| |
− | | |
− | :The above pinpoints that the O gene in the (RO) gene set was replaced with an R.
| |
− | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRGR<span style="border:1px solid green;">R</span>YY(OR)RK
| |
− | :gene targeted was 22/27
| |
− | :gene range is 21.5/27 to 22.5/27 or <span style="color:red;">79.630% to 83.333%</span>
| |
− | </div>
| |
− | | |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: Vampire/Vampire (Bessodia's Test)
| |
− | | |
− | Left: KIYIYIOIYIIIOK
| |
− | 10th gene switched
| |
− | range is 9.5/12 yo 10.5/12 or 79.167% to 87.500%
| |
− | | |
− | Right: KIYIYIOIYOOIOK
| |
− | 9th gene switched
| |
− | range is 8.5/12 to 9.5/12 or 70.833% to 79.167%
| |
− | combining w/ Test 1 results narrows the range to <span style="color:red;">70.833% to 72.222%</span>
| |
− | </div>
| |
− | | |
− | ===Tay 14===
| |
− | Fuzzy, Reticulated, Spotted, Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 65/Ava 66)
| |
− | Observed:
| |
− | :Left Spoint: 1 extra weed
| |
− | :Right Splint: 1 extra seed
| |
− | | |
− | This means a new (RO) gene was created in the right gene, while a (OR) gene was broken in the left gene.
| |
− | | |
− | Solvents on Left Splint (Ava 65) showed:
| |
− | *ROR (narrows it down to one of the end Y genes. Specifically, RGR could not become ROR, or there would be a new (OR) gene made as well, and it would be 0 weeds.)
| |
− | *YGR
| |
− | *YYR
| |
− | *YOR
| |
− | *KOR
| |
− | *GRO
| |
− | *YROR <-- that pinpoints it as the last Y in the genome being replaced with a R.
| |
− | :gene targeted was 24/27.
| |
− | :gene range is 23.5/27 to 24.5/27 or <span style="color:red;">87.037% to 90.741%</span>
| |
− | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)Y<span style="border:1px solid green;">R</span>(OR)RK
| |
− | | |
− | Right Splint will have an R in (OR) gene replaced with a Y gene.
| |
− | Solvents on Right Splint showed:
| |
− | *RGY
| |
− | *YORY
| |
− | *YYOY <--- proves it to be the 2nd last R in genome.
| |
− | :gene targeted is 25/27.
| |
− | :gene range is 24.5/27 to 25.5/27 or <span style="color:red;">90.741% to 94.444%</span>
| |
− | Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YYO<span style="border:1px solid green;">Y</span>RK
| |
− | | |
− | </div>
| |
− | | |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: Vampire/Vampire (Bessodia's Test)
| |
− | | |
− | Left: KIYIYIOIYIOIIK
| |
− | 12th gene switched
| |
− | range is 11.5/12 to 12/12 or 95.833% to 100%
| |
− | | |
− | R: KIYIYIOIYIOOOK
| |
− | 11th gene is switched
| |
− | range is 10.5/12 to 11.5/12 or 87.500% to95.833%
| |
− | | |
− | *I suspect these splints were accidentally switched...
| |
− | | |
− | </div>
| |
− | | |
− | ===Tay 15===
| |
− | Fuzzy Green Mottled Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 86/Ava 87)
| |
− | Observed:
| |
− | :Left Spoint: no water and no flax!
| |
− | :Right Splint: 1 water, 2 weeds. (one extra weed step)
| |
− | | |
− | Left splint had (GYGYGY) gene groups broken in the middle GY
| |
− | Left splint had an(OR) gene group broken.
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *ORGR
| |
− | *KORG
| |
− | *GYRY <-- that pinpoints it as (GYGYGY) --> (GYRYGY)
| |
− | Left Genome: K(OR)GR(GY<span style="border:1px solid green;">R</span>YGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | :gene targeted was 7/27.
| |
− | :gene range is 6.5/27 to 7.5/27 or <span style="color:red;">24.074% to 27.777%</span>
| |
− | :also reveals that a "G" gene was moved to the right splint
| |
− | | |
− | :Right Splint:
| |
− | :The phenotype revealed that the (OR) -1 weed gene was mutated.
| |
− | :The right gene results show that an "R" gene was switched w/ a "G" gene. So we are looking for (OR) -->OG
| |
− | Solvents on Right showed:
| |
− | *YYRG
| |
− | *GROY
| |
− | *RGRO
| |
− | *GYOR
| |
− | *OGGR <-- pinpoints as the first (OR) changed.
| |
− | Right Genome: KO<span style="border:1px solid green;">G</span>GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | :gene targeted was 2/27
| |
− | :gene range is 1.5/27 to 2.5/27 or <span style="color:red;">5.556% to 9.259%</span>
| |
− | </div>
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 2: Vampire/Vampire (Bessodia's Test)
| |
− | | |
− | Left: KIYIIIOIYIOIOK
| |
− | 4th gene switched
| |
− | range is 3.5/12 to 4.5/12 or 29.167% to 37.500% ... a bit out of range w/ Test 1 results
| |
− | | |
− | Right: KYYIYIOIYIOIOK
| |
− | 1st gene switched
| |
− | range is 0/12 to 1.5/12 or 0% to 12.5%
| |
− | | |
− | </div>
| |
− | | |
− | ==Sterope Recipes==
| |
− | | |
− | ===Sterope 03===
| |
− | Dry, Fuzzy, Simey Aryballa opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 67/Ava 68)
| |
− | Observed:
| |
− | :Left Spoint: 0 weed!! ... got a new (OR) gene
| |
− | :Right Splint: 2 weed !! ... broke a (OR) gene
| |
− | | |
− | Solvents on Left Splint (Ava 67) showed:
| |
− | *ORR
| |
− | *YGYG
| |
− | *GYGY
| |
− | *GYGR
| |
− | *ORGR
| |
− | *GRGY
| |
− | *YORR
| |
− | *ORYO <-- that pinpoints it as the 6th Y being replaced with an O.
| |
− | | |
− | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)Y<span style="border:1px solid green;">O</span>RG(RO)YY(OR)RK
| |
− | :gene targeted was 18/27
| |
− | :gene range is 17.5/27 to 18.5/27 or <span style="color:red;">64.815% to 68.519%</span>
| |
− | | |
− | Solvents on Right Splint (Ava 68) showed:
| |
− | *ORR
| |
− | *KYR <--- proves it. First O gene is replaced with a Y.
| |
− | | |
− | Right Genome: K<span style="border:1px solid green;">Y</span>RGR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | :gene targeted is 1/27
| |
− | :gene range is 0.5/27 to 1.5/27 or <span style="color:red;">1.852% to 5.556%</span>
| |
− | | |
− | </div>
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Dusk/Ava 26
| |
− | should swap the 1st R from Ava 26. (if not, next Gene is an R too)
| |
− | | |
− | for Left splint, 4 possibilities:
| |
− | | |
− | 47th gene: GROR --> GRRR north magenta broken, South Cyan gained
| |
− | 48th gene: GROR --> GROR no changes, same gene swapped (R)
| |
− | 49th gene: IOOO --> ROOO broke magenta on outer, inner and middle stamen
| |
− | 50th gene: IOOO --> IROO broken magenta on outer, inner and middle stament, gained cyan on outer stamen
| |
− | | |
− | Observed:
| |
− | Left splint (Ava 99) looks exactly like Dusk
| |
− | Right splint (Ava 100) is all white.
| |
− | | |
− | Therefore, the 48th gene was swapped from Left splint.
| |
− | 48/73 gene
| |
− | range is now 47.5/73 to 48.5/73 or <span style="color:red;">65.068% to 66.438%</span>
| |
− | | |
− | | |
− | | |
− | </div>
| |
− | | |
− | ===Sterope 04===
| |
− | Crackly, Dry Reticulated Sabahl opened
| |
− | | |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen.
| |
− | Observed:
| |
− | :both new splints had same phenotypes. (Ava 88/Ava 89)
| |
− | | |
− | Both splints had junk genes swapped, or same type of gene swapped.
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *ORYY
| |
− | *GRGY
| |
− | *YORY
| |
− | *ORRK
| |
− | *RGYO
| |
− | *OYYO
| |
− | *RGRO
| |
− | *RYYR
| |
− | *RGYG
| |
− | *GROY
| |
− | no anomolies found yet. Standard Junk genes 4/27 and 11/27 not covered conclusively.
| |
− | | |
− | :Solvents on Right Splint showed:
| |
− | *RGYG
| |
− | *ORRK
| |
− | *RGYO
| |
− | *YRGR
| |
− | | |
− | | |
− | no anomolies found yet. Starting to think this mutagen swapped same genes.
| |
− | :this test is inconclusive (could go back to solvents more)
| |
− | </div>
| |
− | | |
− | ===Sterope 05===
| |
− | Phosphorescent, Prickly, Slimy Sabahl opened
| |
− | | |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen.
| |
− | Observed:
| |
− | :both new splints had same phenotypes. (Ava 118/Ava 120)
| |
− | | |
− | Both splints had junk genes swapped, or same type of gene swapped.
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *YYOR
| |
− | *YGRG
| |
− | *RGYG
| |
− | *GYOR
| |
− | *YORR
| |
− | *YORY
| |
− | *RGYO
| |
− | *KORG
| |
− | *RGRG
| |
− | *RGRO
| |
− | *ORGR
| |
− | *GRGY
| |
− | *YGYG
| |
− | *YYOR
| |
− | *OYYO
| |
− | no anomolies found yet. Standard Junk genes 18/27 not covered conclusively.
| |
− | Seems to be that the same genes were switched.
| |
− | </div>
| |
− | | |
− | ===Sterope 06===
| |
− | Calico, Crackly, Spongy Avanya opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 117, Ava 118)
| |
− | Observed:
| |
− | :Left Spoint: 1 more weeding
| |
− | :Right Splint: 2 seeds
| |
− | | |
− | Left splint had lost an (OR) gene
| |
− | Right splint had gained (RO) gene
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *GYGY
| |
− | *RGYY <-- that pinpoints it as the second (OR) --> YR
| |
− | | |
− | Left Genome: K(OR)GR(GYGYGY)GRGY<span style="border:1px solid green;">Y</span>RYYRG(RO)YY(OR)RK
| |
− | :gene targeted was 15/27.
| |
− | :gene range is 14.5/27 to 14.5/27 or <span style="color:red;">53.704% to 57.407%</span>
| |
− | :also reveals that a "O" gene was moved to the left splint
| |
− | | |
− | Solvents on Right Splint:
| |
− | *ORGR
| |
− | *YORR
| |
− | *RGYO
| |
− | *GRGY
| |
− | *ROYR --> points to the 17th gene Y --> O.
| |
− | | |
− | Right sGenome: K(OR)GR(GYGYGY)GRGY(OR)<span style="border:1px solid green;">O</span>YRG(RO)YY(OR)RK
| |
− | :gene targeted was 17/27
| |
− | :gene range is 16.5/27 to 17.5/27 or <span style="color:red;">61.111% to 64.815%</span>
| |
− | </div>
| |
− | | |
− | ==Celano Recipes==
| |
− | | |
− | ===Celano 01===
| |
− | Dry, Spongy, Spotted Renard opened
| |
− | | |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen.
| |
− | Observed:
| |
− | :both new splints had same phenotypes. (Ava 90/Ava 91)
| |
− | | |
− | Both splints had junk genes swapped, or same type of gene swapped.
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *RGYO
| |
− | *GYGR
| |
− | *YYRG
| |
− | *GYOR
| |
− | *KORG
| |
− | *YORY
| |
− | *YGYG
| |
− | *GYGY
| |
− | *ORGR
| |
− | *GROY
| |
− | *RYYR
| |
− | *RORG
| |
− | *YGRG
| |
− | | |
− | covered all of the genome except the last 4 genes. Not showing anything yet, so stopping to save solvents...
| |
− | no conclusions drawn yet
| |
− | | |
− | | |
− | ===Celano 02===
| |
− | Green, Mottled, Prickly Renard opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 92/Ava 93)
| |
− | Observed:
| |
− | :Left Spoint: normal
| |
− | :Right Splint: 2 weed, 1 flax 1 water.
| |
− | | |
− | Left splint had no gene groups broken
| |
− | Right splint had (GYGYGY) broken (but not middle GY)
| |
− | | |
− | Solvents on Right Splint showed:
| |
− | *RYYR
| |
− | *YYYG <-- that pinpoints it as the third G directly in(GYGYGY) as being replaced with an Y
| |
− | | |
− | Right Genome: K(OR)GR(GYGY<span style="border:1px solid green;">Y</span>Y)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | :gene targeted was 9/27.
| |
− | :gene range is 9.5/27 to 10.5/27 or <span style="color:red;">31.481% to 35.185%</span>
| |
− | :also reveals that a "G" gene was moved to the left splint
| |
− | | |
− | Solvents on Left Splint:
| |
− | *GYGY
| |
− | *GYGR
| |
− | *RGYR --> points to the 17th gene Y-->G.
| |
− | | |
− | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)<span style="border:1px solid green;">G</span>YRG(RO)YY(OR)RK
| |
− | :gene targeted was 17/27
| |
− | :gene range is 16.5/27 to 17.5/27 or <span style="color:red;">61.111% to 64.815%</span>
| |
− | </div>
| |
− | | |
− | ==Alcyone Recipes==
| |
− | | |
− | ===Alcyone 01===
| |
− | Calico, Crackly, Pricky
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
− | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
− | | |
− | applied mutagen. (Ava 105/Ava 106)
| |
− | Observed:
| |
− | :Left Spoint: had an extra weed step (OR) was broken
| |
− | :Right Splint: no change, must have mutated junk
| |
− | | |
− | Solvents on Left Splint showed:
| |
− | *GYGY
| |
− | *GYGR
| |
− | *GRGY
| |
− | *YRGR
| |
− | *OGYY <-- that pinpoints it as the 4th R being replaced with a G.
| |
− | | |
− | Left Genome: K(OR)GR(GYGYGY)GRGY(O<span style="border:1px solid green;">R</span>)YYRG(RO)YY(OR)RK
| |
− | :gene targeted was 16/27.
| |
− | :gene range is 15.5/27 to 16.5/27 or <span style="color:red;">57.407% to 61.111%</span>
| |
− | :also reveals that a "G" gene was moved to the left splint
| |
− | | |
− | :Righgt Splint:
| |
− | :The right gene results show that an "G" gene was switched w/ a "R" gene, in the junk sections
| |
− | Solvents showed:
| |
− | *KORG
| |
− | *YORY
| |
− | *RGRG
| |
− | *YGYG
| |
− | *YYRG
| |
− | *ORYY
| |
− | *YORR
| |
− | *GRGY
| |
− | *RYYR
| |
− | *YYOR
| |
− | *ORRK
| |
− | *YGRR <--- pinpoints as 6th G being replaced w/ an R.
| |
− | | |
− | Right Genome: K(OR)GR(GYGYGY)GR<span style="border:1px solid green;">R</span>Y(OR)YYRG(RO)YY(OR)RK
| |
− | :gene targeted was 13/27
| |
− | :gene range is 12.5/27 to 13.5/27 or <span style="color:red;">46.296% to 50.000%</span>
| |
− | </div>
| |
− | | |
− | | |
− | ===AC1 02 ===
| |
− | Green, Phosphorescent, Smelly Avanya opened
| |
− | | |
− | <div style="margin:30px">
| |
− | TEST 1: Nile Green / Nile Green
| |
− | Unmutated Genome:
| |
− | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
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− | ::1 weed/1 water/1 seed/2 flax/0 rotten
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− | | |
− | applied mutagen.
| |
− | Observed:
| |
− | :both new splints had same phenotypes. (Ava 131/Ava 132)
| |
− | | |
− | Both splints had junk genes swapped, or same type of gene swapped.
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− | | |
− | Solvents on Right Splint showed:
| |
− | *YYOR
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− | *RYYR
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− | *GYGY
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− | *YGRG
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− | *GRGY
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− | *YORR
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− | no anomolies found yet. Standard Junk genes mostly covered
| |
− | | |
− | :this test is inconclusive. Could do further solvent testing, but at this point, no results.
| |
− | </div>
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− | | |
− | ==Crosses for Lily Requirements==
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− | | |
− | ===Aiming for 75% Green petals ===
| |
− | :Stragety: Add cyan to one set of inner petals on Morning, then break one of the two yellow genes for that same set. This will turn that inner set of petals green. The outer two sets of petals are already green, so that will be 3/4 green.
| |
− | | |
− | <div style="margin:30px">
| |
− | CROSS 1: Morning / Crown with calico,dry, spotted (Sterope 01)
| |
− | Aiming to swap first genes of both splints.
| |
− | Unmutated Morning:
| |
− | : K(IRRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
− | : Cyan1OSMSIS(IRRR)+Yel2OSMSIS(IYYY)+Yel2IEP(UYYY)+Yel2IWP(UROR)+Cyan1OSP(GRRR)+Yel1OSP(GYYY)+Cyan1ONP(GORR)+Yel1ONP(GRRO)
| |
− | Unmutated Crown:
| |
− | : K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K
| |
− | : Red3=[Mag3IWP(UROO)+Ye3IWP(UROR)] + 5V
| |
− | | |
− | :This mutagen will Turn (IRRR) (cyan on stamen) into (URRR), cyan on Inner East Petal.
| |
− | | |
− | Applied mutagen. (Ava 45/Ava46)
| |
− | Observed:
| |
− | :Left Spoint(Ava 45): Inner east petals now greenish yellow. <span style="color:red;">the cyan add was a success!!</span>
| |
− | :Genome of Ava 45: K(<span style="border:1px solid green;">U</span>RRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
− | | |
− | :Right Splint(Ava 46): Inner petals look orange, not red
| |
− | :Genome of Ava 46: K<span style="border:1px solid green;">I</span>ROO(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K
| |
− | Right splint had first gene broken, UROO (magenta inner petals) --> IROO upper stamen cyan 1.
| |
− | inner petal set now 3 yellow shades + 2 magenta shades = red + red + yellow, or a dark orange
| |
− | </div>
| |
− | | |
− | <div style="margin:30px">
| |
− | CROSS 2: Ava45 / Blush with Fuzzy, Hairy, Spongy (Tay 06)
| |
− | : aiming to break up a (UYYY) inner yellow gene on Ava 45
| |
− | Mutation should swap gene 18/44 (Y) on Ava 45 with gene 10/26 (V) blush.
| |
− | Unmutated Ava 45: K(URRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
− | Unmutated Blush: K(GROR)V(GOOO)V(UROO)V(IO)V(IOO)V(IOOO)K
| |
− | | |
− | Ava 47 / 48 resulted.
| |
− | Observed:
| |
− | left splint (Ava47) does have predicted colors. Green on outside petal sets. one green and one yellow inner petal sets.
| |
− | right splint (Ava48): colors appear to be blush. No observed change.
| |
− | | |
− | Hypothesized Ava 47 genome:
| |
− | :K(URRR)(IYYY)(IYYY)(UYYY)UY<span style="border:1px solid green;">V</span>Y(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
− | Hypothesized Ava 48 Genome:
| |
− | : K(GROR)V(GOOO)<span style="border:1px solid green;">Y</span>(UROO)V(IO)V(IOO)V(IOOO)K
| |
− | | |
− | both are consistent with expected and observed results
| |
− | </div>
| |