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| | ''' | | ''' |
| | | | |
| − | Originally from the Bastet server, I transfered to Tale 5 only a few months ago with some friends. (Now we form the Bastet Hounds Guild). | + | Originally from the Bastet server, I transfered to Tale 5 with some friends. (Now we form the Bastet Hounds Guild). |
| | My primary passion in Egypt is glassblowing and glassmaking. I also enjoy the Test of Pyrotechnics, Thought puzzles, and crossbreeding flowers. | | My primary passion in Egypt is glassblowing and glassmaking. I also enjoy the Test of Pyrotechnics, Thought puzzles, and crossbreeding flowers. |
| | I am always open to helping people in any way that I can, so please chat me anytime. | | I am always open to helping people in any way that I can, so please chat me anytime. |
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| | This includes: | | This includes: |
| | *Mushrooms (Sand Spore, Slaves Bread, Razor's Edge, Peasant Foot, and Salt Water Fungus). | | *Mushrooms (Sand Spore, Slaves Bread, Razor's Edge, Peasant Foot, and Salt Water Fungus). |
| − | *Cobra Skins (as many as possible)
| |
| | | | |
| | I am also always happy to purchase | | I am also always happy to purchase |
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| | I am hoping to help Egypt make better use of its Portable Star Labs. I see so many of them laying around unused, or used as decorations. They are meant to be used and returned to a university of Art (preferably Sterope). I have a Law Petition circulating to help us use them more effectively. Please remember they are used for opening new Star recipes only...they are NOT part of the Principle or Test of Pyrotechnics!! They are given out in a limited amount, so they are a precious resource. Please see http://www.atitd.org/wiki/tale5/Portable_Star_Lab if you have any questions, or chat me in game. | | I am hoping to help Egypt make better use of its Portable Star Labs. I see so many of them laying around unused, or used as decorations. They are meant to be used and returned to a university of Art (preferably Sterope). I have a Law Petition circulating to help us use them more effectively. Please remember they are used for opening new Star recipes only...they are NOT part of the Principle or Test of Pyrotechnics!! They are given out in a limited amount, so they are a precious resource. Please see http://www.atitd.org/wiki/tale5/Portable_Star_Lab if you have any questions, or chat me in game. |
| | | | |
| − | ==Mutagenics Research:==
| + | In addition, I am happy to help anyone with passing the principle of Pyrotechnics. If you have questions how to use a mortar, make stars, or anything pyro related, please chat me. If I can't help, I can probably direct you to someone that can! |
| − | I am trying to help characterize the mutagens that are being researched. | |
| − | Specially, I'm trying to determine which gene ranges are swapped from the left and right splints.
| |
| − | I won't bore you here with genetics theory, it is all explained much more clearly elsewhere on the wiki. | |
| − | But I would like to make my data available to others that share this interest.
| |
| | | | |
| − | This is my first time doing this, so PLEASE chat me if you think I am doing something wrong. Right now, our guru's of genetics are not playing, so I don't have a good source to verify this with. I welcome all input!!
| + | ==Mutagenics Research== |
| | + | You can find my test data for mutagens at the following page: |
| | + | http://www.atitd.org/wiki/tale5/User:Avanya/mutagenics |
| | + | My most recent data is not on this page, because it got too long to put it all on there, lol. But if you are interested, please chat me, and I can get you copies of data from my notes. |
| | + | ==Test Ideas== |
| | + | [[Test of Gemology]] |
| | | | |
| − | :'''I am not sure how the mutagen range rounds'''.
| + | [[Test of Sponsorship]] |
| − | For example, if a mutagen range is that it will swap a gene at 10%. Given a genome of length 16, 10% along is between gene 1 and 2. Does it pick the closer gene (in that case, gene 2) or is it a floor rounding function? (that would truncate the extra %, and round down to gene 1). Or does it pick randomly between the two genes? Knowing this is important! I am assuming here it picks the closer gene, but further tests might reveal the actual rounding method.
| |
| | | | |
| − | ===UPDATE July 16th, 2011===
| + | [[Test of Magnificent Gardens]] |
| − | After scouring past mutagenic notes, its come to my attention that the previous tales mutagenic experts used the ceiling function as their rounding method. I am not sure why they choose this (whether it was a data driven decision or a guess). However, I have re-evaluated some of our Tale 5 data that seemed inconsistent. Specifically, some tests that yielded non-overlapping ranges, given our range method calculations. Using the ceiling function approach gave consistent data! This may be a mistake, but I am updating all my calculations to the Ceiling Function Rounding method of previous tales.
| |
| − | | |
| − | Ceiling Floor Function:
| |
| − | This means that the rounding function will always round UP to the next whole number.
| |
| − | | |
| − | :given a gene target x out of gene length y, the range is:
| |
| − | *minimum range is (x-1)/y x 100%
| |
| − | *maximum range is x/y x 100%
| |
| − | :For example, a mutagen that targets gene 8 out of a possible 27 has minimum range 7/27 x 100% = 25.926% and maximum range of 8/27x100% = 29.629%.
| |
| − | | |
| − | | |
| − | :given a mutagen range, the targeted gene will always be:
| |
| − | *minimum gene is [(minimum range)x(genome length)]/100 rounded up
| |
| − | *maximum gene is [(maximum range)x(genome length)]/100, rounded up
| |
| − | :For example, a mutagen that has range 25.926% to 29.629% targets, on a genome of length 27:
| |
| − | minimum gene is (25.926)x(27)/100 = 7.000002 rounded up to 8
| |
| − | maximum gene is (29.629)x(27)/100 = 7.99983 rounded up to 8.
| |
| − | | |
| − | Please see http://www.atitd.org/wiki/tale4/Mutagens#Advanced_Mutagen_info for more examples.
| |
| − | | |
| − | ==Tayete Recipes==
| |
| − | ===Tay 01===
| |
| − | Dry, Prickly, Reticulated Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 41/Ava 42)
| |
| − | Observed:
| |
| − | :Left Spoint: 1 weed/1 water/1 seed/2 flax/<span style="color:red;">1 rotten flax</span>
| |
| − | :Right Splint: 0 zeed!!
| |
| − | | |
| − | Right splint had no gene groups broken, but gained an additional gene sequence of RRGY
| |
| − | Left splint had the (RO) gene group broken.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *ORR
| |
| − | *GYG
| |
| − | *YRR <-- that pinpoints it as the third G directly after (GYGYGY) as being replaced with an R
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGYGY)<span style="border:1px solid green;">R</span>RGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 11/27.
| |
| − | :gene range is 10/27 to 11/27 or <span style="color:red;">37.037% to 40.740%</span>
| |
| − | :also reveals that a "G" gene was moved to the left splint
| |
| − | | |
| − | :Left Splint:
| |
| − | :The phenotype revealed that the (RO) seed gene was mutated.
| |
| − | :The right gene results show that an "R" gene was switched w/ a "G" gene.
| |
| − | | |
| − | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(<span style="border:1px solid green;">G</span>O)YY(OR)RK
| |
| − | :gene targeted was 21/27
| |
| − | :gene range is 20/27 to 21/27 or <span style="color:red;">74.074% to 77.777%</span>
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Fracture / Crown (Ava 43,44)
| |
| − | :phenotypes: Fracture became giant, colors unchanged. Crown petals went from red to magenta
| |
| − | | |
| − | :Left splint: Fracture: K(ROYG)(ROY(G)YOR)GOR(GOOO)K
| |
| − | :Test 1 predicts gene 7 or 8 will be swapped
| |
| − | : if 7, 1st Y in the "ROYGYOR" set is swapped, it should be a 1 giant + 1 dwarf = small size.
| |
| − | : if 8, then G in middle of "ROYGYOR" set is swapped, both sets are broken, and result is 1 giant.
| |
| − | :observed: its a giant. no color changes
| |
| − | :it was gene 8 swapped.
| |
| − | :range predicted now is 38.888% to 44.444%
| |
| − | :this updates the left splint range to: <span style="color:red;">38.888% to 40.700%</span>
| |
| − | </div>
| |
| − | | |
| − | :Right splint: Crown: K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K
| |
| − | :Test 1 predicts that gene 28 or 29 will swap. (O or 2nd R in the first (UROR) set. If so, a yellow gene will be broken.
| |
| − | :observed: the petals are more magenta colored than red. This is consistent w/ above prediction. It could also be a gene before or after this one that moved, and give the same results, so the exact gene position isn't certain w/o solvent testing.
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: Dusk / Fracture (Ava 72, 73)
| |
| − | :phenotypes: no changes were observed !!
| |
| − | | |
| − | Expected Left: gene 29 or 30. If 30 (GRRO) --> (GRRR)removing Yellow north and adding cyan south
| |
| − | If 29 (GRRO) --> GRRO no change at all.
| |
| − | | |
| − | Expected Right--> 14th gene, R from GOR to swap in. GOR is junk, so no phenotype change is expected.
| |
| − | | |
| − | Results: no differences in phenotypes.
| |
| − | :therefore Left splint targeted gene 29. R-->R.
| |
| − | :genomes are the same.
| |
| − | :range from this is: 28/73 to 29/73 or 38.356% to 39.726%
| |
| − | :updated range is: <span style="color:red;">38.888% to 39.726%</span>
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 4: Vampire / Vampire (Bessodia's Test)
| |
| − | | |
| − | Vampire genome is: KIYIYIOIYIOIOK 12 genes long
| |
| − | Left splint:
| |
| − | *IYIOI
| |
| − | *YOOIY
| |
| − | *YIOIO
| |
| − | *YIYIO
| |
| − | *OOIYI
| |
| − | *IYOOY
| |
| − | *OIOK
| |
| − | *KIYIY
| |
| − | *IYIYO
| |
| − | *YIYOO
| |
| − | | |
| − | an I gene swapped for an O gene:
| |
| − | KIYIY<span style="border:1px solid green;">O</span>OIYIOIOK
| |
| − | 5th gene was targeted.
| |
| − | range is 4/12 to 5/12 or 33.333% to 41.666%
| |
| − | that's consistent w/ the other test results!
| |
| − | | |
| − | Right splint:
| |
| − | *IOIY
| |
| − | *IYIO
| |
| − | *YIII
| |
| − | *IYII
| |
| − | *IIIO
| |
| − | *IYIY
| |
| − | *IIOK
| |
| − | *KIYI
| |
| − | *IIIO
| |
| − | *OIYI
| |
| − | | |
| − | genome is: KIYIYIOIYI<span style="border:1px solid green;">I</span>IOK
| |
| − | 10th gene was targeted.
| |
| − | range is 9/12 to 10/12 or 75.000% to 83.333%.
| |
| − | that narrows the range down, when combined w/ other tests
| |
| − | so the range is now <span style="color:red;">75.000% to 77.777% </span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 5: Crown/blush (Ava 174,175)
| |
| − | | |
| − | Expect: Crown to hit gene 15 or 16 (R,V) and Blush to hit gene 21 (O)
| |
| − | Right splint:
| |
| − | :Observe: Blush lost 1 magenta on middle and top of stamen, and gained 1 cyan on top stamen.
| |
| − | :VIOO --> VIRO gene 20 was picked.
| |
| − | :range is 19/26 - 20/26 or 73.076% to 76.923%
| |
| − | :new range for right splint is <span style="color:red;">75.000% to 76.923% </span>
| |
| − | | |
| − | Left Splint:
| |
| − | :observed no change, so gene 15 was picked.
| |
| − | :gene range is 14/37 to 15/37 or 37.837% to 40.540%.
| |
| − | :this is consistent w/ other tests.
| |
| − | </div>
| |
| − | | |
| − | ===Tay 02 ===
| |
| − | Calico, Green, Striped Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 37/Ava 38)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *RGRO
| |
| − | *GRGY
| |
| − | *ORYY
| |
| − | *ROYY
| |
| − | *GROY
| |
| − | *GRG
| |
| − | *GYOR
| |
| − | *YORY
| |
| − | *YGYG
| |
| − | no anomolies found yet. Standard Junk genes all covered
| |
| − | | |
| − | :Solvents on Left Splint showed:
| |
| − | *GRGY
| |
| − | *ORYY
| |
| − | *YGRG
| |
| − | *GROY
| |
| − | *RGRO
| |
| − | *ORGR
| |
| − | *GYGY
| |
| − | *YGYG
| |
| − | *GYOR
| |
| − | *YRG
| |
| − | *ORRK
| |
| − | | |
| − | no anomolies found, and standard junk genes all covered.
| |
| − | :this test is inconclusive
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Silken / Vampire (Ava 26, 27)
| |
| − | :phenotypes: Silken bulb unchanged, Vampire had center go magenta (ie had an IO get broken)
| |
| − | | |
| − | :Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK
| |
| − | :Right splint: Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K
| |
| − | Left Splint:
| |
| − | *IIIK
| |
| − | *RRRO
| |
| − | *UIII
| |
| − | *YYYG
| |
| − | *YYYY <-- pinpoints as the last O --> G
| |
| − | genome is KRRROO<span style="border:1px solid green;">Y</span>YYYGGGUUUIIIK
| |
| − | 6th out of 18 gene targeted
| |
| − | range is 5/18 to 6/18 which is <span style="color:red;">27.777% to 33.333%</span>
| |
| − | | |
| − | Right Splint:
| |
| − | know from phenotype we lost an IY, know from above we had Y-->O. so we trade IY for IO.
| |
| − | *YIOI
| |
| − | *KIYI
| |
| − | *IOIO
| |
| − | *IOIYI <-- pinpoints as the second Y
| |
| − | genome is KIYI<span style="border:1px solid green;">O</span>IOIYIOIOK
| |
| − | 4th gene out of 12
| |
| − | range is 3/12 to 4/12 which is <span style="color:red;">25.000% to 33.333%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: Vampire/ Vampire (Bessodia's Test)
| |
| − | Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K
| |
| − | | |
| − | no visual different between the flowers.
| |
| − | solvent testing showed no changes.
| |
| − | presuming the same gene type swapped.
| |
| − | No conclusions available.
| |
| − | </div>
| |
| − | | |
| − | TEST 4: crown/vampire (ava 188, Ava 189)
| |
| − | | |
| − | Left:
| |
| − | :expected to hit gene 11 -13 (OOV)
| |
| − | :observed: Mag W was broken. So it was gene 11 or 12 (the O's in UROO
| |
| − | :range is 10/32 to 12/32 = 27.027% - 32.432%
| |
| − | :new left range is <span style="color:red;">27.333% to 32.432%</span>
| |
| − | | |
| − | Right:
| |
| − | :expected to hit gene 4 or 5 (Y OR I)
| |
| − | :observed: IY broke and got IO, so it was gene 4 that ws hit
| |
| − | :range is 3/12 to 4/12 or 25.000% to 33.333%.
| |
| − | :This is consistent w/ previous tests.
| |
| − | </div>
| |
| − | | |
| − | ===Tay 03 ===
| |
| − | Calico, Mottled, slimy Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green (Ava 35, Ava 36)
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :Left Splint: 1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | :Right Splint: 1 weed/01 water/1 seed / 2 flax/ 0 rotten
| |
| − | | |
| − | Both splints had junk genes swapped.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *GGYG <-- that pinpoints it as 12th gene, R being replaced with G
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGYG)G<span style="border:1px solid green;">G</span>)GY(OR)YYRG(OR)YY(OR)RK
| |
| − | :gene targeted was 12/27.
| |
| − | :gene range is 11/27 to 12/27 or <span style="color:red;">40.749% to 44.444%</span>
| |
| − | :also reveals that a "R" gene was moved to the left splint
| |
| − | | |
| − | Left Splint:
| |
| − | :Looking for where a junk G was replaced with a R gene, and no new genes were created. (Phenotype was not altered)
| |
| − | Solvents on Left Splint showed:
| |
| − | *ORGR
| |
| − | *GYGY
| |
| − | *YGYG
| |
| − | *YORY
| |
| − | *RYYR
| |
| − | *ROYY
| |
| − | *RGR
| |
| − | *GYOR
| |
| − | *RGYO
| |
| − | *YYO
| |
| − | *YGY
| |
| − | *RGYG
| |
| − | *YRR <--- confirms G --> R switch. Only two places it could be. 11th or 20th.
| |
| − | *RRRO <-- proves 20th gene was swapped to R.
| |
| − | | |
| − | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYR<span style="border:1px solid green;">R</span>(RO)YY(OR)RK
| |
| − | :gene targeted was 20/27
| |
| − | :gene range is 19/27 to 20/27 or <span style="color:red;">70.370% to 74.074%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Silken / Clarity (Ava 28, Ava 29)
| |
| − | :phenotypes: Silken bulb unchanged, Clarity had a cyan gene broken.
| |
| − | | |
| − | :Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK
| |
| − | :left target 13 or 14 /18 gene --> 1st or 2nd U.
| |
| − | | |
| − | :Right splint: Clarity Genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GORR)GRORGRROK
| |
| − | :right target is 15 or 16/36 gene --> 1sr or 2nd R in the first cyan gene set (GORR)
| |
| − | Solvent:
| |
| − | *IRIR
| |
| − | *RIOI
| |
| − | *OIYI
| |
| − | *UGRO <-- pinpoints as the 2nd R in (GORR), and the 16th gene
| |
| − | 16 gene out of 36
| |
| − | range is 15/36 to 16/36 or <span style="color:red;">41.667% to 44.444%</span>
| |
| − | :resultant right genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GOR<span style="border:1px solid green;">U</span>)GRORGRROK
| |
| − | | |
| − | Left Splint Solvent:
| |
| − | *GGGR. That confirms it.
| |
| − | :we know now what left genome is, since it's U was replaced with an R from Clarity:
| |
| − | :resultant left genome: KRRROOOYYYGGG<span style="border:1px solid green;">R</span>UUIIIK
| |
| − | :13th gene targeted.
| |
| − | :range is 12/18 - 13/18 = 66.667% to 72.222%
| |
| − | Combining with Test 1 results, new Left range is <span style="color:red;">70.370% to 72.222%</span>
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: Vampire/ Vampire (Bessodia's Test)
| |
| − | Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K
| |
| − | | |
| − | Left Splint:
| |
| − | *YOOI
| |
| − | *IOIY
| |
| − | *IYIO
| |
| − | *IYOO
| |
| − | *YIYI
| |
| − | *IYIY
| |
| − | *OOIO
| |
| − | *OIYO
| |
| − | *YIOI
| |
| − | *IOIK
| |
| − | *KIYI
| |
| − | | |
| − | 9th gene was swapped: : K(IY)(IY)(IO)(IY)<span style="border:1px solid green;">O</span>O(IO)K
| |
| − | range is 8/12 to 9/12 = 66.667% to 75.000%.
| |
| − | That is consistent w/ other tests.
| |
| − | | |
| − | Right Splint:
| |
| − | *IYII
| |
| − | *IIIY
| |
| − | *IOIK
| |
| − | *IIYI
| |
| − | *KIYI
| |
| − | *YIOI
| |
| − | 6th gene was swapped: KIYIYI<span style="border:1px solid green;">I</span>IYIOIOK
| |
| − | range is 41.667% to 50.000%.
| |
| − | This is consist with other Test findings.
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 4: Crown / Fracture
| |
| − | MEANT to do crown / clarity....oops!
| |
| − | | |
| − | yielded Ava 121, Ava 122
| |
| − | Left: Broke UROR (Yellow West). Not sure if gene 27 (R) or gene 28(O) w/o using solvents.
| |
| − | | |
| − | Right: (ROYG)(ROY_YOR)GOR(GOO)
| |
| − | broke that middle _ gene (was a G). it broke up a giant and a dwarf gene, leaving just 1 giant gene in the genome.
| |
| − | can't tell what was swapped in, w/o solvent.
| |
| − | | |
| − | test inconclusive, w/o solvents.
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 5: Crown/Clarity (Ava 129. Ava 130)
| |
| − | | |
| − | expected: Crown's gene 27 or 28 ... UROR... (the RO in the middle)
| |
| − | expected: Clarity's genes 15, 16, 17.
| |
| − | gene 15, 16 are R's in GORR
| |
| − | gene 17 is G in GROR
| |
| − | | |
| − | Observed: phenotypes of flowers are the same!!
| |
| − | so both splints swapped R's.
| |
| − | | |
| − | Left: gene 27 of 37 was targeted
| |
| − | range is 26/37 to 27/37 or 70.270% o 72.975%.
| |
| − | this is consistent w/ other tests, but a larger range.
| |
| − | | |
| − | Right Splint:
| |
| − | was gene 15 or 16 of 36 total
| |
| − | range is 14/36 to 16/36 or 38.888% to 44.444%
| |
| − | this is consistent w/ other tests, but a larger range.
| |
| − | | |
| − | ===Tay 04 ===
| |
| − | Cracky, Prickly, Spongy Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 49/Ava 50)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *not tested yet
| |
| − | | |
| − | :Solvents on Left Splint showed:
| |
| − | *KORG
| |
| − | *ORGR
| |
| − | *RYY
| |
| − | *YGY, GYG
| |
| − | *YORY
| |
| − | *GROY
| |
| − | *ORYY
| |
| − | *GYGY
| |
| − | *RGRO
| |
| − | *YYRG
| |
| − | *YRGR
| |
| − | *GYGR
| |
| − | *RGYO
| |
| − | | |
| − | no anomolies found, and standard junk genes all covered.
| |
| − | Seems that the <span style="color:red;">Same Gene type</span> was swapped between splints :(
| |
| − | further tests are needed to characterize this mutagen
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Vampire / vampire (Bessodia's Test)
| |
| − | Vampire: KIYIYIOIYIOIOK 12 genes
| |
| − | | |
| − | Right splint:
| |
| − | *IYIO
| |
| − | *YYIO
| |
| − | *YIOI
| |
| − | *KIYY
| |
| − | *IYYY
| |
| − | *IOIY
| |
| − | *OIYI
| |
| − | *IYYY
| |
| − | *0IOK
| |
| − | 3rd gene was swapped: KIY<span style="border:1px solid green;">Y</span>YIOIYIOIOK
| |
| − | range is 2/12 to 3/12 which is <span style="color:red;">16.667% to 25.000%</span>
| |
| − | | |
| − | Left Splint:
| |
| − | *KIII
| |
| − | 2nd gebe was swapped. KI<span style="border:1px solid green;">I</span>IYIOIYIOIOK
| |
| − | range is 1/12 to 2/12 which is <span style="color:red;">8.333% to 16.667%</span>
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: Blush/morning (Ava 170, Ava 171)
| |
| − | | |
| − | :expect gene 3,4, 5 on blush (RVG)
| |
| − | :expect gene 9,10,11,12,13 on morning (IYYYU)
| |
| − | | |
| − | Observe: Left lost Mn, so it was Gene 3 targeted!
| |
| − | Right lost 1 yellow middle and lower stamen, so it was gene 11 targeted.
| |
| − | | |
| − | :Left had gene 3 of 26 picked
| |
| − | :range is 2/26 to 3/26 or 7.692% to 11.538%
| |
| − | :new range is <span style="color:red;">8.333% to 11.538%</span>
| |
| − | | |
| − | :RIght had gene 11 of 44 picked
| |
| − | :range is now <span style="color:red;"> 10/44 to 11/44 or 22.727% to 25.000%</span>
| |
| − | | |
| − | ===Tay 05 ===
| |
| − | Crackly, Dry, spongy Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 39/Ava 40)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | Solvents on Right Splint showed:
| |
| − | *not tested yet
| |
| − | | |
| − | :Solvents on Left Splint showed:
| |
| − | *GYGR
| |
| − | *RYYR
| |
| − | *YORR
| |
| − | *GROY
| |
| − | *OYY
| |
| − | *YRG
| |
| − | *RGR
| |
| − | *GYOR
| |
| − | *RGYO
| |
| − | *GYG
| |
| − | *OYYO
| |
| − | *ROYY
| |
| − | *GRGY
| |
| − | | |
| − | no anomolies found, and standard junk genes all covered.
| |
| − | Seems that the <span style="color:red;">Same Gene type</span> was swapped between splints :(
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Blush / Morning (Ava 31, 32)
| |
| − | :phenotypes: Both phenotypes were unchanged
| |
| − | :Left splint: Blush Genome: KGRORVGOOOVUROOVIOVIOOVIOOOK
| |
| − | :Right splint: Morning: KIRRRIYYYIYYYUYYYUYYYURORURORGRRRGYYYGORRGRROK
| |
| − | morning has no junk genes, therefore the same type of gene must have been swapped.
| |
| − | :so far, no conclusions made
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: Vampire / vampire (Bessodia's Test)
| |
| − | Vampire: KIYIYIOIYIOIOK 12 genes
| |
| − | | |
| − | Right splint:
| |
| − | *IIOI
| |
| − | *IIIO
| |
| − | *IYIY
| |
| − | *IYIO
| |
| − | *IOIO
| |
| − | *YIYI
| |
| − | *IOII
| |
| − | *KIYI
| |
| − | *OIOK
| |
| − | 8th gene was swapped: KIYIYIOI<span style="border:1px solid green;">I</span>IOIOK
| |
| − | range is 7/12 to 8/12 which is <span style="color:red;">58.333% to 66.667%</span>
| |
| − | | |
| − | Left Splint:
| |
| − | *YIYI
| |
| − | *IOYY
| |
| − | *YIOI
| |
| − | *OIOK
| |
| − | *IOYY
| |
| − | *KIYI
| |
| − | *OYYI
| |
| − | | |
| − | 7th gene was swapped. KIYIYIO<span style="border:1px solid green;">Y</span>YIOIOK
| |
| − | range is 6/12 to 7/12 which is <span style="color:red;">50.000% to 58.333% </span>
| |
| − | </div>
| |
| − | | |
| − | div style="margin:30px">
| |
| − | TEST 4: Fracture/Vampire (Ava 176, Ava 177)
| |
| − | | |
| − | Observed:
| |
| − | :Left: broken dwarf, but kept 2 giant gene sets. So it targetd gene 9, 10, 11 (YOR)
| |
| − | :Right: got an IO gene and lost an IY gene --> lost an O, and gained aY
| |
| − | | |
| − | :Left splint had gene 10 targeted (the Y) of 18
| |
| − | :left range is 50.000% to 55.555%
| |
| − | :this is a new range!
| |
| − | | |
| − | :right splint had gene 8 picked, of 12
| |
| − | :gene range is 7/12 to 8/12, or 58.333 to 66.666%
| |
| − | :this is the same range as above.
| |
| − | </div>
| |
| − | | |
| − | ===Tay 06===
| |
| − | Fuzzy, Hairy, SPongy, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 33/Ava 34)
| |
| − | Observed:
| |
| − | :Left Spoint: 1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | :Right Splint: 2 weed/0 water/1 seed / 1 flax/ 0 rotten
| |
| − | | |
| − | Right splint was affected in (GYGYGY) gene, specifically NOT the middle GY genes.
| |
| − | Left splint had a junk gene swapped.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *RYY
| |
| − | *RGYG
| |
| − | *GGRG <-- that pinpoints it as the third Y in (GYGYGY) being replaced with a G
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGYG<span style="border:1px solid green;">G</span>)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 10/27.
| |
| − | :gene range is 9/27 to 10/27 or <span style="color:red;">33.333% to 37.037%</span>
| |
| − | :also reveals that a "Y" gene was moved to the left splint
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *KOR
| |
| − | *ORRK
| |
| − | *RGY
| |
| − | *YYOR
| |
| − | *ROOY
| |
| − | *ORYY
| |
| − | *GYY <--- proves it
| |
| − | :Looking for where a junk G was replaced with a Y gene, and no new genes were created. (Phenotype was not altered)
| |
| − | | |
| − | Left Genome: K(OR)GR(GYGYGY)<span style="border:1px solid green;">Y</span>RGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 11/27
| |
| − | :gene range is 10/27 to 11/27 or <span style="color:red;">37.037% to 40.740%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Ava 45 / Blush
| |
| − | | |
| − | This is Step #2 in section below, trying for 75% green flower.
| |
| − | Left: (UYYY) yellow gene was broken on Ava 45.
| |
| − | need to do solvent testing to know which gene it was exactly.
| |
| − | | |
| − | Right: predicted either gene 9 or 10 to swap.
| |
| − | :gene 9 would have broken (GOOO)
| |
| − | :Gene 0 is a V. no phenotype change expected.
| |
| − | No phenotype change of blush was seen. Therefore, we know 10th gene V swapped.
| |
| − | 10/26 gene targeted
| |
| − | range is 9/26 to 10/26 = 34.615% to 38.461%
| |
| − | combining with test 1, that gives overlap range of <span style="color:red;">34.615% to 37.037%.</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Vamp/Crown (Ava 182, Ava 183)
| |
| − | | |
| − | Expect Vamp to donate gene 5, I... and Crown to hit 13 or 14, V or U
| |
| − | | |
| − | :Observed: Crown gained an IR gene (and vamp lost an IO).
| |
| − | :therefore, crown was hit at gene 14, U--I.
| |
| − | | |
| − | Right splint: gene 14 of 37 was targeted
| |
| − | :range is 13/37 to 14/37 or 35.135% to 37.837%
| |
| − | :new range for right splint is <span style="color:red;">35.135% to 37.037%</span>
| |
| − | Left splint: gene 5 was targeted.
| |
| − | :range is 4/12 to 5/12, or 33.333% to 41.666%
| |
| − | :This is consistent with above tests.
| |
| − | </div>
| |
| − | | |
| − | ===Tay 07===
| |
| − | Crackly, Hairy, Prickly Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 51/Ava 52)
| |
| − | Observed:
| |
| − | :Left Spoint: 2 weed/1 water/1 seed/2 flax/0 rotten had an extra weed!
| |
| − | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten not changed
| |
| − | | |
| − | Left splint was affected in (OR) gene.
| |
| − | Right splint had a junk gene swapped.
| |
| − | | |
| − | Solvents on Left Splint (Ava51) showed:
| |
| − | *YYOY <-- that pinpoints it as the last (OR) broken. R was replaced w/ Y.
| |
| − | :Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(O<span style="border:1px solid green;">Y</span>))RK
| |
| − | :gene targeted was 26/27
| |
| − | :gene range is 25/27 to 26/27 or <span style="color:red;">92.592% to 96.296%</span>
| |
| − | | |
| − | Right splint had a junk Y replaced.
| |
| − | Solvents on Right Splint showed:
| |
| − | *GRGY
| |
| − | *GROY
| |
| − | *GYGY
| |
| − | *RGRO
| |
| − | *RGRG
| |
| − | *RRK
| |
| − | *YRG
| |
| − | *YYO
| |
| − | *YOR
| |
| − | *ORG
| |
| − | *ORR <--- pinpoints it (after other solvents eliminated some possibly Y's).
| |
| − | The 17th Y gene is swapped out and was replaced with an R
| |
| − | :Right genome: K(OR)GR(GYGYGY)GRGY(OR)<span style="border:1px solid green;">R</span>YRG(RO)YY(OR))RK
| |
| − | :gene targeted was 17/27
| |
| − | :gene range is 16/27 to 17/27 or <span style="color:red;">59.259% to 62.963%</span>
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Clarity / Morning
| |
| − | (Ava 69, Ava 70)
| |
| − | | |
| − | Left: this will swap either the 34th or 35th gene in Clarity. Both are R genes.
| |
| − | :.... GRROK.
| |
| − | | |
| − | Right: this will swap either the 27, 28 or 29 gene (of 44) in Morning.
| |
| − | :original genome: ...(UROR)(GRRR)...
| |
| − | if 27th, (UROR) --> (URRR). This will remove Yellow Inner West and add Cyan Inner East.
| |
| − | if 28th (UROR) ---> (UROR). No change in genome or phenotype
| |
| − | if 29th (GRRR)--> RRRR. this removes cyan from the outer south, and adds nothing.
| |
| − | | |
| − | Observed: Yellow inner west was removed and cyan inner east was added.
| |
| − | Right genome:KIRRRIYYYIYYYUYYYUYYY(UROR)(UR(O<span style="border:1px solid green;">R</span>)R)GRRRGYYYGORRGRROK
| |
| − | :gene targeted was 27/44.
| |
| − | :gene range is: 26/44 to 27/44 or 60.227% to 62.500%.
| |
| − | :combined with TEST 1's results, Right splint range is now: <span style="color:red;">59.259% to 61.363%</span>
| |
| − | | |
| − | Left: GRRO gene was indeed broken, removing a yellow and leaving, in total, 1 black and 1 blue color on the Outer North Petals.
| |
| − | No further conclusions can be made about which R gene was swapped.
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: Vampire/vampire (Bessodia's Test)
| |
| − | Left genome: IYIYIOIOIOIO.
| |
| − | 8th gene 0-->Y
| |
| − | range is 58.333% to 66.666%
| |
| − | this is wider than already known range.
| |
| − | | |
| − | Right genome: IYIYIOIYIOIY
| |
| − | 12th gene Y-->O
| |
| − | range is 91.666% - 100%
| |
| − | this is wider than already known range.
| |
| − | </div>
| |
| − | | |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 4: Crown / Fracture (Ava 125, Ava 126)
| |
| − | | |
| − | expect: gene 35 or 36 in crown (center RO in UROR gene).
| |
| − | Fracture just donates an R.
| |
| − | | |
| − | Observed:
| |
| − | Left has 1 cyan east and lost 1 yellow west.
| |
| − | so gene 36 was swapped .. UROR --> URRR
| |
| − | gene 36/37 targeted
| |
| − | genome: UROO UROO UROO V URVOOVURVORV UROR UROR UR<span style="border:1px solid green;">R</span>R
| |
| − | | |
| − | range is 35.5/37 to 36.5/37 or 95.946% to 98.649%
| |
| − | combining with other test results, new range is <span style="color:red;">95.946% to 98.143%</span>
| |
| − | | |
| − | Right: colors are the same, but its a giant x 2!!
| |
| − | we know it was an R being swapped out with an O.
| |
| − | genome: ROYG ROYGYO<span style="border:1px solid green;">O</span> GOR GOOO
| |
| − | gene 11/18 was targeted
| |
| − | range is 10.5/18 to 11.5/18 or 58.333% to 63.889%
| |
| − | This is wider than our current range.
| |
| − | | |
| − | </div>
| |
| − | | |
| − | ===Tay 08 ===
| |
| − | Crackly, Phosphorescent, Slimy Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 53/Ava 54)
| |
| − | Observed:
| |
| − | :Left Spoint: 0 weed/1 water/1 seed/0 flax/0 rotten ... no weed and no flax are different
| |
| − | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step
| |
| − | | |
| − | Left splint was affected in ROY sequence (which is required to harvest flax, see flax genome theories for more info). However, it was not a *(RO) that was changed, or the seed count would have gone down. Furthermore, we need a new OR gene, to account for the 1 less weed step.
| |
| − | :looks like the 2nd to last Y should be switched to a R. ... YYRG(RO)RY(OR)RK. This would add (OR) and removed the (ROY).
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *GYG
| |
| − | *RGR
| |
| − | *ROR <-- that pinpoints it as the gene we suspected.
| |
| − | :the 23rd gene, Y, is replaced with an R
| |
| − | :gene range is 22/27 to 23/27 or <span style="color:red;">81.481 to 85.185%%</span>
| |
| − | :Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)<span style="border:1px solid green;">R</span>Y(OR)RK
| |
| − | | |
| − | Right splint lost an (-1 weed) gene, so (OR) was broken somewhere in the genome.
| |
| − | We know a R was replaced with a Y, from the left splint tests.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *ORY
| |
| − | *ROY
| |
| − | *GRGY
| |
| − | *GYGY
| |
| − | *YOR
| |
| − | *ROYY
| |
| − | *YYRG
| |
| − | *RGR
| |
| − | *OYYO
| |
| − | *YORY
| |
| − | *KOY <--- shows its the second gene, R, that is swapped with a Y from the other splint
| |
| − | :gene targeted was 2/27.
| |
| − | :gene range is 1/27 to 2/27 or <span style="color:red;">3.703% to 7.407%</span>
| |
| − | :Right Genome: K(O<span style="border:1px solid green;">Y</span>)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Crown / Ava 28 (Ava 123, Ava 124)
| |
| − | | |
| − | Expected: Crown will have gene 31 or 32 targeted (center RO in UROR gene set)
| |
| − | If 31, there will be no color change (R --> R)
| |
| − | If 32, we will lose Ywest and gain Ceast (UROR --> URRR)
| |
| − | | |
| − | Ava 28 is just donating an R from position 1 or 2.
| |
| − | | |
| − | Observed:
| |
| − | Left: Cyan East is present, and one Yellow west was lost.
| |
| − | so Gene 32 was targeted.
| |
| − | Genome: UROOUROOUROOVURVOOVURVORVUROR UR<span style="border:1px solid green;">R</span>R UROR
| |
| − | range is 31/37 to 32/37 or 85.135% to 87.037%
| |
| − | combining with other test results, the range is now <span style="color:red;">85.135% to 85.185%</span>
| |
| − | | |
| − | Right: original genome of Ava 28: KRRROOYYYYGGGUUUIIIK
| |
| − | either gene 1 or 2 was swapped, so resulting genome is:
| |
| − | KORROOYYYYGGGUUUIIIK OR KROROOYYYYGGGUUUIIIK.
| |
| − | Can't tell which w/o solvent test.
| |
| − | so this splint info is inconclusive.
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: vampire/crown (Ava 180, Ava 181)
| |
| − | | |
| − | Expect: vampire to donate gene 10, O
| |
| − | :crown will hit gene 2 or 3, RO in URRO
| |
| − | :if gene 2, vamp loses IO, gains IR/crown loses Mw, gains Ms
| |
| − | :if gene 3, O changes with O, no changes
| |
| − | | |
| − | Observed:
| |
| − | :left, vampire gained magenta on middle and lower stamen, lost mage on upper stamen
| |
| − | :right, crown gained IO, lost Mw
| |
| − | | |
| − | :Left was gene 11! IYIYIOIYIOOO now.
| |
| − | :gene was 11 / 12
| |
| − | :gene range is 10/12 to 11/12 or 83.333% to 91.667%
| |
| − | this is consistent w/ range.
| |
| − | | |
| − | :RIght was gene 3 of 37
| |
| − | :gene range is 2/37 to 3/37, or 5.405% to 8.108%
| |
| − | :right range is now <span style="color:red;">5.405% to 7.407%</span>
| |
| − | </div>
| |
| − | | |
| − | ===Tay 09 ===
| |
| − | Calico, Green, Smelly Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 55/Ava 56)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *GRG
| |
| − | *GRO
| |
| − | *GYGY
| |
| − | *YGYG
| |
| − | *ORYY
| |
| − | *ORGR
| |
| − | *YORY
| |
| − | *YYOR
| |
| − | *RGYO
| |
| − | *OYYO
| |
| − | *GYGR
| |
| − | *RYY
| |
| − | *YRGR
| |
| − | *ORRK
| |
| − | no anomolies found yet. Standard Junk genes all covered.
| |
| − | | |
| − | This mutation swapped same gene type between splints
| |
| − | :this test is inconclusive
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Vampire/Vampire (Bessodia's Test)
| |
| − | | |
| − | R: KIYOYIOIYIOIOK
| |
| − | 3rd gene was switched
| |
| − | range is 2/12 to 3/12 or <span style="color:red;">16.666% to 25.000%</span>
| |
| − | | |
| − | Left: KIYIYIIIYIOIOK
| |
| − | 6th gene was switched
| |
| − | range is 5/12 to 6/12 or <span style="color:red;">41.667% to 50.000%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: Silken/Blush
| |
| − | Ava 24/Ava25
| |
| − | | |
| − | observed: blush lost magenta south, silken still white
| |
| − | | |
| − | Right Splint (Ava 25)
| |
| − | original genome is blush:GRORVGOOOVUROOVIOVIOOVIOOO
| |
| − | expected target range is gene 5 to 7
| |
| − | Solvent:
| |
| − | *VURO
| |
| − | *VYOO
| |
| − | *OOVU
| |
| − | *OVIO
| |
| − | *OOVI
| |
| − | *ORVY
| |
| − | 6th gene was target G --> Y
| |
| − | correct genome is:GRORV <span style="border:1px solid green;">Y</span> OOOVUROOVIOVIOOVIOOO
| |
| − | range is 5/26 to 6/26 or <span style="color:red;">19.230% to 23.077</span>, a new range
| |
| − | | |
| − | Left Splint (Ava 25)
| |
| − | original genome is silken: RRROOOYYYGGGUUUIII
| |
| − | we know it gave a Y to other splint and got a G
| |
| − | Solvent:
| |
| − | *UUII
| |
| − | *UIII
| |
| − | *OOYY
| |
| − | *KRRR
| |
| − | *GGGG <-- pinpoints as the last Y being swapped for a G
| |
| − | gene is 9th of 18
| |
| − | range is 8/18 to 9/18 or <span style="color:red;">44.444% to 50.000%</span>, a new range.
| |
| − | correct genome is: RRROOOYY<span style="border:1px solid green;">G</span>GGGUUUIII
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | Range on right splint narrowed down to <span style="color:red;">20.454% to 22.727%</span>
| |
| − | See Test 2 under section for getting 2 shades of cyan on inner stamen
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 5: Clarity/Crown (Ava 200, Ava 201)
| |
| − | | |
| − | :expect clarity to hit gene 17-19 (GRO in GROR) and crown to hit gene 8 or 9 (last O in UROO or U in UROO)
| |
| − | :observed clarity broke Mn and Crown broke Mw and gained Yw.
| |
| − | :this means left hit 18 (GROR -->GOOR) and right hit 8 (UROO --> UROR)
| |
| − | | |
| − | :Left splint targeted gene 18 of 36
| |
| − | :range is 17/36 to 18/36 or a new range of <span style="color:red;">47.222% to 50.000%</span>
| |
| − | :Right splint targeted gene 8 of 37
| |
| − | :range is 7/37 to 8/37 or 18.91% to 21.621%
| |
| − | :new range of right is <span style="color:red;">20.454% to 21.621%</span>
| |
| − | </div>
| |
| − | | |
| − | ===Tay 10===
| |
| − | Crackly, Spotted, Striped , Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 57/Ava 58)
| |
| − | Observed:
| |
| − | :Left Spoint: 2 weed/0 water/1 seed/0 flax/0 rotten
| |
| − | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten
| |
| − | | |
| − | Left splint was affected in (GYGYGY) gene, specifically in the middle GY genes.
| |
| − | This breaks up both Flax genes (GYGY) as well as the water gene (GYGYGY), which would account for our observations.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *YGRG <-- that pinpoints it as the middle Y in (GYGYGY) being replaced with a R
| |
| − | :Left Genome: K(OR)GR(GYG<span style="border:1px solid green;">R</span>GY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 8/27.
| |
| − | :gene range is 7/26 to 8/27 or <span style="color:red;">25.925% to 29.629%</span>
| |
| − | | |
| − | Right splint had a -1 weed gene broken (OR). From above, we know it was the R that was swapped with a Y from the Left splint.
| |
| − | Just have to identify which of the three (OR) genes was broken.
| |
| − | Solvents on Right Splint showed:
| |
| − | *RGRG
| |
| − | *RGY
| |
| − | *GYG
| |
| − | *YRGY
| |
| − | *RGY
| |
| − | *ORG
| |
| − | *YGY
| |
| − | *ORY
| |
| − | *RGY
| |
| − | *YOR
| |
| − | *YYR
| |
| − | *RRY
| |
| − | *GYO
| |
| − | *OYRB <--- pinpoints the (OR) gene at the end as switching to (OY).
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(O<span style="border:1px solid green;">Y</span>)RK
| |
| − | :gene targeted was 26/27
| |
| − | :gene range is 25/27 to 26/27 or <span style="color:red;">92.592% to 96.296%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: morning / Ava 28
| |
| − | yields flowers Ava 103, Ava 104
| |
| − | | |
| − | Left:
| |
| − | Above test 1 predicts gene 12-14 will be chosen, and that Ava 28 will donate a "I" gene
| |
| − | morning genome: K IRRR IYYY IYYY UYYY ...
| |
| − | if 12th gives ... IYYI UYYY... would lose 1 yellow on inner stamen
| |
| − | if 13th, gives ... IYYY IYYY... would lose 1 yellow East, gain 1 yellow on entire stamen
| |
| − | if 14th, gives ... IYYY UIYY... would lose 1 yellow East, gain 1 yellow on outer and middle stamen
| |
| − | | |
| − | Observed: top and middle staymen are same. Inner stamen is more blueish. East petals are the same.
| |
| − | therefore, the 12th gene was exchanged.
| |
| − | so the range is 11/44 to 12/44 or 25.000% to 27.272%
| |
| − | combining that with Test 1 results, the new range is <span style="color:red;">25.925% to 27.272%</span>
| |
| − | | |
| − | Right:
| |
| − | Test 1 predicted that gene 17 or 18 would be switched.
| |
| − | Ava 28 genome is: KRRROOOYYYGGGRUUIIIK
| |
| − | Either is an I gene.
| |
| − | if 17th, gives ...UUIYIK would introduce yellow on the top stamen
| |
| − | if 18th, gives ...UUIIYK would still appear all white.
| |
| − | | |
| − | Observed: the top stamen has 1 shade of yellow now.
| |
| − | therefore, the 17th gene was swapped.
| |
| − | the range is 16/18 to 17/18 or 88.888% to 94.444%
| |
| − | combining hat with Test 1 results, the new range is <span style="color:red;">92.592% to 94.444%</span>
| |
| − | </div>
| |
| − | | |
| − | TEST 3: Vampire/Vampire (Bessodia's Test)
| |
| − | | |
| − | Left: KIYIOIOYIOIOK
| |
| − | 4th gene swapped
| |
| − | range is 3/12 to 4/12 or 25.000% to 33.333%
| |
| − | consistent w/ above tests.
| |
| − | | |
| − | Right: KIYIYIOIYIOIK
| |
| − | 12th gene is switched
| |
| − | range is 91.667% to 100%
| |
| − | consistent w/ above tests.
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 4: crown/Ava 24 (for silken) (Ava 186, Ava 187)
| |
| − | | |
| − | Left:
| |
| − | :Expected: gene 10 or 11 to be hit, R and O in UROO.
| |
| − | :observed: Mw was broken, but gained an IOO.
| |
| − | :Gene 10 must have been hit, UROO --> UIOO.
| |
| − | :range is 9/37 to 10/37 or 24.324% to 27.027%
| |
| − | :new range for left splint is <span style="color:red;">25.925% to 27.027%</span>
| |
| − | | |
| − | Right:
| |
| − | :Expected gene 17 to swap, the middle I.
| |
| − | :observed: gained an IR. consistent w/ above. I-->R on middle I gene.
| |
| − | :Gene 17 of 18 was hit.
| |
| − | :range is 16/18 to 17/18 or 88.888% to 94.444%
| |
| − | :new range for right splint is <span style="color:red;">92.592% to 94.444%</span>
| |
| − | </div>
| |
| − | | |
| − | ===Tay 11 ===
| |
| − | Phosphoresecent, Slimy, Smelly Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 59/Ava 60)
| |
| − | Observed:
| |
| − | :Left Spoint: same phenotype
| |
| − | :Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step
| |
| − | | |
| − | Right splint had an extra weeding step. This means an (OR) gene was broken up.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *YRRR <-- that pinpoints it as the last O gene --> R gene.
| |
| − | :the 25nd gene, O, is replaced with an R
| |
| − | :gene range is 24/27 to 25/27 or <span style="color:red;">88.888% to 92.592%</span>
| |
| − | :Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(<span style="border:1px solid green;">R</span>R)RK
| |
| − | | |
| − | Left splint lost an R gene in the junk genes somewhere, which was replaced with an O gene.
| |
| − | Solvents on Left Splint showed:
| |
| − | *YGRG
| |
| − | *OGRO <--- shows its the 19th gene, R, that is swapped with a G from the other splint.
| |
| − | :gene targeted was 19/27.
| |
| − | :gene range is 18/27 to 19/27 or <span style="color:red;">66.666% to 70.370%</span>
| |
| − | :Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YY<span style="border:1px solid green;">O</span>G(RO)YY(OR)RK
| |
| − | </div>
| |
| − | | |
| − | ===Tay 12 ===
| |
| − | Calico, Crackly, Prickly Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 61/Ava 62)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *ORYY
| |
| − | *GYGR
| |
| − | *ROYY
| |
| − | *ORGR
| |
| − | *YRGR
| |
| − | *YYOR
| |
| − | *RGYG
| |
| − | *RGRG
| |
| − | *GYGY
| |
| − | *GRGY
| |
| − | *GYO
| |
| − | *RGRO
| |
| − | *RRK
| |
| − | no anomolies found yet. Standard Junk genes all covered.
| |
| − | | |
| − | This mutation swapped same gene type between these splints.
| |
| − | :This test is inconclusive
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Vampire/Vampire (Bessodia's Test)
| |
| − | | |
| − | Left: KIOIYIOIYIOIOK
| |
| − | 2nd gene switched
| |
| − | range is 1/12 to 2/12 or <span style="color:red;">8.333% to 16.667%</span>
| |
| − | | |
| − | Right: KIYIYIYIYIOIOK
| |
| − | 6th gene switched
| |
| − | range is 5/12 to 6/12 or <span style="color:red;">41.667% to 50.000%</span>
| |
| − | | |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: Ava 25/Ava 25
| |
| − | | |
| − | Ava 25 flower is blush with 1 mutated gene: GROR V YOOO V UROO V IO V IOO V IOOO
| |
| − | Expected range: left gene 3-5 (O,R,V) , right gene 12-14 (R,O,O)
| |
| − | | |
| − | Ava 133 / Ava 134 result
| |
| − | | |
| − | Observed:
| |
| − | Left: Magenta west, all other petals are white. therefore, GROR was broken
| |
| − | Right: Magenta north, all other petals are white. Therefore, UROO was broken
| |
| − | | |
| − | Left Analysis:
| |
| − | was not gene 5 (V) or there would have been no phenotype change.
| |
| − | was not gene 3(O) or would be either GROR or GRRR, both are colors that are not present.
| |
| − | it was gene 4 (R) of 26 total.
| |
| − | range is 3/26 to 4/26 or <span style="color:red;">11.538% to 15.385%</span>, which is a new narrowed range.
| |
| − | | |
| − | Right Analysis:
| |
| − | got an R, lost an O. (otherwise, there would be no pheno change with R-->R)
| |
| − | was gene 13 or 14.
| |
| − | range is 12/26 to 14/26 or 46.153% to 53.846%
| |
| − | Combining with old range, new range is <span style="color:red;"> 44.153% to 50.000%</span>.
| |
| − | *could narrow this further w/ solvent testing.
| |
| − | </div>
| |
| − | | |
| − | New range for left splint: <span style="color:red;">13.636% to 15.384%</span>
| |
| − | See Test 3 under section for getting 2 cyan Inner stamen for details
| |
| − | | |
| − | ===Tay 13===
| |
| − | Calico, Slimy, Smelly Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 63/Ava 64)
| |
| − | Observed:
| |
| − | :Left Spoint: no seeds!
| |
| − | :Right Splint: no change in phenotype
| |
| − | | |
| − | Left splint was affected in (RO) gene, since it gave 0 seeds.
| |
| − | Left splint had a junk gene swapped, to either a R or an O.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *YORR
| |
| − | *YGYG
| |
| − | *ORYY
| |
| − | *ROYY
| |
| − | *YORY
| |
| − | *YYOG <-- that pinpoints it as 3rd Junk R gene, being replaced with an O.
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YY<span style="border:1px solid green;">O</span>G(RO)YY(OR)RK
| |
| − | :gene targeted was 19/27.
| |
| − | :gene range is 18/27 to 19/27 or <span style="color:red;">66.666% to 70.370%</span>
| |
| − | | |
| − | :The above pinpoints that the O gene in the (RO) gene set was replaced with an R.
| |
| − | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRGR<span style="border:1px solid green;">R</span>YY(OR)RK
| |
| − | :gene targeted was 22/27
| |
| − | :gene range is 21/27 to 22/27 or <span style="color:red;">77.777% to 81.481%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Vampire/Vampire (Bessodia's Test)
| |
| − | | |
| − | Left: KIYIYIOIYIIIOK
| |
| − | 10th gene switched
| |
| − | range is 9/12 to 10/12 or 75.000% to 83.333%
| |
| − | | |
| − | Right: KIYIYIOIYOOIOK
| |
| − | 9th gene switched
| |
| − | range is 8/12 to 9/12 or 66.666% to 75.000%
| |
| − | :both are consistent w/ above test.
| |
| − | </div>
| |
| − | | |
| − | ===Tay 14===
| |
| − | Fuzzy, Reticulated, Spotted, Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 65/Ava 66)
| |
| − | Observed:
| |
| − | :Left Spoint: 1 extra weed
| |
| − | :Right Splint: 1 extra seed
| |
| − | | |
| − | This means a new (RO) gene was created in the right gene, while a (OR) gene was broken in the left gene.
| |
| − | | |
| − | Solvents on Left Splint (Ava 65) showed:
| |
| − | *ROR (narrows it down to one of the end Y genes. Specifically, RGR could not become ROR, or there would be a new (OR) gene made as well, and it would be 0 weeds.)
| |
| − | *YGR
| |
| − | *YYR
| |
| − | *YOR
| |
| − | *KOR
| |
| − | *GRO
| |
| − | *YROR <-- that pinpoints it as the last Y in the genome being replaced with a R.
| |
| − | :gene targeted was 24/27.
| |
| − | :gene range is 23/27 to 24/27 or <span style="color:red;">85.185% to 88.888%</span>
| |
| − | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)Y<span style="border:1px solid green;">R</span>(OR)RK
| |
| − | | |
| − | Right Splint will have an R in (OR) gene replaced with a Y gene.
| |
| − | Solvents on Right Splint showed:
| |
| − | *RGY
| |
| − | *YORY
| |
| − | *YYOY <--- proves it to be the 2nd last R in genome.
| |
| − | :gene targeted is 25/27.
| |
| − | :gene range is 24/27 to 25/27 or <span style="color:red;">88.888% to 92.592%</span>
| |
| − | Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YYO<span style="border:1px solid green;">Y</span>RK
| |
| − | | |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Vampire/Vampire (Bessodia's Test)
| |
| − | | |
| − | Left: KIYIYIOIYIOIIK
| |
| − | 12th gene switched
| |
| − | range is 11/12 to 12/12 or 91.666% to 100%
| |
| − | | |
| − | R: KIYIYIOIYIOOOK
| |
| − | 11th gene is switched
| |
| − | range is 10/12 to 11/12 or 83.333% to 91.666%
| |
| − | | |
| − | *I suspect these splints were accidentally switched...
| |
| − | if so, the right range would now be <span style="color:red;">91.666% to 92.592%</span>
| |
| − | | |
| − | </div>
| |
| − | | |
| − | ===Tay 15===
| |
| − | Fuzzy Green Mottled Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 86/Ava 87)
| |
| − | Observed:
| |
| − | :Left Spoint: no water and no flax!
| |
| − | :Right Splint: 1 water, 2 weeds. (one extra weed step)
| |
| − | | |
| − | Left splint had (GYGYGY) gene groups broken in the middle GY
| |
| − | Left splint had an(OR) gene group broken.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *ORGR
| |
| − | *KORG
| |
| − | *GYRY <-- that pinpoints it as (GYGYGY) --> (GYRYGY)
| |
| − | Left Genome: K(OR)GR(GY<span style="border:1px solid green;">R</span>YGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 7/27.
| |
| − | :gene range is 6/27 to 7/27 or <span style="color:red;">22.222% to 25.925%</span>
| |
| − | :also reveals that a "G" gene was moved to the right splint
| |
| − | | |
| − | :Right Splint:
| |
| − | :The phenotype revealed that the (OR) -1 weed gene was mutated.
| |
| − | :The right gene results show that an "R" gene was switched w/ a "G" gene. So we are looking for (OR) -->OG
| |
| − | Solvents on Right showed:
| |
| − | *YYRG
| |
| − | *GROY
| |
| − | *RGRO
| |
| − | *GYOR
| |
| − | *OGGR <-- pinpoints as the first (OR) changed.
| |
| − | Right Genome: KO<span style="border:1px solid green;">G</span>GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 2/27
| |
| − | :gene range is 1/27 to 2/27 or <span style="color:red;">3.703% to 7.407%</span>
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Vampire/Vampire (Bessodia's Test)
| |
| − | | |
| − | Left: KIYIIIOIYIOIOK
| |
| − | :4th gene switched
| |
| − | :range is 3/12 to 4/12 or 25.000% to 33/333%
| |
| − | :narrows the range to <span style="color:red;">25.000% to 25.925%</span>
| |
| − | | |
| − | Right: KYYIYIOIYIOIOK
| |
| − | :1st gene switched
| |
| − | :range is 0/12 to 1/12 or 0% to 8.333%
| |
| − | :consistent w/ above range, but doesn't narrow it.
| |
| − | </div>
| |
| − | | |
| − | ===Tay 16===
| |
| − | Calico, Fuzzy, Prickly Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 148/Ava 149)
| |
| − | Observed:
| |
| − | :Left Spoint: no water, 2 weed, no flax
| |
| − | :Right Splint: normal
| |
| − | | |
| − | Left splint had the center G or Y in (GYGYGY) broken
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *RGYR <-- that pinpoints it as the middle G in(GYGYGY) as being replaced with an R
| |
| − | | |
| − | Left Genome: K(OR)GR(GY<span style="border:1px solid green;">R</span>YGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 7/27.
| |
| − | :gene range is 6/27 to 7/27 or <span style="color:red;">22.222% to 25.925%</span>
| |
| − | :also reveals that a "G" gene was moved to the left splint.
| |
| − | | |
| − | Right Splint had an R in junk genes targeted
| |
| − | solvents on Right splint showed:
| |
| − | *GROY
| |
| − | *YYOR
| |
| − | *YYGG <-- pinpoints 19th gene R --> G
| |
| − | Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YY<span style="border:1px solid green;">G</span>G(RO)YY(OR)RK
| |
| − | :gene targeted was 19/27
| |
| − | :gene range is 18/27 to 19/27 or <span style="color:red;">66.666% to 70.370%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2 Crown/Energy (Ava 198, Ava 199)
| |
| − | | |
| − | Expect: Crown to target gene 9 or 10 (U,R in UROO) and energy to target gene 29 or 30 (O of GRRO and U of UROR)
| |
| − | Observed: Crown lost Mw and gained Me so UROO --> UOOO
| |
| − | :Energy lost Yn and gained Cs (GRRO --> GRRR)
| |
| − | | |
| − | :Left splint: gene 10 of 37 was targeted
| |
| − | :range is 9/37 to 10/37 or 24.324% to 27.027%
| |
| − | :new left range is <span style="color:red;">24.324% to 25.925%</span>
| |
| − | | |
| − | :Right splint: gene 29 of 42 was targeted.
| |
| − | :range is 28/42 to 29/42 or 66.666% to 69.047%
| |
| − | :new right range is <span style="color:red;">66.666% to 769.047%</span>
| |
| − | </div>
| |
| − | | |
| − | ===Tay 17===
| |
| − | Calico, Ret, Slimy Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 150/Ava 151)
| |
| − | Observed:
| |
| − | :Left Splint: 2 weed, 1 water
| |
| − | :Right Splint: normal
| |
| − | | |
| − | Left splint had one of the three (OR) genes broken
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *YORR
| |
| − | *RYYR
| |
| − | *GYGR
| |
| − | *YYRG
| |
| − | *KORG
| |
| − | *RGYG
| |
| − | the only (OR) gene not coveredis the O in the second genes set
| |
| − | :gene targeted was 15/27
| |
| − | :gene range is 14/27 to 15/27 or <span style="color:red;">51.851% to 55.555%</span>
| |
| − | :also reveals that a O was moved to the Right splint.
| |
| − | | |
| − | Right Splint had junk gene targeted
| |
| − | solvents on Right splint showed:
| |
| − | *YGYG
| |
| − | *OYYO
| |
| − | *ROYY
| |
| − | *RYYR
| |
| − | *GROY
| |
| − | *GOOR <-- pinpoints 14th gene Y --> O
| |
| − | Right Genome: K(OR)GR(GYGYGY)GRG<span style="border:1px solid green;">O</span>(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 14/27
| |
| − | :gene range is 13/27 to 14/27 or <span style="color:red;">48.148% to 51.851%</span>
| |
| − | :Left genome: K(OR)GR(GYGYGY)GRGY(<span style="border:1px solid green;">Y</span>R)YYRG(RO)YY(OR)RK
| |
| − | </div>
| |
| − | | |
| − | ===Tay 18===
| |
| − | Fuzzy, Mottled Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 152/Ava 153)
| |
| − | Observed:
| |
| − | :Left Spoint: 0 seeds
| |
| − | :Right Splint: normal
| |
| − | | |
| − | Left splint had (RO) broken, Right splint had junk swapped.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *ORYY
| |
| − | *RRRO pinpoints it as the G right before the (RO) gene, being swapped to an R.
| |
| − | :Right genome: K(OR)GR(GYGYGY)GRGY(OR)YYR<span style="border:1px solid green;">R</span>(RO)YY(OR)RK
| |
| − | :gene targeted was 20/27
| |
| − | :gene range is 19/27 to 20/27 or <span style="color:red;">70.370% to 74.074%</span>
| |
| − | | |
| − | Left Splint had R in (RO) gene swapped to a G.
| |
| − | :Left genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG<span style="border:1px solid green;">R</span>OYY(OR)RK
| |
| − | :gene targeted was 21/27
| |
| − | :gene range is 20/27 to 21/27 or <span style="color:red;">74.074% to 77.777%</span>
| |
| − | | |
| − | </div>
| |
| − | | |
| − | ===Tay 19===
| |
| − | Green, Mottled, Spotted Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 156/Ava 157)
| |
| − | Observed:
| |
| − | :Left Spoint: no water, 1 flax (first, second, fifth or sixth gene in (GYGYGY) sequence broken)
| |
| − | :Right Splint: 3 weed (OR) broken
| |
| − | | |
| − | Solvent on Left:
| |
| − | *GROY
| |
| − | *OYYO
| |
| − | *RYYR
| |
| − | *ORYY
| |
| − | *RGRG
| |
| − | *RGYO
| |
| − | *YORY
| |
| − | *YYGR
| |
| − | *GRGO
| |
| − | *GYOR
| |
| − | *GRGR either 1st or 3rd Y in (GYGYGY) replaced with R. Can't tell, the symmetry of the genome is big. Would need a very large solvent.
| |
| − | So either 6th or 10th gene was targeted.
| |
| − | | |
| − | (determined from TEST 2 that it was 10th gene, see below for more details)
| |
| − | :10th gene of 27
| |
| − | :genome is K(OR)GR(GYGYG<span style="border:1px solid green;">R</span>)GRGY(OR)YYRG(OR)YY(OR)RK
| |
| − | :gene range is 9/27 to 10/27 or <span style="color:red;">33.333% to 37.037</span>
| |
| − | | |
| − | Right Splint:
| |
| − | *YGYG
| |
| − | *GRGY
| |
| − | *OYRK <-- pinpoints as the 26th gene, R --> Y
| |
| − | :Left genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(O<span style="border:1px solid green;">Y</span>)RK
| |
| − | :gene targeted is 26/27
| |
| − | :range is 25/27 to 26/27 or <span style="color:red;">92.592% to 96.296%</span>
| |
| − | </div>
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Energy / Silken
| |
| − | | |
| − | Silken should donate an I.
| |
| − | This will test to see if it was gene 6 or gene 10 in Test 1 that was swapped.
| |
| − | :if it was gene 6, then gene 8,9,10 or 11 will swap out of Energy (UYYY), inner East Yellow gene set
| |
| − | :if it was gene 10, then gene 14, 15, 16, or 17 will swap out of Energy (GYYY), Outer south yellow gene set
| |
| − | | |
| − | Mutagen Applied: Ava 158/Ava 159
| |
| − | Observed:
| |
| − | :RIght has IY gene set now from color observations. therefore, it was either 17th or 18th gene of 18 in Silken that swapped.
| |
| − | Can't tell more w/o solvent testing.
| |
| − | | |
| − | :Left has IY gene added and lost Yellow on south petal.
| |
| − | :therefore, it was gene 10 that was swapped in test 1. That test information has been updated.
| |
| − | :furthermore, because the IY is present, we know (GYYY) --> GYIY.
| |
| − | :here, gene 16 of 42 was swapped.
| |
| − | :genome is VVVVV UYYY UYYY GY<span style="border:1px solid green;">I</span>Y GYYY GRRO GRRO UROR UROR VVVVV
| |
| − | :gene range is 15/42 to 16/42 or 35.714% to 38.095%
| |
| − | :new Left range is <span style="color:red;">35.714% to 37.037</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: Vampire/vampire (bessodia's test)
| |
| − | | |
| − | Left: hit gene 5.
| |
| − | :range is 4/27 to 5/27 or 33.333% to 41.666%
| |
| − | :this is consistent w/ previous range
| |
| − | | |
| − | Right: hit gene 12
| |
| − | :range is 11/12 to 12/12 or 91.667% to 100%
| |
| − | :this is consistent w/ previous range
| |
| − | | |
| − | </div>
| |
| − | | |
| − | ==Sterope Recipes==
| |
| − | | |
| − | ===Sterope 02===
| |
| − | Crackly, Reticulated, Slimy Aryballa opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Blush/Crown (gives Ava 168, Ava 169)
| |
| − | | |
| − | Expected: Left to hit gene 16 or 17, Right to hit gene 2 or 3.
| |
| − | :if 16/2, blush loses IO, crown gains IOO and breaks Mw
| |
| − | :if 16/3 blush breaks IO, crown gains IO, breaks Mw
| |
| − | :if 17/2, blush breaks IO, crown breaks Mw
| |
| − | :if 17,3, O swaps with O, no changes.
| |
| − | | |
| − | Observed:
| |
| − | :no changes!!
| |
| − | :Therefore, left splint hit gene 17/26
| |
| − | :range is 16/26 to 17/26 or 61.538% to 65.384%
| |
| − | :new range is <span style="color:red;">63.334% to 65.384%</span>
| |
| − | :Right target was 3/37
| |
| − | :right range is 2/37 to 3/37, or 5.405% to 8.108%.
| |
| − | :this is consistent w/ previous tests.
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Dusk/Ava 26
| |
| − | should swap the 1st R from Ava 26. (if not, next Gene is an R too)
| |
| − | | |
| − | for Left splint, 4 possibilities:
| |
| − | | |
| − | 47th gene: GROR --> GRRR north magenta broken, South Cyan gained
| |
| − | 48th gene: GROR --> GROR no changes, same gene swapped (R)
| |
| − | 49th gene: IOOO --> ROOO broke magenta on outer, inner and middle stamen
| |
| − | 50th gene: IOOO --> IROO broken magenta on outer, inner and middle stament, gained cyan on outer stamen
| |
| − | | |
| − | Observed:
| |
| − | :Left splint (Ava 99) looks exactly like Dusk
| |
| − | :Right splint (Ava 100) is all white.
| |
| − | | |
| − | Therefore, the 48th gene was swapped from Left splint.
| |
| − | :48/73 gene
| |
| − | :range is now 47/73 to 48/73 or <span style="color:red;">64.383% to 65.753%</span>
| |
| − | </div>
| |
| − | | |
| − | ===Sterope 04===
| |
| − | Crackly, Dry Reticulated Sabahl opened
| |
| − | | |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 88/Ava 89)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *ORYY
| |
| − | *GRGY
| |
| − | *YORY
| |
| − | *ORRK
| |
| − | *RGYO
| |
| − | *OYYO
| |
| − | *RGRO
| |
| − | *RYYR
| |
| − | *RGYG
| |
| − | *GROY
| |
| − | no anomolies found yet. Standard Junk genes 4/27 and 11/27 not covered conclusively.
| |
| − | | |
| − | :Solvents on Right Splint showed:
| |
| − | *RGYG
| |
| − | *ORRK
| |
| − | *RGYO
| |
| − | *YRGR
| |
| − | | |
| − | | |
| − | no anomolies found yet. Starting to think this mutagen swapped same genes.
| |
| − | :this test is inconclusive (could go back to solvents more)
| |
| − | </div>
| |
| − | | |
| − | ===Sterope 05===
| |
| − | Phosphorescent, Prickly, Slimy Sabahl opened
| |
| − | | |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 118/Ava 120)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *YYOR
| |
| − | *YGRG
| |
| − | *RGYG
| |
| − | *GYOR
| |
| − | *YORR
| |
| − | *YORY
| |
| − | *RGYO
| |
| − | *KORG
| |
| − | *RGRG
| |
| − | *RGRO
| |
| − | *ORGR
| |
| − | *GRGY
| |
| − | *YGYG
| |
| − | *YYOR
| |
| − | *OYYO
| |
| − | no anomolies found yet. Standard Junk genes 18/27 not covered conclusively.
| |
| − | Seems to be that the same genes were switched.
| |
| − | </div>
| |
| − | | |
| − | ===Sterope 06===
| |
| − | Calico, Crackly, Spongy Avanya opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 117, Ava 118)
| |
| − | Observed:
| |
| − | :Left Spoint: 1 more weeding
| |
| − | :Right Splint: 2 seeds
| |
| − | | |
| − | Left splint had lost an (OR) gene
| |
| − | Right splint had gained (RO) gene
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *GYGY
| |
| − | *RGYY <-- that pinpoints it as the second (OR) --> YR
| |
| − | | |
| − | Left Genome: K(OR)GR(GYGYGY)GRGY<span style="border:1px solid green;">Y</span>RYYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 15/27.
| |
| − | :gene range is 14/27 to 15/27 or <span style="color:red;">51.851% to 55.555%</span>
| |
| − | :also reveals that a "O" gene was moved to the left splint
| |
| − | | |
| − | Solvents on Right Splint:
| |
| − | *ORGR
| |
| − | *YORR
| |
| − | *RGYO
| |
| − | *GRGY
| |
| − | *ROYR --> points to the 17th gene Y --> O.
| |
| − | | |
| − | Right sGenome: K(OR)GR(GYGYGY)GRGY(OR)<span style="border:1px solid green;">O</span>YRG(RO)YY(OR)RK
| |
| − | :gene targeted was 17/27
| |
| − | :gene range is 16/27 to 17/27 or <span style="color:red;">59.259% to 62.962%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Ava 29/Ava 29 (Clarity, Clarity, really)
| |
| − | | |
| − | Left: will target gene 19, 20, or 21. (GROR)(GRRO) (thats ORG in middle)
| |
| − | Right: will target gene 22 or 23, both R's in (GRRO)
| |
| − | | |
| − | Observed:
| |
| − | Left Splint (Ava 144) gained Cyan South (GRRR) and lost magenta North.
| |
| − | :that means gene 19 was targeted.
| |
| − | :range is 18/36 to 19/36 or 50.000% to 52.777%
| |
| − | :combining with test 1 results, the new range is <span style="color:red;">51.851% to 52.777%</span>
| |
| − | | |
| − | | |
| − | Right Splint (Ava 145) gained an O gene. Dont know where w/o using a solvent.
| |
| − | </div>
| |
| − | | |
| − | ==Celano Recipes==
| |
| − | | |
| − | ===Celano 01===
| |
| − | Dry, Spongy, Spotted Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 90/Ava 91)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *RGYO
| |
| − | *GYGR
| |
| − | *YYRG
| |
| − | *GYOR
| |
| − | *KORG
| |
| − | *YORY
| |
| − | *YGYG
| |
| − | *GYGY
| |
| − | *ORGR
| |
| − | *GROY
| |
| − | *RYYR
| |
| − | *RORG
| |
| − | *YGRG
| |
| − | | |
| − | covered all of the genome except the last 4 genes. Not showing anything yet, so stopping to save solvents...
| |
| − | no conclusions drawn yet
| |
| − | </div>
| |
| − | | |
| − | ===Celano 02===
| |
| − | Green, Mottled, Prickly Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 92/Ava 93)
| |
| − | Observed:
| |
| − | :Left Spoint: normal
| |
| − | :Right Splint: 2 weed, 1 flax 1 water.
| |
| − | | |
| − | Left splint had no gene groups broken
| |
| − | Right splint had (GYGYGY) broken (but not middle GY)
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *RYYR
| |
| − | *YYYG <-- that pinpoints it as the third G directly in(GYGYGY) as being replaced with an Y
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGY<span style="border:1px solid green;">Y</span>Y)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 9/27.
| |
| − | :gene range is 8/27 to 9/27 or <span style="color:red;">29.629% to 33.333%</span>
| |
| − | :also reveals that a "G" gene was moved to the left splint
| |
| − | | |
| − | Solvents on Left Splint:
| |
| − | *GYGY
| |
| − | *GYGR
| |
| − | *RGYR --> points to the 17th gene Y-->G.
| |
| − | | |
| − | Left Genome: K(OR)GR(GYGYGY)GRGY(OR)<span style="border:1px solid green;">G</span>YRG(RO)YY(OR)RK
| |
| − | :gene targeted was 17/27
| |
| − | :gene range is 16/27 to 17/27 or <span style="color:red;">59.259% to 62.962%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Ava 24/ Dusk (actually Ava99, which is exactly Dusk genome)
| |
| − | | |
| − | gives Ava 135 / Ava 136
| |
| − | | |
| − | expect: Left splint will donate gene 11 or 12. both are G's.
| |
| − | Ava 24 genome: RRROOOYYGGGGUUUIII
| |
| − | | |
| − | Right Splint:
| |
| − | should be gene 23 to gene 26: (gene set IYYY)
| |
| − | if 23, IYYY --> GYYY lose yellow on entire stamen, gain S yellow
| |
| − | if 24, IYYY --> IGYY lose yellow on entire stamen
| |
| − | if 25, IYYY --> IYGY lose yellow on inner and middle stamen
| |
| − | if 36, IYYY --> IYYG lose yellow on inner stamen.
| |
| − | | |
| − | Observed: Ava 136 looks like Dusk but with yellow on South petals, and one less yellow shade on all stamen parts.
| |
| − | therefore, gene 23 of 73 was targeted!
| |
| − | genome: GRORGORRUYYYGYORIYGRRO<span style="border:1px solid green;">G</span>YYYGRROGORRGRORROYGIOGRORIOOOIYYIYURRRGRROGORRUOOO
| |
| − | range is 22/73 to 23/73, a new range is <span style="color:red;">30.136% to 31.506%</span>
| |
| − | | |
| − | Left splint needs solvent to determine which gene was switched.
| |
| − | </div>
| |
| − | | |
| − | ===Celano 03===
| |
| − | Calico, Reticulated, Smelly Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. Ava 160/Ava 161
| |
| − | Observed:
| |
| − | :Left: normal + 1 Rotten flax (made RRGY gene set)
| |
| − | :Right: no seeds (RO) was broken
| |
| − | | |
| − | Right Splint:
| |
| − | :since we made an RR in the other gene, we know the right splint donated an R.
| |
| − | :since we broke the only RO gene set, it was the 21st gene targeted.
| |
| − | :Right genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(<span style="border:1px solid green;">G</span>O)YY(OR)RK
| |
| − | :range is 20/27 to 21/27 or <span style="color:red;">74.074% to 77.777%</span>
| |
| − | :(the fact that it was a G gene swapping in is from Left Splint info)
| |
| − | | |
| − | Left Splint:
| |
| − | solvent showed:
| |
| − | *ORYY
| |
| − | *YGYG
| |
| − | *ORRK
| |
| − | *RRGY
| |
| − | *ROYY
| |
| − | *YRGR
| |
| − | *GRGY
| |
| − | *YGYR <-- pinpoints as the G right after the (GYGYGY) set that was switched to R
| |
| − | :Gene targeted was 11th of 27, G --> R
| |
| − | :genome is :Right genome: K(OR)GR(GYGYGY)<span style="border:1px solid green;">R</span>RGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :range is 10/27 to 11/27 or <span style="color:red;">37.037% to 40.740%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Crown / vampire (Ava 184, Ava 185)
| |
| − | | |
| − | :Expected gene 14, 15, 16 on crown (U, R, V) and gene 9, 10 on vampire (I,O)
| |
| − | :Observed: no visible change on cornw (but we were targeting junk genes)
| |
| − | :vampire broke mageneta, IO , as expected.
| |
| − | Cannot tell more w/o solvents.
| |
| − | </div>
| |
| − | | |
| − | ===Celano 04===
| |
| − | Dry, Phos, Prickly Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 164/Ava 165)
| |
| − | Observed:
| |
| − | :Left Spoint: normal
| |
| − | :Right Splint: normal
| |
| − | | |
| − | either in junk genes or same gene was swapped.
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *ORRK
| |
| − | *YYOR
| |
| − | *YGRG
| |
| − | *RGYO
| |
| − | *ORGR
| |
| − | *GRGY
| |
| − | *RYRO <-- that pinpoints it as the G right before the (RO) gene set
| |
| − | | |
| − | :Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYR<span style="border:1px solid green;">Y</span>(RO)YY(OR)RK
| |
| − | :gene targeted was 20/27.
| |
| − | :gene range is 19/27 to 20/27 or <span style="color:red;">70.370% to 74.074%</span>
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *RGRG
| |
| − | *ROYG <-- that pinpoints it as the second Y after the (RO) gene set
| |
| − | | |
| − | :RightGenome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)Y<span style="border:1px solid green;">G</span>(OR)RK
| |
| − | :gene targeted was 24/27.
| |
| − | :gene range is 23/27 to 24/27 or <span style="color:red;">85.185% to 88.888%</span>
| |
| − | </div>
| |
| − | | |
| − | ===Celano 05===
| |
| − | Calico, Phos, Spotted Renard opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 196/Ava 197)
| |
| − | Observed:
| |
| − | :Left Spoint: 2 weeds (broke an (OR)
| |
| − | :Right Splint: normal (had junk gene swapped)
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *GRGY
| |
| − | *GYGR
| |
| − | *RGRO
| |
| − | *YRGR
| |
| − | *RRGR <-- that pinpoints it as the first gene, O --> R
| |
| − | | |
| − | Left Genome: K<span style="border:1px solid green;">R</span>RGR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 1/27.
| |
| − | :gene range is 0/27 to 1/27 or <span style="color:red;">0% to 3.703%</span>
| |
| − | :also reveals that a "O" gene was moved to the right splint
| |
| − | | |
| − | Solvents on Right Splint:
| |
| − | *GRGY
| |
| − | *YGRG
| |
| − | *ROYY
| |
| − | *YGYG
| |
| − | *GYGY
| |
| − | *ORYY
| |
| − | *ORGR
| |
| − | *OYYO
| |
| − | *KORG
| |
| − | *YOGR --> points to the 19th gene R --> O.
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YY<span style="border:1px solid green;">O</span>G(RO)YY(OR)RK
| |
| − | :gene targeted was 19/27
| |
| − | :gene range is 18/27 to 19/27 or <span style="color:red;">66.666% to 70.370%</span>
| |
| − | </div>
| |
| − | | |
| − | ==Alcyone Recipes==
| |
| − | | |
| − | ===Alcyone 01===
| |
| − | Calico, Crackly, Pricky
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen. (Ava 105/Ava 106)
| |
| − | Observed:
| |
| − | :Left Spoint: had an extra weed step (OR) was broken
| |
| − | :Right Splint: no change, must have mutated junk
| |
| − | | |
| − | Solvents on Left Splint showed:
| |
| − | *GYGY
| |
| − | *GYGR
| |
| − | *GRGY
| |
| − | *YRGR
| |
| − | *OGYY <-- that pinpoints it as the 4th R being replaced with a G.
| |
| − | | |
| − | Left Genome: K(OR)GR(GYGYGY)GRGY(O<span style="border:1px solid green;">R</span>)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 16/27.
| |
| − | :gene range is 15/27 to 16/27 or <span style="color:red;">55.555% to 59.259%</span>
| |
| − | :also reveals that a "G" gene was moved to the left splint
| |
| − | | |
| − | :Righgt Splint:
| |
| − | :The right gene results show that an "G" gene was switched w/ a "R" gene, in the junk sections
| |
| − | Solvents showed:
| |
| − | *KORG
| |
| − | *YORY
| |
| − | *RGRG
| |
| − | *YGYG
| |
| − | *YYRG
| |
| − | *ORYY
| |
| − | *YORR
| |
| − | *GRGY
| |
| − | *RYYR
| |
| − | *YYOR
| |
| − | *ORRK
| |
| − | *YGRR <--- pinpoints as 6th G being replaced w/ an R.
| |
| − | | |
| − | Right Genome: K(OR)GR(GYGYGY)GR<span style="border:1px solid green;">R</span>Y(OR)YYRG(RO)YY(OR)RK
| |
| − | :gene targeted was 13/27
| |
| − | :gene range is 12/27 to 13/27 or <span style="color:red;">44.444% to 48.148%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Vampire / Vampire (bessodia's test)
| |
| − | left result is gene 7/12
| |
| − | :range is 6/12 to 7/12 or 50.000% to 58.333%
| |
| − | : that means the new left range is <span style="color:red;">55.555% to 58.333%</span>
| |
| − | right result is gene 6/12
| |
| − | :range is 5/12 to 6/12 or 41.666% to 50.000%
| |
| − | :that range is consistent w/ the above results.
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: blush/morning (Ava 172, ava 173)
| |
| − | | |
| − | Expected: blush should hit gene 15, 16 (VI), Morning should hit gene 20-22 (YUR)
| |
| − | Observed: blush lost IO, so gene 16 was targeted! morning lost Ywest and gained IR, so gene 21 was hit
| |
| − | | |
| − | :Left gene is 16/26
| |
| − | :left range is 15/26 to 16/26 or 57.6925 to 61.538%
| |
| − | :new left range is <span style="color:red;">57.692% to 59.259%</span>
| |
| − | :Right gene is 21/44
| |
| − | :Right range is 20/44 to 21/44 or, the new range of <span style="color:red;">45.454% to 47.727%</span>
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 4: crown/blush (Ava 178, Ava 179)
| |
| − | | |
| − | Expected: gene 22 or 23 on crown (V O) and gene 12 or 13 on blush (R O)
| |
| − | :Observed: crown gained MW: URVO --> UROO
| |
| − | :Observed: blush lost Mw
| |
| − | | |
| − | :Left: gene 22 was targeted!
| |
| − | :gene is 22/37
| |
| − | :range is 21/37 to 22/37 or 56.756% to 59.459%
| |
| − | :this is consistent
| |
| − | | |
| − | :Right: gene 13 of 26 was targeted.
| |
| − | :range is 12/26 to 13/26 or 46.153% to 50.000%
| |
| − | :the new right range is <span style="color:red;">46.153% to 47.727%</span>
| |
| − | </div>
| |
| − | | |
| − | ===AC1 02 ===
| |
| − | Green, Phosphorescent, Smelly Avanya opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 131/Ava 132)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | Solvents on Right Splint showed:
| |
| − | *YYOR
| |
| − | *RYYR
| |
| − | *GYGY
| |
| − | *YGRG
| |
| − | *GRGY
| |
| − | *YORR
| |
| − | no anomolies found yet. Standard Junk genes mostly covered
| |
| − | | |
| − | :this test is inconclusive. Could do further solvent testing, but at this point, no results.
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 2: Ava 29/Ava 29. (Ava 29 has 4 junk genes, otherwise, its just a clarity bulb)
| |
| − | Ava 26 genome: IRIRIOIOIYIYGORUGRORGRROGORRGRORGRRO
| |
| − | | |
| − | mutagen applied: gave Ava 142 / Ava 143
| |
| − | | |
| − | Right Splint: observed: north petal lost a cyan (now is 2 red shades), and outer stament gained 1 cyan (+IR)
| |
| − | :The GORR gene set was targeted (to break North cyan) and the first R was switched to an I, to give GOIR.
| |
| − | :Right Genome: IRIRIOIOIYIYGORU GROR GRRO GO<span style="border:1px solid green;">I</span>R GROR GRRO
| |
| − | :27th gene of 36 was targeted
| |
| − | :range is 26/36 to 27/36 or <span style="color:red;">72.222% to 75.000%</span>
| |
| − | | |
| − | Left Splint: observed outer stamen lost 1 yellow
| |
| − | an IY gene was targeted, and it lost the I.
| |
| − | :so IYIY became either RYIY or IYRY
| |
| − | :either 9th or 11th gene targetd
| |
| − | :range is 8/36 to 11/36 or <span style="color:red;">22.222% to 30.555%</span>
| |
| − | could narrow this down by testing w/ solvents
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 3: vampire/energy (gave Ava 166, Ava 167)
| |
| − | expect gene 3 or 4 of vampire, and gene 31 or 32 of energy
| |
| − | :if gene 3 and gene 31, energy loses yellow west and gains IO
| |
| − | :if gene 3 and gene 32, energy loses Yw and gains IR
| |
| − | :if gene 4 and gene 31, energy loses Yw Vampire gains IR
| |
| − | :if gene 4 and gene 32, energy loses Yw. Vampire gains IO
| |
| − | | |
| − | Observed: energy lost Yw. STamen has no color. Vampire got an extra magenta on outer stamen
| |
| − | :therefore, left splint hit gene 3
| |
| − | :range is 16.667% to 25.000%
| |
| − | :new range is <span style="color:red;">22.222% to 25.000%</span>
| |
| − | :right splint hit gene 32 out of 42
| |
| − | :range is 31/42 to 32/42 or 73.809% to 76.190%
| |
| − | :new range is <span style="color:red;">73.809% to 75.000%</span>
| |
| − | </div>
| |
| − | | |
| − | ===AC 03 ===
| |
| − | Hairy, Slimy Avanya opened
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | TEST 1: Nile Green / Nile Green
| |
| − | Unmutated Genome:
| |
| − | :K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK
| |
| − | ::1 weed/1 water/1 seed/2 flax/0 rotten
| |
| − | | |
| − | applied mutagen.
| |
| − | Observed:
| |
| − | :both new splints had same phenotypes. (Ava 208/Ava 209)
| |
| − | | |
| − | Both splints had junk genes swapped, or same type of gene swapped.
| |
| − | | |
| − | :Solvents on Left Splint showed:
| |
| − | *YYOR
| |
| − | *OYYO
| |
| − | *YYRG
| |
| − | *YGRG
| |
| − | *YGYG
| |
| − | *RGYO
| |
| − | *RYYR
| |
| − | *RGYG
| |
| − | *RGRG
| |
| − | *ORYY
| |
| − | *YORR
| |
| − | | |
| − | no anomolies found, and standard junk genes all covered.
| |
| − | :The same gene types were swapped between splints
| |
| − | :this test is inconclusive
| |
| − | </div>
| |
| − | | |
| − | ==Crosses for Lily Requirements==
| |
| − | | |
| − | ===Aiming for 75% Green petals ===
| |
| − | :Stragety: Add cyan to one set of inner petals on Morning, then break one of the two yellow genes for that same set. This will turn that inner set of petals green. The outer two sets of petals are already green, so that will be 3/4 green.
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | CROSS 1: Morning / Crown with calico,dry, spotted (Sterope 01)
| |
| − | Aiming to swap first genes of both splints.
| |
| − | Unmutated Morning:
| |
| − | : K(IRRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
| − | : Cyan1OSMSIS(IRRR)+Yel2OSMSIS(IYYY)+Yel2IEP(UYYY)+Yel2IWP(UROR)+Cyan1OSP(GRRR)+Yel1OSP(GYYY)+Cyan1ONP(GORR)+Yel1ONP(GRRO)
| |
| − | Unmutated Crown:
| |
| − | : K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K
| |
| − | : Red3=[Mag3IWP(UROO)+Ye3IWP(UROR)] + 5V
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| − | | |
| − | :This mutagen will Turn (IRRR) (cyan on stamen) into (URRR), cyan on Inner East Petal.
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| − | | |
| − | Applied mutagen. (Ava 45/Ava46)
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| − | Observed:
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| − | :Left Spoint(Ava 45): Inner east petals now greenish yellow. <span style="color:red;">the cyan add was a success!!</span>
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| − | :Genome of Ava 45: K(<span style="border:1px solid green;">U</span>RRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
| − | | |
| − | :Right Splint(Ava 46): Inner petals look orange, not red
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| − | :Genome of Ava 46: K<span style="border:1px solid green;">I</span>ROO(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K
| |
| − | Right splint had first gene broken, UROO (magenta inner petals) --> IROO upper stamen cyan 1.
| |
| − | inner petal set now 3 yellow shades + 2 magenta shades = red + red + yellow, or a dark orange
| |
| − | </div>
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| − | | |
| − | <div style="margin:30px">
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| − | CROSS 2: Ava45 / Blush with Fuzzy, Hairy, Spongy (Tay 06)
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| − | : aiming to break up a (UYYY) inner yellow gene on Ava 45
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| − | Mutation should swap gene 18/44 (Y) on Ava 45 with gene 10/26 (V) blush.
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| − | Unmutated Ava 45: K(URRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
| − | Unmutated Blush: K(GROR)V(GOOO)V(UROO)V(IO)V(IOO)V(IOOO)K
| |
| − | | |
| − | Ava 47 / 48 resulted.
| |
| − | Observed:
| |
| − | left splint (Ava47) does have predicted colors. Green on outside petal sets. one green and one yellow inner petal sets.
| |
| − | right splint (Ava48): colors appear to be blush. No observed change.
| |
| − | | |
| − | Hypothesized Ava 47 genome:
| |
| − | :K(URRR)(IYYY)(IYYY)(UYYY)UY<span style="border:1px solid green;">V</span>Y(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K
| |
| − | Hypothesized Ava 48 Genome:
| |
| − | : K(GROR)V(GOOO)<span style="border:1px solid green;">Y</span>(UROO)V(IO)V(IOO)V(IOOO)K
| |
| − | | |
| − | both are consistent with expected and observed results
| |
| − | </div>
| |
| − | | |
| − | ===Aiming for Cyan on Inner Stamen ===
| |
| − | Maia needs this flower for Xenobotany
| |
| − | | |
| − | <div style="margin:30px">
| |
| − | Test 1: morning/vampire (Ava 127, Ava 128)
| |
| − | Mutagen Sterope 03
| |
| − | | |
| − | Expected: should convert a "GRRR gene to IRRR", which would be the second IRRR gene in morning.
| |
| − | Vampire should lose one IY, since it is donating an I, turning it into GY.
| |
| − | | |
| − | Observed:
| |
| − | results are as expected.
| |
| − | Vampire has 3 magenta 2 yellow on outer stamen, instead of 3 mag, 3 yellow.
| |
| − | Right genome: <span style="border:1px solid green;">G</span>YIYIOIYIOIO
| |
| − | | |
| − | Morning lost some cyan on south petal, but inner stamen is now two shades of green.
| |
| − | Left genome: IRRR IYYY IYYY UYYY UYYY UROR UROR <span style="border:1px solid green;">I</span>RRR GYYY GORR GRRO
| |
| − | | |
| − | tried to donate to Univ., but they said its not acceptable.
| |
| − | That means probably can't have any OTHER inner stamen colors.
| |
| − | Next step is to remove the two yellow shades then
| |
| − | </div>
| |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | Test 2: vampire/Ava 127 Tay09 (Calico, Green Smelly)
| |
| − | | |
| − | Vampire should donate 6th gene, O.
| |
| − | Ava 127 will hit either gene 9, 10, 11 in IYYY
| |
| − | *if 9, I in IYYY will be targeted --> OYYY removing 1 yellow from entire stamen
| |
| − | *if 10, first Y in IYYY will be targeted --> IOYY removing 1 yellow from entire stamen and gaining magenta on outer stamen
| |
| − | *if 11, second Y in IYYY will be targeted --> IYOY removing 1 yellow from middle and inner stamen
| |
| − | | |
| − | Mutagen applied, giving Ava 154 / Ava 155
| |
| − | Observed: Left Splint, Ava 154 has gained yellow on outer stamen. It was a Y it gained.
| |
| − | genome is: IYIOI<span style="border:1px solid green;">Y</span>IYIOIO
| |
| − | | |
| − | Right Splint, Ava 155 lost yellow on entire stamen and gained magenta on outer stamen.
| |
| − | :Therefore, 10th gene was targeted.
| |
| − | :Genome is IRRR IYYY I<span style="border:1px solid green;">O</span>YY UYYY UYYY UROR UROR IRRR GYYY GORR GRRO
| |
| − | :range is new for RIght splint
| |
| − | :range 9/44 to 10/44 or <span style="color:red;">20.454 to 22.727%</span>
| |
| − | first yellow shade was removed successfully
| |
| − | </div>
| |
| − | | |
| − | | |
| − | | |
| − | <div style="margin:30px">
| |
| − | Test 3: Ava 144 / Blush
| |
| − | mutagen Tay 12 (Calico, Crackly, Prickly)
| |
| − | | |
| − | Blush will donate either gene 13 or 14, either is an O
| |
| − | Ava 155 will have gene 6, 7 or 8 targeted. The Y's in IYYY
| |
| − | *if gene 6, IYYY --> IOYY
| |
| − | *if gene 7, IYYY --> IYOY
| |
| − | *if gene 8, IYYY --> IYYO
| |
| − | | |
| − | Mutagen applied, giving Ava 162/Ava 163
| |
| − | | |
| − | Left splint lost yellow on inner and middle stamen. outer stamen unchanged.
| |
| − | :therefore, gene 7 was targeted.
| |
| − | :range is 6/44 to 7/44 or <span style="color:red;">13.636% to 15.909%</span>.
| |
| − | :new min range for this mutagen!
| |
| − | :Genome is IRRR IY<span style="border:1px solid green;">O</span>Y IOYY UYYY UYYY UROR UROR IRRR GYYY GORR GRRO
| |
| − | second yellow shade was removed successfully
| |
| − | </div>
| |
| − | University Accepted!!!!
| |
