User:Avanya

Greetings!

Originally from the Bastet server, I transfered to Tale 5 only a few months ago with some friends. (Now we form the Bastet Hounds Guild). My primary passion in Egypt is glassblowing and glassmaking. I also enjoy the Test of Pyrotechnics, Thought puzzles, and crossbreeding flowers. I am always open to helping people in any way that I can, so please chat me anytime. I reside in Alcyone, at 1748, 2478. It's a short jog north of the chariot. My thought puzzle and Art coordinates can be found in my /info.

Glass Services

I sell most glass products, but I do not use a trading point system. I prefer to work out a trade that is fair and considered what the buyer has available to trade. My products include:

• Thermometers
• Barometers (5k, 6k quality)
• Hookah bowls (will assemble into finished hookah with your 14 canvas)
• Chemistry Calibration kits
• Distillation Coils 7.5k quality
• Wine glasses (sorry 8k quality and down, I need more practice)
• Mirrors (with your 200 silver powder though)

I am also willing to sell sheet glass, glass jars, glass pipes, glass rods, glass knives, glass torches. Bulk orders are subject to approval :)

Buying

I am currently buying materials needed to continue my work with characterizing mutagens. This includes:

• Mushrooms (Sand Spore, Slaves Bread, Razor's Edge, Peasant Foot, and Salt Water Fungus).
• Cobra Skins (as many as possible)

I am also always happy to purchase

• Silk, Sea Lily Bulbs, and Dung.

Yes Dung. I love Dung. (Test of Pyro should be renamed Test of Tons-of-Dung)

Star Labs

I am hoping to help Egypt make better use of its Portable Star Labs. I see so many of them laying around unused, or used as decorations. They are meant to be used and returned to a university of Art (preferably Sterope). I have a Law Petition circulating to help us use them more effectively. Please remember they are used for opening new Star recipes only...they are NOT part of the Principle or Test of Pyrotechnics!! They are given out in a limited amount, so they are a precious resource. Please see http://www.atitd.org/wiki/tale5/Portable_Star_Lab if you have any questions, or chat me in game.

Mutagenics Research

I am trying to help characterize the mutagens that are being researched. Specially, I'm trying to determine which gene ranges are swapped from the left and right splints. I won't bore you here with genetics theory, it is all explained much more clearly elsewhere on the wiki. But I would like to make my data available to others that share this interest.

This is my first time doing this, so PLEASE chat me if you think I am doing something wrong. Right now, our guru's of genetics are not playing, so I don't have a good source to verify this with. I welcome all input!!

I am not sure how the mutagen range rounds.

For example, if a mutagen range is that it will swap a gene at 10%. Given a genome of length 16, 10% along is between gene 1 and 2. Does it pick the closer gene (in that case, gene 2) or is it a floor rounding function? (that would truncate the extra %, and round down to gene 1). Or does it pick randomly between the two genes? Knowing this is important! I am assuming here it picks the closer gene, but further tests might reveal the actual rounding method.

Tay 01

Dry, Prickly, Reticulated Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 41/Ava 42) Observed:

Left Spoint: 1 weed/1 water/1 seed/2 flax/1 rotten flax

Right Splint: 0 zeed!!

Right splint had no gene groups broken, but gained an additional gene sequence of RRGY Left splint had the (RO) gene group broken.

Solvents on Left Splint showed:

• ORR
• GYG
• YRR <-- that pinpoints it as the third G directly after (GYGYGY) as being replaced with an R

Right Genome: K(OR)GR(GYGYGY)RRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 11/27.

gene range is 10.5/27 to 11.5/27 or 38.889% to 42.593%

also reveals that a "G" gene was moved to the left splint

Left Splint:

The phenotype revealed that the (RO) seed gene was mutated.

The right gene results show that an "R" gene was switched w/ a "G" gene.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(GO)YY(OR)RK

gene targeted was 21/27

gene range is 20.5/27 to 21.5/27 or 75.926% to 79.630%

TEST 2: Fracture / Crown (Ava 43,44)

phenotypes: Fracture became giant, colors unchanged. Crown petals went from red to magenta

Left splint: Fracture: K(ROYG)(ROY(G)YOR)GOR(GOOO)K

Test 1 predicts the 1st Y in the "ROYGYOR" set will be swapped. if so, it should be a 1 giant + 1 dwarf = small size.

observed: its a giant. no color changes

To be a size 1 giant, the both 1 giant and 1 dwarf gene must have been disabled. This would be accomplished if the shared "G" were swapped. This is just outside the predicted range of TEST 1. Perhaps I am not rounding out enough?

Right splint: Crown: K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Test 1 predicts that gene 28 or 29 will swap. (O or 2nd R in the first (UROR) set. If so, a yellow gene will be broken.

observed: the petals are more magenta colored than red. This is consistent w/ above prediction. It could also be a gene before or after this one that moved, and give the same results, so the exact gene position isn't certain w/o solvent testing.

Hypothesized Ava 43 genome:

K(ROYG)ROY(RO)YORGOR(GOOO)K

gene 8/18 targeted. 7.5/18 to 8.5/18 range is 41.667% to 47.222% for left splint target

Range overlap is 41.667% to 42.593%.

TEST 3: Dusk / Fracture (Ava 72, 73)

phenotypes: no changes were observed !!

Expected Left: gene 30 or 31. If 30 (GRRO) --> (GRRR)removing Yellow north and adding cyan south If 31 (GORR) --> RORR, breaking Cyan North.

Expected Right--> 14th gene, R from GOR to swap in. GOR is junk, so no phenotype change is expected.

Results: Either its gene 29 from Dusk that moved, R --R so no genome changes resulted then left range is 28.5/73 to 29.5/73 or 39.041% to 40.411%

OR

gene 14 from Fracture did not swap. (if gene 15 did, and gene 31 on Dusk, it would also be no change in genome... G-->G) Solvents are needed to resolve this further.

Tay 02

Calico, Green, Striped Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 37/Ava 38)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• RGRO
• GRGY
• ORYY
• ROYY
• GROY
• GRG
• GYOR
• YORY
• YGYG

no anomolies found yet. Standard Junk genes all covered

Solvents on Left Splint showed:

• GRGY
• ORYY
• YGRG
• GROY
• RGRO
• ORGR
• GYGY
• YGYG
• GYOR
• YRG
• ORRK

no anomolies found, and standard junk genes all covered.

this test is inconclusive

TEST 2: Silken / Vampire (Ava 26, 27)

phenotypes: Silken bulb unchanged, Vampire had center go magenta (ie had an IO get broken)

Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK

Right splint: Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K

so far, no conclusions made

Tay 03

Calico, Mottled, slimy Renard opened

TEST 1: Nile Green / Nile Green (Ava 35, Ava 36) Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

Left Splint: 1 weed/1 water/1 seed/2 flax/0 rotten

Right Splint: 1 weed/01 water/1 seed / 2 flax/ 0 rotten

Both splints had junk genes swapped.

Solvents on Right Splint showed:

• GGYG <-- that pinpoints it as 12th gene, R being replaced with G

Right Genome: K(OR)GR(GYGYG)GG)GY(OR)YYRG(OR)YY(OR)RK

gene targeted was 12/27.

gene range is 11.5/27 to 12.5/27 or 42.593% to 46.296%

also reveals that a "R" gene was moved to the left splint

Left Splint:

Looking for where a junk G was replaced with a R gene, and no new genes were created. (Phenotype was not altered)

Solvents on Left Splint showed:

• ORGR
• GYGY
• YGYG
• YORY
• RYYR
• ROYY
• RGR
• GYOR
• RGYO
• YYO
• YGY
• RGYG
• YRR <--- confirms G --> R switch. Only two places it could be. 11th or 20th.
• RRRO <-- proves 20th gene was swapped to R.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRR(RO)YY(OR)RK

gene targeted was 20/27

gene range is 19.5/27 to 20.5/27 or 72.222% to 75.926%

TEST 2: Silken / Clarity (Ava 28, Ava 29)

phenotypes: Silken bulb unchanged, Clarity had a cyan gene broken.

Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK

left target 72.222%-75.962% is 13/18 gene --> 1st U.

Right splint: Clarity Genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GORR)GRORGRROK

right target 42.593%-46.296% is 16/36 gene --> 2nd R in the first cyan gene set (GORR)

resultant right genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GORU)GRORGRROK

we know now what left genome is, since it's U was replaced with an R from Clarity:

resultant left genome: KRRROOOYYYGGGRUUIIIK

These results are compatible with observations!! (not confirmed with solvents, just observation and TEST 1 results)

Tay 04

Cracky, Prickly, Spongy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 49/Ava 50)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• not tested yet

Solvents on Left Splint showed:

• KORG
• ORGR
• RYY
• YGY, GYG
• YORY
• GROY
• ORYY
• GYGY
• RGRO
• YYRG
• YRGR
• GYGR
• RGYO

no anomolies found, and standard junk genes all covered. Seems that the Same Gene type was swapped between splints :( further tests are needed to characterize this mutagen

Tay 05

Crackly, Dry, spongy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 39/Ava 40)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• not tested yet

Solvents on Left Splint showed:

• GYGR
• RYYR
• YORR
• GROY
• OYY
• YRG
• RGR
• GYOR
• RGYO
• GYG
• OYYO
• ROYY
• GRGY

no anomolies found, and standard junk genes all covered. Seems that the Same Gene type was swapped between splints :(

TEST 2: Blush / Morning (Ava 31, 32)

phenotypes: Both phenotypes were unchanged

Left splint: Blush Genome: KGRORVGOOOVUROOVIOVIOOVIOOOK

Right splint: Morning: KIRRRIYYYIYYYUYYYUYYYURORURORGRRRGYYYGORRGRROK

so far, no conclusions made

Tay 06

Fuzzy, Hairy, SPongy, Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 33/Ava 34) Observed:

Left Spoint: 1 weed/1 water/1 seed/2 flax/0 rotten

Right Splint: 2 weed/0 water/1 seed / 1 flax/ 0 rotten

Right splint was affected in (GYGYGY) gene, specifically NOT the middle GY genes. Left splint had a junk gene swapped.

Solvents on Right Splint showed:

• RYY
• RGYG
• GGRG <-- that pinpoints it as the third Y in (GYGYGY) being replaced with a G

Right Genome: K(OR)GR(GYGYGG)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 10/27.

gene range is 9.5/27 to 10.5/27 or 35.185% to 38.889%

also reveals that a "Y" gene was moved to the left splint

Solvents on Left Splint showed:

• KOR
• ORRK
• RGY
• YYOR
• ROOY
• ORYY
• GYY <--- proves it

Looking for where a junk G was replaced with a Y gene, and no new genes were created. (Phenotype was not altered)

Left Genome: K(OR)GR(GYGYGY)YRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 11/27

gene range is 10.5/27 to 11.5/27 or 38.889% to 42.593%

Tay 07

Crackly, Hairy, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 51/Ava 52) Observed:

Left Spoint: 2 weed/1 water/1 seed/2 flax/0 rotten had an extra weed!

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten not changed

Left splint was affected in (OR) gene. Right splint had a junk gene swapped.

Solvents on Left Splint (Ava51) showed:

• YYOY <-- that pinpoints it as the last (OR) broken. R was replaced w/ Y.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OY))RK

gene targeted was 26/27

gene range is 25.5/27 to 26.5/27 or 94.444% to 98.145%

Right splint had a junk Y replaced. Solvents on Right Splint showed:

• GRGY
• GROY
• GYGY
• RGRO
• RGRG
• RRK
• YRG
• YYO
• YOR
• ORG
• ORR <--- pinpoints it (after other solvents eliminated some possibly Y's).

The 17th Y gene is swapped out and was replaced with an R

Right genome: K(OR)GR(GYGYGY)GRGY(OR)RYRG(RO)YY(OR))RK

gene targeted was 17/27

gene range is 16.5/27 to 17.5/27 or 61.111% to 64.815%

TEST 2: Clarity / Morning (Ava 69, Ava 70)

Left: this will swap either the 34th or 35th gene in Clarity. Both are R genes.

.... GRROK.

Right: this will swap either the 27, 28 or 29 gene (of 44) in Morning.

original genome: ...(UROR)(GRRR)...

if 27th, (UROR) --> (URRR). This will remove Yellow Inner West and add Cyan Inner East. if 28th (UROR) ---> (UROR). No change in genome or phenotype if 29th (GRRR)--> RRRR. this removes cyan from the outer south, and adds nothing.

Observed: Yellow inner west was removed and cyan inner east was added. Right genome:KIRRRIYYYIYYYUYYYUYYY(UROR)(UR(OR)R)GRRRGYYYGORRGRROK

gene targeted was 27/44.

gene range is: 26.5/44 to 27.5/44 or 60.227% to 62.500%.

combined with TEST 1's results, Right splint range is now: 61.111% to 62.500%

Left: GRRO gene was indeed broken, removing a yellow and leaving, in total, 1 black and 1 blue color on the Outer North Petals. No further conclusions can be made about which R gene was swapped.

Tay 08

Crackly, Phosphorescent, Slimy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 53/Ava 54) Observed:

Left Spoint: 0 weed/1 water/1 seed/0 flax/0 rotten ... no weed and no flax are different

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step

Left splint was affected in ROY sequence (which is required to harvest flax, see flax genome theories for more info). However, it was not a *(RO) that was changed, or the seed count would have gone down. Furthermore, we need a new OR gene, to account for the 1 less weed step.

looks like the 2nd to last Y should be switched to a R. ... YYRG(RO)RY(OR)RK. This would add (OR) and removed the (ROY).

Solvents on Left Splint showed:

• GYG
• RGR
• ROR <-- that pinpoints it as the gene we suspected.

the 22nd gene, Y, is replaced with an R

gene range is 21.5/27 to 22.5/27 or 83.333% to 87.037%

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)RY(OR)RK

Right splint lost an (-1 weed) gene, so (OR) was broken somewhere in the genome. We know a R was replaced with a Y, from the left splint tests.

Solvents on Right Splint showed:

• ORY
• ROY
• GRGY
• GYGY
• YOR
• ROYY
• YYRG
• RGR
• OYYO
• YORY
• KOY <--- shows its the second gene, R, that is swapped with a Y from the other splint

gene targeted was 2/27.

gene range is 1.5/27 to 2.5/27 or 5.556% to 9.259%

Right Genome: K(OY)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

Tay 09

Calico, Green, Smelly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 55/Ava 56)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• GRG
• GRO
• GYGY
• YGYG
• ORYY
• ORGR
• YORY
• YYOR
• RGYO
• OYYO
• GYGR
• RYY
• YRGR
• ORRK

no anomolies found yet. Standard Junk genes all covered.

This mutation swapped same gene type between splints

this test is inconclusive

Tay 10

Crackly, Spotted, Striped , Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 57/Ava 58) Observed:

Left Spoint: 2 weed/0 water/1 seed/0 flax/0 rotten

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten

Left splint was affected in (GYGYGY) gene, specifically in the middle GY genes. This breaks up both Flax genes (GYGY) as well as the water gene (GYGYGY), which would account for our observations.

Solvents on Left Splint showed:

• YGRG <-- that pinpoints it as the middle Y in (GYGYGY) being replaced with a R

Left Genome: K(OR)GR(GYGRGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 8/27.

gene range is 7.5/26 to 8.5/27 or 27.778% to 31.481%

Right splint had a -1 weed gene broken (OR). From above, we know it was the R that was swapped with a Y from the Left splint. Just have to identify which of the three (OR) genes was broken. Solvents on Right Splint showed:

• RGRG
• RGY
• GYG
• YRGY
• RGY
• ORG
• YGY
• ORY
• RGY
• YOR
• YYR
• RRY
• GYO
• OYRB <--- pinpoints the (OR) gene at the end as switching to (OY).

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OY)RK

gene targeted was 26/27

gene range is 25.5/27 to 26.5/27 or 94.444% to 98.148%

Tay 11

Phosphoresecent, Slimy, Smelly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 59/Ava 60) Observed:

Left Spoint: same phenotype

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step

Right splint had an extra weeding step. This means an (OR) gene was broken up.

Solvents on Right Splint showed:

• YRRR <-- that pinpoints it as the last O gene --> R gene.

the 25nd gene, O, is replaced with an R

gene range is 24.5/27 to 25.5/27 or 90.741% to 94.444%

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(RR)RK

Left splint lost an R gene in the junk genes somewhere, which was replaced with an O gene. Solvents on Left Splint showed:

• YGRG
• OGRO <--- shows its the 19th gene, R, that is swapped with a G from the other splint.

gene targeted was 19/27.

gene range is 18.5/27 to 19.5/27 or 68.519% to 72.222%

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYOG(RO)YY(OR)RK

Tay 12

Calico, Crackly, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 61/Ava 62)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• ORYY
• GYGR
• ROYY
• ORGR
• YRGR
• YYOR
• RGYG
• RGRG
• GYGY
• GRGY
• GYO
• RGRO
• RRK

no anomolies found yet. Standard Junk genes all covered.

This mutation swapped same gene type between these splints.

This test is inconclusive

Tay 13

Calico, Slimy, Smelly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 63/Ava 64) Observed:

Left Spoint: no seeds!

Right Splint: no change in phenotype

Left splint was affected in (RO) gene, since it gave 0 seeds. Left splint had a junk gene swapped, to either a R or an O.

Solvents on Right Splint showed:

• YORR
• YGYG
• ORYY
• ROYY
• YORY
• YYOG <-- that pinpoints it as 3rd Junk R gene, being replaced with an O.

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYOG(RO)YY(OR)RK

gene targeted was 19/27.

gene range is 18.5/27 to 19.5/27 or 68.519% to 72.222%

The above pinpoints that the O gene in the (RO) gene set was replaced with an R.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRGRRYY(OR)RK

gene targeted was 22/27

gene range is 21.5/27 to 22.5/27 or 79.630% to 83.333%

Tay 14

Fuzzy, Reticulated, Spotted, Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 65/Ava 66) Observed:

Left Spoint: 1 extra weed

Right Splint: 1 extra seed

This means a new (RO) gene was created in the right gene, while a (OR) gene was broken in the left gene.

Solvents on Left Splint (Ava 65) showed:

• ROR (narrows it down to one of the end Y genes. Specifically, RGR could not become ROR, or there would be a new (OR) gene made as well, and it would be 0 weeds.)
• YGR
• YYR
• YOR
• KOR
• GRO
• YROR <-- that pinpoints it as the last Y in the genome being replaced with a R.

gene targeted was 24/27.

gene range is 23.5/27 to 24.5/27 or 87.037% to 90.741%

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YR(OR)RK

Right Splint will have an R in (OR) gene replaced with a Y gene. Solvents on Right Splint showed:

• RGY
• YORY
• YYOY <--- proves it to be the 2nd last R in genome.

gene targeted is 25/27.

gene range is 24.5/27 to 25.5/27 or 90.741% to 94.444%

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YYOYRK

Tay 15

Fuzzy Green Mottled Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 86/Ava 87) Observed:

Left Spoint: no water and no flax!

Right Splint: 1 water, 2 weeds. (one extra weed step)

Left splint had (GYGYGY) gene groups broken in the middle GY Left splint had an(OR) gene group broken.

Solvents on Left Splint showed:

• ORGR
• KORG
• GYRY <-- that pinpoints it as (GYGYGY) --> (GYRYGY)

Left Genome: K(OR)GR(GYRYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 7/27.

gene range is 6.5/27 to 7.5/27 or 24.074% to 27.777%

also reveals that a "G" gene was moved to the right splint

Right Splint:

The phenotype revealed that the (OR) -1 weed gene was mutated.

The right gene results show that an "R" gene was switched w/ a "G" gene. So we are looking for (OR) -->OG

Solvents on Right showed:

• YYRG
• GROY
• RGRO
• GYOR
• OGGR <-- pinpoints as the first (OR) changed.

Right Genome: KOGGR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 2/27

gene range is 1.5/27 to 2.5/27 or 5.556% to 9.259%

Sterope 03

Dry, Fuzzy, Simey Aryballa opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 67/Ava 68) Observed:

Left Spoint: 0 weed!! ... got a new (OR) gene

Right Splint: 2 weed !! ... broke a (OR) gene

Solvents on Left Splint (Ava 67) showed:

• ORR
• YGYG
• GYGY
• GYGR
• ORGR
• GRGY
• YORR
• ORYO <-- that pinpoints it as the 6th Y being replaced with an O.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YORG(RO)YY(OR)RK

gene targeted was 18/27

gene range is 17.5/27 to 18.5/27 or 64.815% to 68.519%

Solvents on Right Splint (Ava 68) showed:

• ORR
• KYR <--- proves it. First O gene is replaced with a Y.

Right Genome: KYRGR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted is 1/27

gene range is 0.5/27 to 1.5/27 or 1.852% to 5.556%

Sterope 04

Crackly, Dry Reticulated Sabahl opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 88/Ava 89)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• ORYY
• GRGY
• YORY
• ORRK
• RGYO
• OYYO
• RGRO
• RYYR
• RGYG
• GROY

no anomolies found yet. Standard Junk genes 4/27 and 11/27 not covered conclusively.

Solvents on Right Splint showed:

• RGYG
• ORRK
• RGYO
• YRGR

no anomolies found yet. Starting to think this mutagen swapped same genes.

this test is inconclusive (could go back to solvents more)

Celano 01

Dry, Spongy, Spotted Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 90/Ava 91)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• RGYO
• GYGR
• YYRG
• GYOR
• KORG
• YORY
• YGYG
• GYGY
• ORGR
• GROY
• RYYR
• RORG
• YGRG

covered all of the genome except the last 4 genes. Not showing anything yet, so stopping to save solvents... no conclusions drawn yet

Celano 02

Green, Mottled, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 92/Ava 93) Observed:

Left Spoint: normal

Right Splint: 2 weed, 1 flax 1 water.

Left splint had no gene groups broken Right splint had (GYGYGY) broken (but not middle GY)

Solvents on Right Splint showed:

• RYYR
• YYYG <-- that pinpoints it as the third G directly in(GYGYGY) as being replaced with an Y

Right Genome: K(OR)GR(GYGYYY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 9/27.

gene range is 9.5/27 to 10.5/27 or 31.481% to 35.185%

also reveals that a "G" gene was moved to the left splint

Solvents on Left Splint:

• GYGY
• GYGR
• RGYR --> pointsto the 17th gene G --> Y.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)GYRG(RO)YY(OR)RK

gene targeted was 17/27

gene range is 16.5/27 to 17.5/27 or 61.111% to 64.815%

Crosses for Lily Requirements

Aiming for 75% Green petals

Stragety: Add cyan to one set of inner petals on Morning, then break one of the two yellow genes for that same set. This will turn that inner set of petals green. The outer two sets of petals are already green, so that will be 3/4 green.

CROSS 1: Morning / Crown with calico,dry, spotted (Sterope 01) Aiming to swap first genes of both splints. Unmutated Morning:

K(IRRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Cyan1OSMSIS(IRRR)+Yel2OSMSIS(IYYY)+Yel2IEP(UYYY)+Yel2IWP(UROR)+Cyan1OSP(GRRR)+Yel1OSP(GYYY)+Cyan1ONP(GORR)+Yel1ONP(GRRO)

Unmutated Crown:

K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Red3=[Mag3IWP(UROO)+Ye3IWP(UROR)] + 5V

This mutagen will Turn (IRRR) (cyan on stamen) into (URRR), cyan on Inner East Petal.

Applied mutagen. (Ava 45/Ava46) Observed:

Left Spoint(Ava 45): Inner east petals now greenish yellow. the cyan add was a success!!

Genome of Ava 45: K(URRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Right Splint(Ava 46): Inner petals look orange, not red

Genome of Ava 46: KIROO(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Right splint had first gene broken, UROO (magenta inner petals) --> IROO (junk). inner petal set now 3 yellow shades + 2 magenta shades = red + red + yellow, or a dark orange

CROSS 2: Ava45 / Blush with Fuzzy, Hairy, Spongy (Tay 06)

aiming to break up a (UYYY) inner yellow gene on Ava 45

Mutation should swap gene 18/44 (Y) on Ava 45 with gene 10/26 (V) blush. Unmutated Ava 45: K(URRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K Unmutated Blush: K(GROR)V(GOOO)V(UROO)V(IO)V(IOO)V(IOOO)K

Ava 47 / 48 resulted. Observed: left splint (Ava47) does have predicted colors. Green on outside petal sets. one green and one yellow inner petal sets. right splint (Ava48): colors appear to be blush. No observed change.

Hypothesized Ava 47 genome:

K(URRR)(IYYY)(IYYY)(UYYY)UYVY(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Hypothesized Ava 48 Genome:

K(GROR)V(GOOO)Y(UROO)V(IO)V(IOO)V(IOOO)K

both are consistent with expected and observed results