User:Avanya

Greetings!

Originally from the Bastet server, I transfered to Tale 5 only a few months ago with some friends. (Now we form the Bastet Hounds Guild). My primary passion in Egypt is glassblowing and glassmaking. I also enjoy the Test of Pyrotechnics, Thought puzzles, and crossbreeding flowers. I am always open to helping people in any way that I can, so please chat me anytime. I reside in Alcyone, at 1748, 2478. It's a short jog north of the chariot. My thought puzzle and Art coordinates can be found in my /info.

Glass Services

I sell most glass products, but I do not use a trading point system. I prefer to work out a trade that is fair and considered what the buyer has available to trade. My products include:

• Thermometers
• Barometers (5k, 6k quality)
• Hookah bowls (will assemble into finished hookah with your 14 canvas)
• Chemistry Calibration kits
• Distillation Coils 7.5k quality
• Wine glasses (sorry 8k quality and down, I need more practice)
• Mirrors (with your 200 silver powder though)

I am also willing to sell sheet glass, glass jars, glass pipes, glass rods, glass knives, glass torches. Bulk orders are subject to approval :)

For questions about getting started making glass, please see my guide: http://www.atitd.org/wiki/tale5/User:Avanya/Glassmaking_guide

Buying

I am currently buying materials needed to continue my work with characterizing mutagens. This includes:

• Mushrooms (Sand Spore, Slaves Bread, Razor's Edge, Peasant Foot, and Salt Water Fungus).
• Cobra Skins (as many as possible)

I am also always happy to purchase

• Silk, Sea Lily Bulbs, and Dung.

Yes Dung. I love Dung. (Test of Pyro should be renamed Test of Tons-of-Dung)

Star Labs

I am hoping to help Egypt make better use of its Portable Star Labs. I see so many of them laying around unused, or used as decorations. They are meant to be used and returned to a university of Art (preferably Sterope). I have a Law Petition circulating to help us use them more effectively. Please remember they are used for opening new Star recipes only...they are NOT part of the Principle or Test of Pyrotechnics!! They are given out in a limited amount, so they are a precious resource. Please see http://www.atitd.org/wiki/tale5/Portable_Star_Lab if you have any questions, or chat me in game.

Mutagenics Research:

I am trying to help characterize the mutagens that are being researched. Specially, I'm trying to determine which gene ranges are swapped from the left and right splints. I won't bore you here with genetics theory, it is all explained much more clearly elsewhere on the wiki. But I would like to make my data available to others that share this interest.

This is my first time doing this, so PLEASE chat me if you think I am doing something wrong. Right now, our guru's of genetics are not playing, so I don't have a good source to verify this with. I welcome all input!!

I am not sure how the mutagen range rounds.

For example, if a mutagen range is that it will swap a gene at 10%. Given a genome of length 16, 10% along is between gene 1 and 2. Does it pick the closer gene (in that case, gene 2) or is it a floor rounding function? (that would truncate the extra %, and round down to gene 1). Or does it pick randomly between the two genes? Knowing this is important! I am assuming here it picks the closer gene, but further tests might reveal the actual rounding method.

Tayete Recipes

Tay 01

Dry, Prickly, Reticulated Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 41/Ava 42) Observed:

Left Spoint: 1 weed/1 water/1 seed/2 flax/1 rotten flax

Right Splint: 0 zeed!!

Right splint had no gene groups broken, but gained an additional gene sequence of RRGY Left splint had the (RO) gene group broken.

Solvents on Left Splint showed:

• ORR
• GYG
• YRR <-- that pinpoints it as the third G directly after (GYGYGY) as being replaced with an R

Right Genome: K(OR)GR(GYGYGY)RRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 11/27.

gene range is 10.5/27 to 11.5/27 or 38.889% to 42.593%

also reveals that a "G" gene was moved to the left splint

Left Splint:

The phenotype revealed that the (RO) seed gene was mutated.

The right gene results show that an "R" gene was switched w/ a "G" gene.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(GO)YY(OR)RK

gene targeted was 21/27

gene range is 20.5/27 to 21.5/27 or 75.926% to 79.630%

TEST 2: Fracture / Crown (Ava 43,44)

phenotypes: Fracture became giant, colors unchanged. Crown petals went from red to magenta

Left splint: Fracture: K(ROYG)(ROY(G)YOR)GOR(GOOO)K

Test 1 predicts the 1st Y in the "ROYGYOR" set will be swapped. if so, it should be a 1 giant + 1 dwarf = small size.

observed: its a giant. no color changes

To be a size 1 giant, the both 1 giant and 1 dwarf gene must have been disabled. This would be accomplished if the shared "G" were swapped. This is just outside the predicted range of TEST 1. Perhaps I am not rounding out enough?

Right splint: Crown: K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Test 1 predicts that gene 28 or 29 will swap. (O or 2nd R in the first (UROR) set. If so, a yellow gene will be broken.

observed: the petals are more magenta colored than red. This is consistent w/ above prediction. It could also be a gene before or after this one that moved, and give the same results, so the exact gene position isn't certain w/o solvent testing.

Hypothesized Ava 43 genome:

K(ROYG)ROY(RO)YORGOR(GOOO)K

gene 8/18 targeted. 7.5/18 to 8.5/18 range is 41.667% to 47.222% for left splint target

Range overlap is 41.667% to 42.593%.

TEST 3: Dusk / Fracture (Ava 72, 73)

phenotypes: no changes were observed !!

Expected Left: gene 30 or 31. If 30 (GRRO) --> (GRRR)removing Yellow north and adding cyan south If 31 (GORR) --> RORR, breaking Cyan North.

Expected Right--> 14th gene, R from GOR to swap in. GOR is junk, so no phenotype change is expected.

Results: Either its gene 29 from Dusk that moved, R --R so no genome changes resulted then left range is 28.5/73 to 29.5/73 or 39.041% to 40.411%

OR

gene 14 from Fracture did not swap. (if gene 15 did, and gene 31 on Dusk, it would also be no change in genome... G-->G) Solvents are needed to resolve this further.

TEST 4: Vampire / Vampire (Bessodia's Test)

Vampire genome is: KIYIYIOIYIOIOK 12 genes long Left splint:

• IYIOI
• YOOIY
• YIOIO
• YIYIO
• OOIYI
• IYOOY
• OIOK
• KIYIY
• IYIYO
• YIYOO

an I gene swapped for an O gene: KIYIYOOIYIOIOK 5th gene was targeted. range is 4.5/12 to 5.5/12 or 37.500% to 45.833% that's consistent w/ the other test results!

Right splint:

• IOIY
• IYIO
• YIII
• IYII
• IIIO
• IYIY
• IIOK
• KIYI
• IIIO
• OIYI

genome is: KIYIYIOIYIIIOK 10th gene was targeted. range is 9.5/12 to 10.5/12 or 79.167% to 87.500%. that narrows the range down, when combined w/ other tests so the range is now 79.165% to 79.630%

Tay 02

Calico, Green, Striped Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 37/Ava 38)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• RGRO
• GRGY
• ORYY
• ROYY
• GROY
• GRG
• GYOR
• YORY
• YGYG

no anomolies found yet. Standard Junk genes all covered

Solvents on Left Splint showed:

• GRGY
• ORYY
• YGRG
• GROY
• RGRO
• ORGR
• GYGY
• YGYG
• GYOR
• YRG
• ORRK

no anomolies found, and standard junk genes all covered.

this test is inconclusive

TEST 2: Silken / Vampire (Ava 26, 27)

phenotypes: Silken bulb unchanged, Vampire had center go magenta (ie had an IO get broken)

Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK

Right splint: Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K

Left Splint:

• IIIK
• RRRO
• UIII
• YYYG
• YYYY <-- pinpoints as the last O --> G

genome is KRRROOYYYYGGGUUUIIIK 6th out of 18 gene targeted range is 5.5/18 to 6.51/18 which is 30.556% to 36.111%

Right Splint: know from phenotype we lost an IY, know from above we had Y-->O. so we trade IY for IO.

• YIOI
• KIYI
• IOIO
• IOIYI <-- pinpoints as the second Y

genome is KIYIOIOIYIOIOK 4th gene out of 12 range is 3.5/12 to 4.5/12 which is 29.167% to 37.500%.

TEST 3: Vampire/ Vampire (Bessodia's Test) Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K

no visual different between the flowers. solvent testing showed no changes. presuming the same gene type swapped. No conclusions available.

Tay 03

Calico, Mottled, slimy Renard opened

TEST 1: Nile Green / Nile Green (Ava 35, Ava 36) Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

Left Splint: 1 weed/1 water/1 seed/2 flax/0 rotten

Right Splint: 1 weed/01 water/1 seed / 2 flax/ 0 rotten

Both splints had junk genes swapped.

Solvents on Right Splint showed:

• GGYG <-- that pinpoints it as 12th gene, R being replaced with G

Right Genome: K(OR)GR(GYGYG)GG)GY(OR)YYRG(OR)YY(OR)RK

gene targeted was 12/27.

gene range is 11.5/27 to 12.5/27 or 42.593% to 46.296%

also reveals that a "R" gene was moved to the left splint

Left Splint:

Looking for where a junk G was replaced with a R gene, and no new genes were created. (Phenotype was not altered)

Solvents on Left Splint showed:

• ORGR
• GYGY
• YGYG
• YORY
• RYYR
• ROYY
• RGR
• GYOR
• RGYO
• YYO
• YGY
• RGYG
• YRR <--- confirms G --> R switch. Only two places it could be. 11th or 20th.
• RRRO <-- proves 20th gene was swapped to R.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRR(RO)YY(OR)RK

gene targeted was 20/27

gene range is 19.5/27 to 20.5/27 or 72.222% to 75.926%

TEST 2: Silken / Clarity (Ava 28, Ava 29)

phenotypes: Silken bulb unchanged, Clarity had a cyan gene broken.

Left splint: Silken Genome: KRRROOOYYYGGGUUUIIIK

left target 72.222%-75.962% is 13/18 gene --> 1st U.

Right splint: Clarity Genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GORR)GRORGRROK

right target 42.593%-46.296% is 15 or 16/36 gene --> 1sr or 2nd R in the first cyan gene set (GORR)

Solvent:

• IRIR
• RIOI
• OIYI
• UGRO <-- pinpoints as the 2nd R in (GORR), and the 16th gene

16 gene out of 36 range is 15.5/36 to 16.5/36 or 43.056% to 45.833%

resultant right genome: KIRIRIOIOIYIY(GORR)GRORGRRO(GORU)GRORGRROK

we know now what left genome is, since it's U was replaced with an R from Clarity:

resultant left genome: KRRROOOYYYGGGRUUIIIK

These results are compatible with observations Left Splint Solvent: GGGR. That confirms it. 13th gene targeted. range is 12.5/18 - 13.5/18 = 69.444% to 75.000% Combining with Test 1 results, new Left range is 72.222% to 75.000%

TEST 3: Vampire/ Vampire (Bessodia's Test) Vampire: K(IY)(IY)(IO)(IY)(IO)(IO)K

Left Splint:

• YOOI
• IOIY
• IYIO
• IYOO
• YIYI
• IYIY
• OOIO
• OIYO
• YIOI
• IOIK
• KIYI

9th gene was swapped: : K(IY)(IY)(IO)(IY)OO(IO)K range is 8.5/12 to 9.5/12 = 70.833% to 79.167%. That is consistent w/ other tests.

Right Splint:

• IYII
• IIIY
• IOIK
• IIYI
• KIYI
• YIOI

6th gene was swapped: KIYIYIIIYIOIOK range is 45.833% to 54.167%. This overlaps exactly at 45.833%. exactly

TEST 4: Crown / Fracture MEANT to do crown / clarity....oops

yielded Ava 121, Ava 122

Left: Broke UROR (Yellow West). Not sure if gene 27 (R) or gene 28(O) w/o using solvents.

Right: (ROYG)(ROY_YOR)GOR(GOO) broke that middle _ gene (was a G). it broke up a giant and a dwarf gene, leaving just 1 giant gene in the genome. can't tell what was swapped in, w/o solvent.

test inconclusive, w/o solvents.

TEST 5: Crown/Clarity (Ava 129. Ava 130)

expected: Crown's gene 27 or 28 ... UROR... (the RO in the middle) expected: Clarity's genes 15, 16, 17. gene 15, 16 are R's in GORR gene 17 is G in GROR

Observed: phenotypes of flowers are the same!! so both splints swapped R's.

Left: gene 27 of 37 was targeted range is 26.5/37 to 27.5/37 or 71.612% to 74.324% combining with other test results, the new left splint range is: 72.222% to 74.324%.

Right Splint: was gene 15 or 16 of 36 total range is 14.5/36 to 16.5/36 or 40.278% to 45.833% This overlaps exactly at 45.833%.... again.

Tay 04

Cracky, Prickly, Spongy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 49/Ava 50)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• not tested yet

Solvents on Left Splint showed:

• KORG
• ORGR
• RYY
• YGY, GYG
• YORY
• GROY
• ORYY
• GYGY
• RGRO
• YYRG
• YRGR
• GYGR
• RGYO

no anomolies found, and standard junk genes all covered. Seems that the Same Gene type was swapped between splints :( further tests are needed to characterize this mutagen

TEST 2: Vampire / vampire (Bessodia's Test) Vampire: KIYIYIOIYIOIOK 12 genes

Right splint:

• IYIO
• YYIO
• YIOI
• KIYY
• IYYY
• IOIY
• OIYI
• IYYY
• 0IOK

3rd gene was swapped: KIYYYIOIYIOIOK range is 2.5/12 to 3.5/12 which is 20.833% to 29.167%

Left Splint:

• KIII

2nd gebe was swapped. KIIIYIOIYIOIOK range is 1.5/12 to 2.5/12 which is 12.500% to 20.833%

Tay 05

Crackly, Dry, spongy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 39/Ava 40)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• not tested yet

Solvents on Left Splint showed:

• GYGR
• RYYR
• YORR
• GROY
• OYY
• YRG
• RGR
• GYOR
• RGYO
• GYG
• OYYO
• ROYY
• GRGY

no anomolies found, and standard junk genes all covered. Seems that the Same Gene type was swapped between splints :(

TEST 2: Blush / Morning (Ava 31, 32)

phenotypes: Both phenotypes were unchanged

Left splint: Blush Genome: KGRORVGOOOVUROOVIOVIOOVIOOOK

Right splint: Morning: KIRRRIYYYIYYYUYYYUYYYURORURORGRRRGYYYGORRGRROK

so far, no conclusions made

TEST 3: Vampire / vampire (Bessodia's Test) Vampire: KIYIYIOIYIOIOK 12 genes

Right splint:

• IIOI
• IIIO
• IYIY
• IYIO
• IOIO
• YIYI
• IOII
• KIYI
• OIOK

8th gene was swapped: KIYIYIOIIIOIOK range is 7.5/12 to 8.5/12 which is 62.500% to 70.833%

Left Splint:

• YIYI
• IOYY
• YIOI
• OIOK
• IOYY
• KIYI
• OYYI

7th gene was swapped. KIYIYIOYYIOIOK range is 6.5/12 to 7.5/12 which is 54.167% to 62.500%

Tay 06

Fuzzy, Hairy, SPongy, Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 33/Ava 34) Observed:

Left Spoint: 1 weed/1 water/1 seed/2 flax/0 rotten

Right Splint: 2 weed/0 water/1 seed / 1 flax/ 0 rotten

Right splint was affected in (GYGYGY) gene, specifically NOT the middle GY genes. Left splint had a junk gene swapped.

Solvents on Right Splint showed:

• RYY
• RGYG
• GGRG <-- that pinpoints it as the third Y in (GYGYGY) being replaced with a G

Right Genome: K(OR)GR(GYGYGG)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 10/27.

gene range is 9.5/27 to 10.5/27 or 35.185% to 38.889%

also reveals that a "Y" gene was moved to the left splint

Solvents on Left Splint showed:

• KOR
• ORRK
• RGY
• YYOR
• ROOY
• ORYY
• GYY <--- proves it

Looking for where a junk G was replaced with a Y gene, and no new genes were created. (Phenotype was not altered)

Left Genome: K(OR)GR(GYGYGY)YRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 11/27

gene range is 10.5/27 to 11.5/27 or 38.889% to 42.593%

TEST 2: Ava 45 / Blush

This is Step #2 in section below, trying for 75% green flower. Left: (UYYY) yellow gene was broken on Ava 45. need to do solvent testing to know which gene it was exactly.

Right: predicted either gene 9 or 10 to swap.

 gene 9 would have broken (GOOO)
 Gene 0 is a V. no phenotype change expected.

No phenotype change of blush was seen. Therefore, we know 10th gene V swapped. 10/26 gene targeted range is 9.5/26 to 10.5/26 = 36.538% to 40.385% combining with test 1, that gives overlap range of 36.538% to 38.889%.

Tay 07

Crackly, Hairy, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 51/Ava 52) Observed:

Left Spoint: 2 weed/1 water/1 seed/2 flax/0 rotten had an extra weed!

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten not changed

Left splint was affected in (OR) gene. Right splint had a junk gene swapped.

Solvents on Left Splint (Ava51) showed:

• YYOY <-- that pinpoints it as the last (OR) broken. R was replaced w/ Y.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OY))RK

gene targeted was 26/27

gene range is 25.5/27 to 26.5/27 or 94.444% to 98.145%

Right splint had a junk Y replaced. Solvents on Right Splint showed:

• GRGY
• GROY
• GYGY
• RGRO
• RGRG
• RRK
• YRG
• YYO
• YOR
• ORG
• ORR <--- pinpoints it (after other solvents eliminated some possibly Y's).

The 17th Y gene is swapped out and was replaced with an R

Right genome: K(OR)GR(GYGYGY)GRGY(OR)RYRG(RO)YY(OR))RK

gene targeted was 17/27

gene range is 16.5/27 to 17.5/27 or 61.111% to 64.815%

TEST 2: Clarity / Morning (Ava 69, Ava 70)

Left: this will swap either the 34th or 35th gene in Clarity. Both are R genes.

.... GRROK.

Right: this will swap either the 27, 28 or 29 gene (of 44) in Morning.

original genome: ...(UROR)(GRRR)...

if 27th, (UROR) --> (URRR). This will remove Yellow Inner West and add Cyan Inner East. if 28th (UROR) ---> (UROR). No change in genome or phenotype if 29th (GRRR)--> RRRR. this removes cyan from the outer south, and adds nothing.

Observed: Yellow inner west was removed and cyan inner east was added. Right genome:KIRRRIYYYIYYYUYYYUYYY(UROR)(UR(OR)R)GRRRGYYYGORRGRROK

gene targeted was 27/44.

gene range is: 26.5/44 to 27.5/44 or 60.227% to 62.500%.

combined with TEST 1's results, Right splint range is now: 61.111% to 62.500%

Left: GRRO gene was indeed broken, removing a yellow and leaving, in total, 1 black and 1 blue color on the Outer North Petals. No further conclusions can be made about which R gene was swapped.

TEST 3: Vampire/vampire (Bessodia's Test) Left genome: IYIYIOIOIOIO. 8th gene 0-->Y range is 62.500% to 70.833% overlaps only at edge of range, 62.500%

Right genome: IYIYIOIYIOIY 12th gene Y-->O range is 95.833% - 100% updates range to 95.833% to 98.143%

TEST 4: Crown / Fracture (Ava 125, Ava 126)

expect: gene 35 or 36 in crown (center RO in UROR gene). Fracture just donates an R.

Observed: Left has 1 cyan east and lost 1 yellow west. so gene 36 was swapped .. UROR --> URRR gene 36/37 targeted genome: UROO UROO UROO V URVOOVURVORV UROR UROR URRR

range is 35.5/37 to 36.5/37 or 95.946% to 98.649% combining with other test results, new range is 95.946% to 98.143%

Right: colors are the same, but its a giant x 2!! we know it was an R being swapped out with an O. genome: ROYG ROYGYOO GOR GOOO gene 11/18 was targeted range is 10.5/18 to 11.5/18 or 58.333% to 63.889% This is wider than our current range.

Tay 08

Crackly, Phosphorescent, Slimy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 53/Ava 54) Observed:

Left Spoint: 0 weed/1 water/1 seed/0 flax/0 rotten ... no weed and no flax are different

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step

Left splint was affected in ROY sequence (which is required to harvest flax, see flax genome theories for more info). However, it was not a *(RO) that was changed, or the seed count would have gone down. Furthermore, we need a new OR gene, to account for the 1 less weed step.

looks like the 2nd to last Y should be switched to a R. ... YYRG(RO)RY(OR)RK. This would add (OR) and removed the (ROY).

Solvents on Left Splint showed:

• GYG
• RGR
• ROR <-- that pinpoints it as the gene we suspected.

the 22nd gene, Y, is replaced with an R

gene range is 21.5/27 to 22.5/27 or 83.333% to 87.037%

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)RY(OR)RK

Right splint lost an (-1 weed) gene, so (OR) was broken somewhere in the genome. We know a R was replaced with a Y, from the left splint tests.

Solvents on Right Splint showed:

• ORY
• ROY
• GRGY
• GYGY
• YOR
• ROYY
• YYRG
• RGR
• OYYO
• YORY
• KOY <--- shows its the second gene, R, that is swapped with a Y from the other splint

gene targeted was 2/27.

gene range is 1.5/27 to 2.5/27 or 5.556% to 9.259%

Right Genome: K(OY)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

TEST 2: Crown / Ava 28 (Ava 123, Ava 124)

Expected: Crown will have gene 31 or 32 targeted (center RO in UROR gene set) If 31, there will be no color change (R --> R) If 32, we will lose Ywest and gain Ceast (UROR --> URRR)

Ava 28 is just donating an R from position 1 or 2.

Observed: Left: Cyan East is present, and one Yellow west was lost. so Gene 32 was targeted. Genome: UROOUROOUROOVURVOOVURVORVUROR URRR UROR range is 31.5/37 to 32.5/37 or 85.135% to 87.037% combining with other test results, the range is now 85.135% to 87.037%

Right: original genome of Ava 28: KRRROOYYYYGGGUUUIIIK either gene 1 or 2 was swapped, so resulting genome is: KORROOYYYYGGGUUUIIIK OR KROROOYYYYGGGUUUIIIK. Can't tell which w/o solvent test. so this splint info is inconclusive.

Tay 09

Calico, Green, Smelly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 55/Ava 56)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• GRG
• GRO
• GYGY
• YGYG
• ORYY
• ORGR
• YORY
• YYOR
• RGYO
• OYYO
• GYGR
• RYY
• YRGR
• ORRK

no anomolies found yet. Standard Junk genes all covered.

This mutation swapped same gene type between splints

this test is inconclusive

TEST 2: Vampire/Vampire (Bessodia's Test)

R: KIYOYIOIYIOIOK 3rd gene was switched range is 2.5/12 to 3.5/12 or 20.833% to 29.157%

Left: KIYIYIIIYIOIOK 6th gene was switched range is 5.5/12 to 6.5/12 or 45.833% to 54.167%

TEST 3: Silken/Blush Ava 24/Ava25

observed: blush lost magenta south, silken still white

Right Splint (Ava 25) original genome is blush:GRORVGOOOVUROOVIOVIOOVIOOO expected target range is gene 6 to 9 (GOOO) Solvent:

• VURO
• VYOO
• OOVU
• OVIO
• OOVI
• ORVY

6th gene was target G --> Y correct genome is:GRORV Y OOOVUROOVIOVIOOVIOOO range is 5.5/26 to 6.5/26 or 21.154% to 25.000%, a new range

Left Splint (Ava 25) original genome is silken: RRROOOYYYGGGUUUIII we know it gave a Y to other splint and got a G Solvent:

• UUII
• UIII
• OOYY
• KRRR
• GGGG <-- pinpoints as the last Y being swapped for a G

gene is 9th of 18 range is 8.5/18 to 9.5/18 or 7.222% to 52.778%, a new range. correct genome is: RRROOOYYGGGGUUUIII

Tay 10

Crackly, Spotted, Striped , Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 57/Ava 58) Observed:

Left Spoint: 2 weed/0 water/1 seed/0 flax/0 rotten

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten

Left splint was affected in (GYGYGY) gene, specifically in the middle GY genes. This breaks up both Flax genes (GYGY) as well as the water gene (GYGYGY), which would account for our observations.

Solvents on Left Splint showed:

• YGRG <-- that pinpoints it as the middle Y in (GYGYGY) being replaced with a R

Left Genome: K(OR)GR(GYGRGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 8/27.

gene range is 7.5/26 to 8.5/27 or 27.778% to 31.481%

Right splint had a -1 weed gene broken (OR). From above, we know it was the R that was swapped with a Y from the Left splint. Just have to identify which of the three (OR) genes was broken. Solvents on Right Splint showed:

• RGRG
• RGY
• GYG
• YRGY
• RGY
• ORG
• YGY
• ORY
• RGY
• YOR
• YYR
• RRY
• GYO
• OYRB <--- pinpoints the (OR) gene at the end as switching to (OY).

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OY)RK

gene targeted was 26/27

gene range is 25.5/27 to 26.5/27 or 94.444% to 98.148%

TEST 2: morning / Ava 28 yields flowers Ava 103, Ava 104

Left: Above test 1 predicts gene 12-14 will be chosen, and that Ava 28 will donate a "I" gene morning genome: K IRRR IYYY IYYY UYYY ... if 12th gives ... IYYI UYYY... would lose 1 yellow on inner stamen if 13th, gives ... IYYY IYYY... would lose 1 yellow East, gain 1 yellow on entire stamen if 14th, gives ... IYYY UIYY... would lose 1 yellow East, gain 1 yellow on outer and middle stamen

Observed: top and middle staymen are same. Inner stamen is more blueish. East petals are the same. therefore, the 12th gene was exchanged. so the range is 11.5/44 to 12.5/44 or 26.778% to 28.409% combining that with Test 1 results, the new range is 27.778% to 28.409%

Right: Test 1 predicted that gene 17 or 18 would be switched. Ava 28 genome is: KRRROOOYYYGGGRUUIIIK Either is an I gene. if 17th, gives ...UUIYIK would introduce yellow on the top stamen if 18th, gives ...UUIIYK would still appear all white.

Observed: the top stamen has 1 shade of yellow now. therefore, the 17th gene was swapped. the range is 16.5/18 to 17.5/18 or 91.667% to 97.222% combining hat with Test 1 results, the new range is 94.444% to 97.222%

TEST 3: Vampire/Vampire (Bessodia's Test)

Left: KIYIOIOYIOIOK 4th gene swapped range is 3.5/12 to 4.5/12 or 29.167% to 37.500%

 Doesn't quite match tests above...

Right: KIYIYIOIYIOIK 12th gene is switched range is 95.833% to 100% combining with above test results... New range is 95.833% to 97.222%

Tay 11

Phosphoresecent, Slimy, Smelly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 59/Ava 60) Observed:

Left Spoint: same phenotype

Right Splint: 2 weed/1 water/1 seed / 2 flax/ 0 rotten... 1 extra weed step

Right splint had an extra weeding step. This means an (OR) gene was broken up.

Solvents on Right Splint showed:

• YRRR <-- that pinpoints it as the last O gene --> R gene.

the 25nd gene, O, is replaced with an R

gene range is 24.5/27 to 25.5/27 or 90.741% to 94.444%

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(RR)RK

Left splint lost an R gene in the junk genes somewhere, which was replaced with an O gene. Solvents on Left Splint showed:

• YGRG
• OGRO <--- shows its the 19th gene, R, that is swapped with a G from the other splint.

gene targeted was 19/27.

gene range is 18.5/27 to 19.5/27 or 68.519% to 72.222%

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYOG(RO)YY(OR)RK

Tay 12

Calico, Crackly, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 61/Ava 62)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• ORYY
• GYGR
• ROYY
• ORGR
• YRGR
• YYOR
• RGYG
• RGRG
• GYGY
• GRGY
• GYO
• RGRO
• RRK

no anomolies found yet. Standard Junk genes all covered.

This mutation swapped same gene type between these splints.

This test is inconclusive

TEST 2: Vampire/Vampire (Bessodia's Test)

Left: KIOIYIOIYIOIOK 2nd gene switched range is 1.5/12 to 2.5/12 or 13.500% to 20.833%

Right: KIYIYIYIYIOIOK 6th gene switched range is 5.5/12 to 6.5/12 or 45.833% to 54.167%

TEST 3: Ava 25/Ava 25

Ava 25 flower is blush with 1 mutated gene: GROR V YOOO V UROO V IO V IOO V IOOO Expected range: left gene 3-5 (O,R,V) , right gene 12-14 (R,O,O)

Ava 133 / Ava 134 result

Observed: Left: Magenta west, all other petals are white. therefore, GROR was broken Right: Magenta north, all other petals are white. Therefore, UROO was broken

Left Analysis: was not gene 5 (V) or there would have been no phenotype change. was not gene 3(O) or would be either GROR or GRRR, both are colors that are not present. it was gene 4 (R) of 26 total. range is 3.5/26 to 4.5/26 or 13.462% to 17.308%, which is a new narrowed range.

Right Analysis: got an R, lost an O. (otherwise, there would be no pheno change with R-->R) was gene 13 or 14. range is 12.5/26 to 14.5/26 or 48.077% to 55.769% Combining with old range, new range is 48.077% to 54.167%.

• could narrow this further w/ solvent testing.

Tay 13

Calico, Slimy, Smelly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 63/Ava 64) Observed:

Left Spoint: no seeds!

Right Splint: no change in phenotype

Left splint was affected in (RO) gene, since it gave 0 seeds. Left splint had a junk gene swapped, to either a R or an O.

Solvents on Right Splint showed:

• YORR
• YGYG
• ORYY
• ROYY
• YORY
• YYOG <-- that pinpoints it as 3rd Junk R gene, being replaced with an O.

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYOG(RO)YY(OR)RK

gene targeted was 19/27.

gene range is 18.5/27 to 19.5/27 or 68.519% to 72.222%

The above pinpoints that the O gene in the (RO) gene set was replaced with an R.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRGRRYY(OR)RK

gene targeted was 22/27

gene range is 21.5/27 to 22.5/27 or 79.630% to 83.333%

TEST 2: Vampire/Vampire (Bessodia's Test)

Left: KIYIYIOIYIIIOK 10th gene switched range is 9.5/12 yo 10.5/12 or 79.167% to 87.500%

Right: KIYIYIOIYOOIOK 9th gene switched range is 8.5/12 to 9.5/12 or 70.833% to 79.167% combining w/ Test 1 results narrows the range to 70.833% to 72.222%

Tay 14

Fuzzy, Reticulated, Spotted, Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 65/Ava 66) Observed:

Left Spoint: 1 extra weed

Right Splint: 1 extra seed

This means a new (RO) gene was created in the right gene, while a (OR) gene was broken in the left gene.

Solvents on Left Splint (Ava 65) showed:

• ROR (narrows it down to one of the end Y genes. Specifically, RGR could not become ROR, or there would be a new (OR) gene made as well, and it would be 0 weeds.)
• YGR
• YYR
• YOR
• KOR
• GRO
• YROR <-- that pinpoints it as the last Y in the genome being replaced with a R.

gene targeted was 24/27.

gene range is 23.5/27 to 24.5/27 or 87.037% to 90.741%

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YR(OR)RK

Right Splint will have an R in (OR) gene replaced with a Y gene. Solvents on Right Splint showed:

• RGY
• YORY
• YYOY <--- proves it to be the 2nd last R in genome.

gene targeted is 25/27.

gene range is 24.5/27 to 25.5/27 or 90.741% to 94.444%

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YYOYRK

TEST 2: Vampire/Vampire (Bessodia's Test)

Left: KIYIYIOIYIOIIK 12th gene switched range is 11.5/12 to 12/12 or 95.833% to 100%

R: KIYIYIOIYIOOOK 11th gene is switched range is 10.5/12 to 11.5/12 or 87.500% to95.833%

• I suspect these splints were accidentally switched...

Tay 15

Fuzzy Green Mottled Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 86/Ava 87) Observed:

Left Spoint: no water and no flax!

Right Splint: 1 water, 2 weeds. (one extra weed step)

Left splint had (GYGYGY) gene groups broken in the middle GY Left splint had an(OR) gene group broken.

Solvents on Left Splint showed:

• ORGR
• KORG
• GYRY <-- that pinpoints it as (GYGYGY) --> (GYRYGY)

Left Genome: K(OR)GR(GYRYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 7/27.

gene range is 6.5/27 to 7.5/27 or 24.074% to 27.777%

also reveals that a "G" gene was moved to the right splint

Right Splint:

The phenotype revealed that the (OR) -1 weed gene was mutated.

The right gene results show that an "R" gene was switched w/ a "G" gene. So we are looking for (OR) -->OG

Solvents on Right showed:

• YYRG
• GROY
• RGRO
• GYOR
• OGGR <-- pinpoints as the first (OR) changed.

Right Genome: KOGGR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 2/27

gene range is 1.5/27 to 2.5/27 or 5.556% to 9.259%

TEST 2: Vampire/Vampire (Bessodia's Test)

Left: KIYIIIOIYIOIOK 4th gene switched range is 3.5/12 to 4.5/12 or 29.167% to 37.500% ... a bit out of range w/ Test 1 results

Right: KYYIYIOIYIOIOK 1st gene switched range is 0/12 to 1.5/12 or 0% to 12.5%

Tay 16

Calico, Fuzzy, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 148/Ava 149) Observed:

Left Spoint: no water, 2 weed, no flax

Right Splint: normal

Left splint had the center G or Y in (GYGYGY) broken

Solvents on Left Splint showed:

• RGYR <-- that pinpoints it as the middle G in(GYGYGY) as being replaced with an R

Left Genome: K(OR)GR(GYRYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 7/27.

gene range is 6.5/27 to 7.5/27 or 24.074% to 27.777%

also reveals that a "G" gene was moved to the left splint.

Right Splint had an R in junk genes targeted solvents on Right splint showed:

• GROY
• YYOR
• YYGG <-- pinpoints 19th gene R --> G

Right Genome: K(OR)GR(GYGYGY)GRGY(OR)YYGG(RO)YY(OR)RK

gene targeted was 19/27

gene range is 18.5/27 to 19.5/27 or 68.519% to 72.222%

Tay 17

Calico, Ret, Slimy Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 150/Ava 151) Observed:

Left Spoint: 2 weed, 1 water

Right Splint: normal

Left splint had one of the three (OR) genes broken

Solvents on Left Splint showed:

• YORR
• RYYR
• GYGR
• YYRG
• KORG
• RGYG

the only (OR) gene not coveredis the O in the second genes set

gene targeted was 15/27

gene range is 14.5/27 to 15.5/27 or 53.704% to 57.407%

also reveals that a O was moved to the Right splint.

Right Splint had junk gene targeted solvents on Right splint showed:

• YGYG
• OYYO
• ROYY
• RYYR
• GROY
• GOOR <-- pinpoints 14th gene Y --> O

Right Genome: K(OR)GR(GYGYGY)GRGO(OR)YYRG(RO)YY(OR)RK

gene targeted was 14/27

gene range is 13.5/27 to 14.5/27 or 50.000% to 53.704%

Left genome: K(OR)GR(GYGYGY)GRGY(YR)YYRG(RO)YY(OR)RK

Tay 18

Fuzzy, Mottled Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 152/Ava 153) Observed:

Left Spoint: 0 seeds

Right Splint: normal

Left splint had (RO) broken, Right splint had junk swapped.

Solvents on Right Splint showed:

• ORYY
• RRRO pinpoints it as the G right before the (RO) gene, being swapped to an R.

Right genome: K(OR)GR(GYGYGY)GRGY(OR)YYRR(RO)YY(OR)RK

gene targeted was 20/27

gene range is 19.5/27 to 20.5/27 or 72.222% to 75.926%

Left Splint had R in (RO) gene swapped to a G.

Left genome: K(OR)GR(GYGYGY)GRGY(OR)YYRGROYY(OR)RK

gene targeted was 21/27

gene range is 20.5/27 to 21.5/27 or 75.926% to 79.630%

Sterope Recipes

Sterope 03

Dry, Fuzzy, Simey Aryballa opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 67/Ava 68) Observed:

Left Spoint: 0 weed!! ... got a new (OR) gene

Right Splint: 2 weed !! ... broke a (OR) gene

Solvents on Left Splint (Ava 67) showed:

• ORR
• YGYG
• GYGY
• GYGR
• ORGR
• GRGY
• YORR
• ORYO <-- that pinpoints it as the 6th Y being replaced with an O.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YORG(RO)YY(OR)RK

gene targeted was 18/27

gene range is 17.5/27 to 18.5/27 or 64.815% to 68.519%

Solvents on Right Splint (Ava 68) showed:

• ORR
• KYR <--- proves it. First O gene is replaced with a Y.

Right Genome: KYRGR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted is 1/27

gene range is 0.5/27 to 1.5/27 or 1.852% to 5.556%

TEST 1: Dusk/Ava 26 should swap the 1st R from Ava 26. (if not, next Gene is an R too)

for Left splint, 4 possibilities:

47th gene: GROR --> GRRR north magenta broken, South Cyan gained 48th gene: GROR --> GROR no changes, same gene swapped (R) 49th gene: IOOO --> ROOO broke magenta on outer, inner and middle stamen 50th gene: IOOO --> IROO broken magenta on outer, inner and middle stament, gained cyan on outer stamen

Observed: Left splint (Ava 99) looks exactly like Dusk Right splint (Ava 100) is all white.

Therefore, the 48th gene was swapped from Left splint. 48/73 gene range is now 47.5/73 to 48.5/73 or 65.068% to 66.438%

Sterope 04

Crackly, Dry Reticulated Sabahl opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 88/Ava 89)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• ORYY
• GRGY
• YORY
• ORRK
• RGYO
• OYYO
• RGRO
• RYYR
• RGYG
• GROY

no anomolies found yet. Standard Junk genes 4/27 and 11/27 not covered conclusively.

Solvents on Right Splint showed:

• RGYG
• ORRK
• RGYO
• YRGR

no anomolies found yet. Starting to think this mutagen swapped same genes.

this test is inconclusive (could go back to solvents more)

Sterope 05

Phosphorescent, Prickly, Slimy Sabahl opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 118/Ava 120)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• YYOR
• YGRG
• RGYG
• GYOR
• YORR
• YORY
• RGYO
• KORG
• RGRG
• RGRO
• ORGR
• GRGY
• YGYG
• YYOR
• OYYO

no anomolies found yet. Standard Junk genes 18/27 not covered conclusively. Seems to be that the same genes were switched.

Sterope 06

Calico, Crackly, Spongy Avanya opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 117, Ava 118) Observed:

Left Spoint: 1 more weeding

Right Splint: 2 seeds

Left splint had lost an (OR) gene Right splint had gained (RO) gene

Solvents on Left Splint showed:

• GYGY
• RGYY <-- that pinpoints it as the second (OR) --> YR

Left Genome: K(OR)GR(GYGYGY)GRGYYRYYRG(RO)YY(OR)RK

gene targeted was 15/27.

gene range is 14.5/27 to 14.5/27 or 53.704% to 57.407%

also reveals that a "O" gene was moved to the left splint

Solvents on Right Splint:

• ORGR
• YORR
• RGYO
• GRGY
• ROYR --> points to the 17th gene Y --> O.

Right sGenome: K(OR)GR(GYGYGY)GRGY(OR)OYRG(RO)YY(OR)RK

gene targeted was 17/27

gene range is 16.5/27 to 17.5/27 or 61.111% to 64.815%

TEST 2: Ava 29/Ava 29 (Clarity, Clarity, really)

Left: will target gene 19, 20, or 21. (GROR)(GRRO) (thats ORG in middle) Right: will target gene 22 or 23, both R's in (GRRO)

Observed: Left Splint (Ava 144) gained Cyan South (GRRR) and lost magenta North.

that means gene 19 was targeted.

range is 18.5/36 to 19.5/36 or 51.389% to 54.167%

combining with test 1 results, the new range is 53.704% to 54.167%

Right Splint (Ava 145) gained an O gene. Dont know where w/o using a solvent.

Celano Recipes

Celano 01

Dry, Spongy, Spotted Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 90/Ava 91)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Left Splint showed:

• RGYO
• GYGR
• YYRG
• GYOR
• KORG
• YORY
• YGYG
• GYGY
• ORGR
• GROY
• RYYR
• RORG
• YGRG

covered all of the genome except the last 4 genes. Not showing anything yet, so stopping to save solvents... no conclusions drawn yet

Celano 02

Green, Mottled, Prickly Renard opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 92/Ava 93) Observed:

Left Spoint: normal

Right Splint: 2 weed, 1 flax 1 water.

Left splint had no gene groups broken Right splint had (GYGYGY) broken (but not middle GY)

Solvents on Right Splint showed:

• RYYR
• YYYG <-- that pinpoints it as the third G directly in(GYGYGY) as being replaced with an Y

Right Genome: K(OR)GR(GYGYYY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 9/27.

gene range is 9.5/27 to 10.5/27 or 31.481% to 35.185%

also reveals that a "G" gene was moved to the left splint

Solvents on Left Splint:

• GYGY
• GYGR
• RGYR --> points to the 17th gene Y-->G.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)GYRG(RO)YY(OR)RK

gene targeted was 17/27

gene range is 16.5/27 to 17.5/27 or 61.111% to 64.815%

TEST 2: Ava 24/ Dusk (actually Ava99, which is exactly Dusk genome)

gives Ava 135 / Ava 136

expect: Left splint will donate gene 11 or 12. both are G's. Ava 24 genome: RRROOOYYGGGGUUUIII

Right Splint: should be gene 23 to gene 26: (gene set IYYY) if 23, IYYY --> GYYY lose yellow on entire stamen, gain S yellow if 24, IYYY --> IGYY lose yellow on entire stamen if 25, IYYY --> IYGY lose yellow on inner and middle stamen if 36, IYYY --> IYYG lose yellow on inner stamen.

Observed: Ava 136 looks like Dusk but with yellow on South petals, and one less yellow shade on all stamen parts. therefore, gene 23 of 73 was targeted! genome: GRORGORRUYYYGYORIYGRROGYYYGRROGORRGRORROYGIOGRORIOOOIYYIYURRRGRROGORRUOOO range is 22.5/73 to 23.5/73 or 30.822% to 32.192%. combining with Test 1, new range is 31.481% to 32.192%

Left splint needs solvent to determine which gene was switched.

Alcyone Recipes

Alcyone 01

Calico, Crackly, Pricky

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. (Ava 105/Ava 106) Observed:

Left Spoint: had an extra weed step (OR) was broken

Right Splint: no change, must have mutated junk

Solvents on Left Splint showed:

• GYGY
• GYGR
• GRGY
• YRGR
• OGYY <-- that pinpoints it as the 4th R being replaced with a G.

Left Genome: K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

gene targeted was 16/27.

gene range is 15.5/27 to 16.5/27 or 57.407% to 61.111%

also reveals that a "G" gene was moved to the left splint

Righgt Splint:

The right gene results show that an "G" gene was switched w/ a "R" gene, in the junk sections

Solvents showed:

• KORG
• YORY
• RGRG
• YGYG
• YYRG
• ORYY
• YORR
• GRGY
• RYYR
• YYOR
• ORRK
• YGRR <--- pinpoints as 6th G being replaced w/ an R.

Right Genome: K(OR)GR(GYGYGY)GRRY(OR)YYRG(RO)YY(OR)RK

gene targeted was 13/27

gene range is 12.5/27 to 13.5/27 or 46.296% to 50.000%

AC1 02

Green, Phosphorescent, Smelly Avanya opened

TEST 1: Nile Green / Nile Green Unmutated Genome:

K(OR)GR(GYGYGY)GRGY(OR)YYRG(RO)YY(OR)RK

1 weed/1 water/1 seed/2 flax/0 rotten

applied mutagen. Observed:

both new splints had same phenotypes. (Ava 131/Ava 132)

Both splints had junk genes swapped, or same type of gene swapped.

Solvents on Right Splint showed:

• YYOR
• RYYR
• GYGY
• YGRG
• GRGY
• YORR

no anomolies found yet. Standard Junk genes mostly covered

this test is inconclusive. Could do further solvent testing, but at this point, no results.

TEST 2: Ava 29/Ava 29. (Ava 29 has 4 junk genes, otherwise, its just a clarity bulb) Ava 26 genome: IRIRIOIOIYIYGORUGRORGRROGORRGRORGRRO

mutagen applied: gave Ava 142 / Ava 143

Right Splint: observed: north petal lost a cyan (now is 2 red shades), and outer stament gained 1 cyan (+IR)

The GORR gene set was targeted (to break North cyan) and the first R was switched to an I, to give GOIR.

Right Genome: IRIRIOIOIYIYGORU GROR GRRO GOIR GROR GRRO

27th gene of 36 was targeted

range is 26.5/36 to 27.5/36 or 73.611% to 76.389%

Left Splint: observed outer stamen lost 1 yellow an IY gene was targeted, and it lost the I.

so IYIY became either RYIY or IYRY

either 9th or 11th gene targetd

range is 8.5/36 to 10.5/36 or 23.611% to 31.944%

could narrow this down by testing w/ solvents

Crosses for Lily Requirements

Aiming for 75% Green petals

Stragety: Add cyan to one set of inner petals on Morning, then break one of the two yellow genes for that same set. This will turn that inner set of petals green. The outer two sets of petals are already green, so that will be 3/4 green.

CROSS 1: Morning / Crown with calico,dry, spotted (Sterope 01) Aiming to swap first genes of both splints. Unmutated Morning:

K(IRRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Cyan1OSMSIS(IRRR)+Yel2OSMSIS(IYYY)+Yel2IEP(UYYY)+Yel2IWP(UROR)+Cyan1OSP(GRRR)+Yel1OSP(GYYY)+Cyan1ONP(GORR)+Yel1ONP(GRRO)

Unmutated Crown:

K(UROO)(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Red3=[Mag3IWP(UROO)+Ye3IWP(UROR)] + 5V

This mutagen will Turn (IRRR) (cyan on stamen) into (URRR), cyan on Inner East Petal.

Applied mutagen. (Ava 45/Ava46) Observed:

Left Spoint(Ava 45): Inner east petals now greenish yellow. the cyan add was a success!!

Genome of Ava 45: K(URRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Right Splint(Ava 46): Inner petals look orange, not red

Genome of Ava 46: KIROO(UROO)(UROO)VURVOOVURVORV(UROR)(UROR)(UROR)K

Right splint had first gene broken, UROO (magenta inner petals) --> IROO upper stamen cyan 1. inner petal set now 3 yellow shades + 2 magenta shades = red + red + yellow, or a dark orange

CROSS 2: Ava45 / Blush with Fuzzy, Hairy, Spongy (Tay 06)

aiming to break up a (UYYY) inner yellow gene on Ava 45

Mutation should swap gene 18/44 (Y) on Ava 45 with gene 10/26 (V) blush. Unmutated Ava 45: K(URRR)(IYYY)(IYYY)(UYYY)(UYYY)(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K Unmutated Blush: K(GROR)V(GOOO)V(UROO)V(IO)V(IOO)V(IOOO)K

Ava 47 / 48 resulted. Observed: left splint (Ava47) does have predicted colors. Green on outside petal sets. one green and one yellow inner petal sets. right splint (Ava48): colors appear to be blush. No observed change.

Hypothesized Ava 47 genome:

K(URRR)(IYYY)(IYYY)(UYYY)UYVY(UROR)(UROR)(GRRR)(GYYY)(GORR)(GRRO)K

Hypothesized Ava 48 Genome:

K(GROR)V(GOOO)Y(UROO)V(IO)V(IOO)V(IOOO)K

both are consistent with expected and observed results

Aiming for Cyan on Inner Stamen

Maia needs this flower for Xenobotany

Test 1: morning/vampire (Ava 127, Ava 128) Mutagen Sterope 03

Expected: should convert a "GRRR gene to IRRR", which would be the second IRRR gene in morning. Vampire should lose one IY, since it is donating an I, turning it into GY.

Observed: results are as expected. Vampire has 3 magenta 2 yellow on outer stamen, instead of 3 mag, 3 yellow. Right genome: GYIYIOIYIOIO

Morning lost some cyan on south petal, but inner stamen is now two shades of green. Left genome: IRRR IYYY IYYY UYYY UYYY UROR UROR IRRR GYYY GORR GRRO

tried to donate to Univ., but they said its not acceptable. That means probably can't have any OTHER inner stamen colors. Next step is to remove the two yellow shades then

Test 2: vampire/Ava 127 Tay09 (Calico, Green Smelly)

Vampire should donate 6th gene, O. Ava 127 will hit either gene 9, 10, 11 in IYYY

• if 9, I in IYYY will be targeted --> OYYY removing 1 yellow from entire stamen
• if 10, first Y in IYYY will be targeted --> IOYY removing 1 yellow from entire stamen and gaining magenta on outer stamen
• if 11, second Y in IYYY will be targeted --> IYOY removing 1 yellow from middle and inner stamen

Mutagen applied, giving Ava 154 / Ava 155 Observed: Left Splint, Ava 154 has gained yellow on outer stamen. It was a Y it gained. genome is: IYIOIYIYIOIO

Right Splint, Ava 155 lost yellow on entire stamen and gained magenta on outer stamen. Therefore, 10th gene was targeted. Genome is IRRR IYYY IOYY UYYY UYYY UROR UROR IRRR GYYY GORR GRRO range is new for RIght splint range 9.5/44 to 10.5/44 or 21.591% to 23.864% first yellow shade was removed successfully